EMAGE mouse gene expression spatial database

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EMAGE:14283

Zfp612 zinc finger protein 612 ( MGI:2443465)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004094. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004094_01	euxassay_004094_02	euxassay_004094_03	euxassay_004094_04

euxassay_004094_05	euxassay_004094_06	euxassay_004094_07	euxassay_004094_08	euxassay_004094_09

euxassay_004094_10	euxassay_004094_11	euxassay_004094_12	euxassay_004094_13	euxassay_004094_14

euxassay_004094_15	euxassay_004094_16	euxassay_004094_17	euxassay_004094_18	euxassay_004094_19

euxassay_004094_20	euxassay_004094_21	euxassay_004094_22	euxassay_004094_23	euxassay_004094_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14283_wholemount_strong.wlz

14283_wholemount_moderate.wlz

14283_wholemount_weak.wlz

14283_wholemount_possible.wlz

14283_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14283_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5596
Entity Detected:	Zfp612, zinc finger protein 612 ( MGI:2443465)
Sequence:	sense strand is shown >T5596 CCATTGCGGTTCGCGGTGTGTCTTGGCGGACAGCACGCAGGGGCTCCCGCAGCCAGCCTCGCGGGCCACC AAGTCTCCGTGTCCACAGCGGTGACGCTTGAGTGGCGACCATCGTCAGACCCAGAAAACTCAGCAGGTGG CGGATCGGGGAAGCTGTGTCACTTCACAGAGGGGTGGCTGCGTGCGGGGGCGAAGCGGCTCGAGGTGACA TCGCAGGTCTCTTGACCCGCCGCGGCCCTGCAGCTCCAGTCCTTCCTTGAGAGGGAGACCAGGTGACAGC AGCCATGATCCCATCGGTTTGGCCCCAGAAGTCGGTGACCTTTGAGGACGTGGCTGTGTACTTCACCCAG ACGGAATGGGATGGTCTGTCTCCTGGGCAGAGAGCGCTGTACAGGGATGTCATGCTGGAGAACTATAGGA ACCTGGCCTCTGTAGGATTTCCACTTCTCAAACCTGCTGTGATCTCACAGCTAGAGGAACGAAAGGATCT GGAAAACCTGTTTCAGCTGGCCACTGGAACGGACTCCCAGGGCCTCTGGACTGAATGTACTGCTGTCCAG TCCGGCAATAATCTGACAAAAGAAGTGCATGAAGAAGAAAAGAGTACATTATTTGAACTTCAGAAGGACT TTTCTCAGGAAACAAACTTTTCAAAAACGTATATTTTGGGCCAACAACAGGAAATCCGGTCAGCAGGAAG TGAGAGGGGGAGTGCCAGTGCCACCGATGGAAGAGCTAAGGCGAGCTCTGTGGAAGGGTGTTTGTTTAGT CAGACTCCACAATTGTCCCAGGATCATACCAGCTCCACTGGAGAGCTATATTCTGATACCAAACTTAGTA AGAGTGAAAGAATCACTACAGAGGAAAGACCTCTGAAACCCAGAGAATTAGCAGAGGCTTCTCAAGGCAG CCCTCAAGTTAATCAGCTTCCAGAGATCTGTGATGTGGAGAAGCCCTACCAGTGTGCAGAATGTGGCAAA GCCTTCAGTGTTAAAGCCAAGTTCGTCTGGCATCAGAGACTTCATAATGGAGAGAAACCTTTCAAATGTG TGGAATGTGGGAAGTGCTTCAGCTACAGTTCCCACTACATCACACATCAGACAATCCACAGTGGAGAGAA GCCTTATCAGTGTAAGGTATGCGGCAAGGCCTTCAGTGTTAATGGAAGTCTCGTTAGACACCAGAGGATC CACACAGGAGAGAAGCCGTATCAGTGCAAGGACTGCGGGGCTGGCTTCGGCTGCAGTTCTGCGTACATTA CTCATCAGAGAACCCACACTGGAGAGAAGCCTTACGAGTGTAGTGACTGTGGGAAAGCCTTCAATGTTAA CGCAAAACTGATTCAGCATCAAAGAATTCACACTGGAGAGAAGCCTTATGAGTGCGATGTGTGCGGGAAG GGCTTCCGGTGCAGCACCCAGCTTAGGCAGCATCAGAGTATACACACTGGGGAAAAGCCGCATCGCTGCA GCGAGTGTGGGAAAGGCTTCACTAAAAATGCAAAGCTCATTCAACATCAGCGAGTGCACACGGGGGAGAA GCCCTACGCCTGCAGTGACTGCGGAAAGACTTTCAGTGCCAAAGGGAAGCTGATCCAGCACCAGAGGATC CACACGGGCGAGCGGCCGTATGAATGCAGTGAGTGTGGGAAGTCTTTCAGGTGTAACTCCCAGCTTCGGC AGCATCTGAGAATCCATACTGGGGAGAAGCCATATAAGTGCAGTGAGTGTGGCAAGGCCTTCAACGTCAA TGCGAAGCTGATGCAGCACCGGAGAACCCACACCGGGGAGAAGCCCTTCGAATGCAAGGAGTGCGGGAAA TGCTTTACTTCTAAAAGAAACCTGCTGGATCACCAGCGAGCCCACACCGGAGAAAAGCCGTATCAATGTA GCGAGTGTGGGAAAGCTTTCAGTATCAATGCCAAGTTAACCAGACATCAGCAAATACATACCAGGGAGAA GCCTTTCAAGTGTATGGAATGCGAGAAAGCGTTCAGCTGTAGCTCTGACTATATTGTGCACCAGAGGATC CACACTGGAGAGAAACCCTTTCAGTGTGGCGAGTGTGGCAAAGCCTTCCACGTCAATGCTCACTTAATTA GACACCAGAGAAGCCATACTGGGGAGAAACCTTTCCGGTGCACAGAATGTGGCAAGGGCTTCAGCCTTAG TTTTGACTGTATTATACATCAGACTGTCCATACTTGGAAGAAACCCTATGTATGTAACGTATGTGGGAAA ACTTTCAGGTTTAGCTTCCAACTAAGCCAGCACCAAGATGTTCACAATGAAGACAAATCCTAATGAAGAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6488946 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6488946 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14279 same embryo
	EMAGE:14282 same embryo
	EMAGE:14281 same embryo
	EMAGE:14280 same embryo
	EMAGE:14278 same embryo
	EurExpress:euxassay_004094 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source