EMAGE mouse gene expression spatial database

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EMAGE:14279

BC004004 cDNA sequence BC004004 ( MGI:2136782)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004080. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004080_01	euxassay_004080_02	euxassay_004080_03	euxassay_004080_04

euxassay_004080_05	euxassay_004080_06	euxassay_004080_07	euxassay_004080_08	euxassay_004080_09

euxassay_004080_10	euxassay_004080_11	euxassay_004080_12	euxassay_004080_13	euxassay_004080_14

euxassay_004080_15	euxassay_004080_16	euxassay_004080_17	euxassay_004080_18	euxassay_004080_19

euxassay_004080_20	euxassay_004080_21	euxassay_004080_22	euxassay_004080_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14279_wholemount_strong.wlz

14279_wholemount_moderate.wlz

14279_wholemount_weak.wlz

14279_wholemount_possible.wlz

14279_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14279_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5595
Entity Detected:	BC004004, cDNA sequence BC004004 ( MGI:2136782)
Sequence:	sense strand is shown >T5595 GCCCGAGAGGGCGCGGGCCCGCATGGTTAAACCACTGTTAAGGGATCAAGCTCAGCCTTCTGAACTTCCC TCCGAGGCAAGCTCCCTTCTGCCAGGCTGGACCCTCACTCTGGAGGGACTTCATTTTGGAAGGCATGGAT CTTGCTGCTAATGAGATCAGCATCTATGACAAACTTTCAGAGACTGTTGATTTGGTGAGACAGACAGGCC ATCAGTGTGGAATGTCCGAGAAGGCAATCGAAAAATTTATCAGACAGCTACTGGAAAAGAATGAACCTCA AAGGGGACCACCCCAGTACCCTCTCCTTATAGCCGTGTATAAGGTCCTCCTAACTCTGGGATTAATCTTG TTCACTGCCTACTTTGTAATTCAGCCTTTTAGCTCATTAGCACCCGAGCCAGTGCTTTCTGGAGCGAACA GCTGGCGCTCACTCGTTCATCACATTCGGCTGGTGTCCTTGCCCATTACCAAGAAGTATATGCCAGAGAA TAAGGGCGTTCCTCTGCAAGGGAGCCAGGAGGACAAACCCTTCCCAGACTTTGACCCCTGGTCATCATAT AATTGTGAGCAGAACGAGTCAGAGCCCATCCCTGCCAACTGCACTGGCTGTGCCCAGATATTACCCCTCA AGGTAACGCTGCCAGAAGACACTCCAAAGAATTTTGAGAGACTCCGGCCTCTGGTGATCAAGACAGGACA GTCTCTGTCGTCTGCAGAGATTCAGAGCTTCTCATGCCAGTACCCTGAAGTCACAGAGGGCTTCACTGAG GGCTTTTTCACCAAGTGGTGGCGCTGCTTTCCTGAGAGGTGGTTCCCATTTCCTTACCCATGGAGAAGAC CTCTAAACAGATCACAGATTTTACGGGAACTTTTCCCTGTTTTTACCCAACTTCCATTTCCAAAAGACTC CTCCTTAAACAAATGTTTCCTGATCCAGCCGGAACCTGTGGTGGGTAGCAAGATGCATAAGGTGCATGAC CTGTTCACCCTCGGCAGCGGGGAAGCCATGTTGCAGCTCATCCCTCCCTTCCAGTGCCGAACACACTGTC AGTCCGTGGCCATGCCGATAGAGTCTGGAGATATTGGCTATGCCGATGCTGCCCACTGGAAGGTCTACAT TGTAGCCAGAGGGGTCCAGCCTTTGGTCATCTGTGATGGAACCACTCTCTCGGATCTGTAGGAAACAACT GCTTGGAGACCTGAAACTCAGGCTGAAAAAGAGTGCGGAGCGGGGAATAAAAACACTGAAACACTTTGCA TGGGTTGGTTGCTTAGTTACCTGCCCTGGATGTTTTGGCTGTCAGCTGTATGCTGGAGACAGTGGCTTGG GCCTAGCCATCTTGTCTGTCTGTAGCTTCCTCAGGAAAGATGGAAGCCTGGCTGCTGGCCAAGACTTCTC TCATTTCTCCTCCCCCAGGCTTTGGACTCCCAGGTATTGGAAATAGGCTGTGGCTCTTTGACGTGTGTGG ACATGGCTCCTCTACTGTCTTCTTGGCCGTGGAGGGCAGCAGTGGGTCCTGATTCTGTTTGTCCACTCTT CCTTGCCGCATCACGCTGTACTTGGTGCGAAAGCCATAGCTGACTTTGATCACTACAGTTTGACAAGGAA AGTGAATTTGGAACCTGGGGGGCTTGGGGGGTGGGGAGGGACAGATTGGATAGCTGGAATTGCATGTTTA AAGGTGCCTGCACCTGTGTCGTGCTGCACACCTTTAGTCCCAGCACTTGGGAGGCAGGTGCATATCTTTT GAGTTCTAGGCTAAGCTGGTCTCCATAGTGAGTTCCTGGTCTCCAAGTGTTACATAATGAGACCCTGTCT CAACACTGTTCCACCACTCCCCCTGCGCCCCCCAGATCTAGGGATGTAGCTCAGTGGTAGAGTGCTTCCC AGACACGTTTGAGCCCAGAGTGCAGCCCTCAGCATCATGAAGAGGAGGACAGTGTACATCTTACATAATG TCCATGGCGCCTGGAGTTTCTGTAAGGGGTGCTGGAGACTGGGTTACAATTCAAGTCTTACTAAATCCTG GTTTCCTCCCACACTGCCATCAGATTGTTTTCTCAGAAGTTCGGCCTCCCCCAGCTGGCCAGCACCCTCT CTGCTAAGGCAGCAGAGCCTTTGTCAACTGCTGCTGTACCCCACCCCCTTCAGAAGGAATGAGTTAGAAT AGCGGGCCTGAGGGAGACAGGGCACCTCCCTGAGTTTGATCTTTAGGCCTGACTTTCTACAGAGATGTCA GCAGCCTCAGAGCTGATGTAATGTGGTCGAGGGAGGAGCTGCAGAGCCAGAGAGCCGGAGCGTTTGCTGC AGGGTCTGCGGCTCACTTCAGGGCCCTCTGCCCTACCTTTTCTGTACCAGGGCAAGGTGAGATATTGGTT TAGTTGAGATGCAGGAGTATCCTAAATAATATTCTGTGCTTACTGCACCCCCAAGTGCTTTGGGGGTGTT CTTATCAAACAGTGCAAAAAAGGATGGTCTGCCCTCTTGTTCTTTTTTGGGGTCTTGCTGTGTAGCTCTG ACTAGCCTCGAACTCATGTAGACCAGACTGGCCTCAAACTCACAGAGATCTGCCTGTGCCCCAAGTGCTG AAAATTAAAGGAGTATACCATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6488611 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6488611 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14282 same embryo
	EMAGE:14281 same embryo
	EMAGE:14280 same embryo
	EMAGE:14283 same embryo
	EMAGE:14278 same embryo
	EurExpress:euxassay_004080 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source