EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14308

Pip4k2b phosphatidylinositol-5-phosphate 4-kinase, type II, beta ( MGI:1934234)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004158. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004158_01	euxassay_004158_02	euxassay_004158_03	euxassay_004158_04

euxassay_004158_05	euxassay_004158_06	euxassay_004158_07	euxassay_004158_08	euxassay_004158_09

euxassay_004158_10	euxassay_004158_11	euxassay_004158_12	euxassay_004158_13	euxassay_004158_14

euxassay_004158_15	euxassay_004158_16	euxassay_004158_17	euxassay_004158_18	euxassay_004158_19

euxassay_004158_20	euxassay_004158_21	euxassay_004158_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14308_wholemount_strong.wlz

14308_wholemount_moderate.wlz

14308_wholemount_weak.wlz

14308_wholemount_possible.wlz

14308_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14308_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

moderate

regional

moderate expression: see section 11 12 13

telencephalon

moderate

regional

moderate expression: see section 05 06 07 08 10 14 15 16 17 18 19 20 not examined expression: see section 11 12 13

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 weak expression: see section 07

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

mandible

moderate

regional

moderate expression: see section 05 weak expression: see section 06 07 17 18 19 20

temporal bone petrous part

strong

regional

strong expression: see section 04 05 06 07 15 16 17 18 moderate expression: see section 08 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5612
Entity Detected:	Pip4k2b, phosphatidylinositol-5-phosphate 4-kinase, type II, beta ( MGI:1934234)
Sequence:	sense strand is shown >T5612 GAGGCAGCGGGGGTGGGGATCGGTTCGGGGGAGGCCTGGGGCCGCTCGCTGGTGCGCTGTCTCCGCCGCC CCCCCTTTCGCCGCCGCCGCCGCCCCGGGCATGTCGTCCAACTGCACCAGCACCACGGCGGTGGCGGTGG CGCCGCTCAGCGCCAGCAAAACCAAGACCAAGAAGAAGCATTTCGTGTGCCAGAAAGTGAAGCTATTCCG GGCCAGCGAGCCGATTCTCAGCGTCCTGATGTGGGGGGTGAACCACACGATCAATGAGCTGAGCAATGTC CCTGTTCCTGTCATGCTAATGCCGGATGACTTCAAAGCTTACAGCAAGATCAAGGTGGACAATCATCTCT TCAATAAGGAAAACCTCCCCAGCCGCTTCAAGTTCAAGGAGTACTGCCCCATGGTGTTCCGAAACCTCAG GGAGAGGTTCGGCATCGATGACCAGGACTACCAGAATTCAGTGACACGCAGTGCACCTATCAACAGTGAC AGCCAGGGCAGGTGTGGCACTCGCTTCCTCACCACCTATGACCGGCGTTTCGTCATCAAGACTGTGTCAA GTGAGGATGTGGCCGAGATGCACAACATCCTAAAGAAATACCACCAGTTCATAGTGGAATGTCACGGCAA TACCCTCTTGCCACAGTTCCTGGGCATGTACCGCCTGACCGTGGATGGGGTGGAAACCTACATGGTGGTC ACCAGGAATGTATTCAGTCATCGGCTTACTGTGCATCGCAAATACGACCTGAAGGGTTCCACTGTTGCCA GAGAAGCCAGTGACAAGGAAAAGGCTAAGGACTTGCCAACTTTCAAAGATAATGACTTCCTCAACGAAGG GCAGAAGCTGCACGTGGGAGAAGAGAGCAAAAAGAACTTCCTGGAAAAACTGAAGCGGGACGTTGAGTTC CTGGCCCAGCTGAAGATCATGGATTACAGTCTCCTGGTGGGCATCCACGACGTGGACCGGGCAGAGCAGG AGGAGATGGAGGTGGAAGAGCGAGCGGAGGAGGAGGAATGCGAGAATGATGGGGTGGGTGGCAGCCTGCT CTGCTCCTATGGCACGCCTCCAGACAGCCCTGGCAACCTCCTGAGCTTCCCCCGGTTCTTCGGCCCAGGA GAGTTCGACCCCTCTGTGGACGTCTATGCCATGAAAAGCCATGAAAGTGCCCCTAAGAAGGAGGTCTATT TCATGGCCATCATTGACATCCTCACACCCTATGACGCTAAGAAGAAAGCTGCACACGCCGCCAAAACGGT GAAACATGGGGCAGGGGCTGAGATCTCCACTGTGAACCCGGAGCAGTACTCAAAACGCTTCAACGAGTTC ATGTCCAACATCCTGACGTAATCATCTCTGCCTTCTGCCAGAGCCAGACTGCTGGACCAGGGGTCGGGGA TCGGGAGAGGGAGAGGGTATATCTGGGCCGGGAGGGTGGGGAGCAGAGTGGGGGCTTGCGAGGGCTTTAG TTAAGGGACTGCAGCCTGTGACCTGAAGGAAGAAGGGGACGGGCTGTTTGCACGTGCTCACAGGATGCAG CCTTACTTCCCGAGCACCCTCGATCCGCCTCTTTTGCCCCTGGTTAAAACAGGGTTTCCTCTTCGGTATC TCCGTAACCTATGATACCCGGGAGCTGGAGTTGACTTTGTGGGTTTCTTTGGGTTTGTTTCTTCAATTCA CTGCTCCAGGATTGCTCTTTCTAATGCTGTATGTCATCACCCTCGGGGCCCTCCCTCTTCCTGTCAGGCT CTCAGTTCCCTTCAGTTCAGGCCAGCACAAGGGACGAGAAGAGCAGTTGCTCAGACACCTCAGTCAGCCA CGGTTTGTTCTCACCTAGATGGACTCTTTTATTTCCTGGAGTATTGTGCATCACCCCTCCCCCCCTCATG CTTCCACCTTGGAGCCAAGTGAAACCTTGGCTCCCTCACCCCATCCTCACAGAAGAACATGGCAGGTTGA CCCTGACGCCTTGGACCGGAGTCAGAGGCCTCAGAGGGGAACAGGTTCGTCTTCATGAGACGGTGAATGA CCAGGAAGCAGCCCTCAGACAGCCGGTTCTCAAAGAACCCAGAGATCACCACCCCGGCCACCTGCGCCTC TGAGTGGAATTCTGATGTCTGTCAAGGCCGCCACCCACCGGCTCCAGAGGAACTATCAAGCATCGTGGCA TTTATTGGGGCCCCTGTGTTTCGATGACTGTTTCCCTTTGGGAAAATCCCAGAAACTAAGATGTGTAAAC CGGGCCCGTATGCACCCTCCATATCCTAACTACTGACCTCTGGGTGCAGTCGCCTGTAACTGGCAAATGG TCAATGCAGAGCTCATCGCAGAGCTGCCACTCCAAGCCTGGCCCGCCGTGCAGGTGTGTCTGGCTCCTGT GCCTGTGACTTCTCACCTGGACAGCTTTAGCCAGCAAGGTGCTCCACCTGAGGTTGCCTGAGTCACCTGA GGAGGTGACCCCAGGAAAGCCTGTTTGTTTGGAGGCCTGACACGGGGCTTTGCCCATCCCCCAGCCCCAG GGCTGACGGATTTTCCTTGTCACCCTCACTCTTTCTGGACTTGGGATCTGCCCCCGGTCCTCCTTGGAAA GAGTGACAGCCCGCAGCCTCCTTCAGGATTCTGGCCCAGGGCCTCAGAAGGGAGCTACACAAGTCATTCT GATGCCACCAGAGCAAGTGGTTGCCAGGAAGAAAAACAAAACGGTCTCGCCGGCCTGCCCTTGGAGGAGG TAAACTCCCTACCTCTTCCCAGCTCCCTCAGGAGGAATCCGGAATTTAGGATGAGATGGATTTTTGTAGA TAACCCCAAAGATGTGAAGCTGGTTTGTGAAGCTGTCGTGGGTGAATAGATGATTCCCAACGCCTGCCCC CCAAACTGGCCCAGCTCAGCTTGGGGAGCTGGGGAGTGGCCACTGTGCCGTGTCCTCCCTATCCCTGCAC TGAACTAAGACAGGAAAAGCAAGATGGCAGTCTTGGGCTTCTCTGGGGCTGCCAATTGGGTGAGGCAGGT GGGGGCCCACTCCTTCCCAGGAAGAGAACCAGGTAAGGTTCTGGTGGGCAGAACCACGGGGGACTTGGGG AAGGACTCAGAAACCATGTGATTCGAGGAGAAAGGATTTTAATGTAGGAGAGGGGTTACGTAGGAATAAT GTCTGGACTTGATTTCTCACCTCACAATCAAGAGTAAGGACTCTGGATCTGCCCCAGGCTTGGAAGTCGA AGCTCCTGGCTGATCCTGTTGTGTGTTTGCAGCCGGCTAACTGGGTTAACGGTGGGATTTGGGGGGGGGG TTATTTCGTTTCGTTGTATATTGTTTACAATTTTGAGAGGTAGCTTTCTGTTTCAAGCTCTCTGTTTTCC TGACAGTATTGTTGAGTGGGTCAAAATATTCAAGCTGTGGTTTGGTGGATTTTAGGTTTGTTGTTTTTTT GTTTTGTTTTGTTTTGTTTTTTAGGTCATTCTCTGCGCCATCTTCAAAACCCTGAGGTTTGACTTTCTGA TTCCTTGGCATTCATTCGTGTGTTTAAAAGCTATTTATCTTCATTTCAGACAAGTTTTCTTTCTCTTTGT GACAAAGATGTGCTATTTGGCTTCAATCTCCACATAACCCCCTCCCCCCATTTATCTGCTCCCCTCTTCC TGAGTCCCACCAGTCACGCTCTGGGCAGCAGGTGCCAAGGGGAAGACAAGACCCTTAGCTTGACTCCTCA GGGCCGTGAAGAACAGCCAGACTGTCTTATGGCATCCAGAATCATGTTGGGTCTTCCAGAAATAGACATT TAAGAATAGAGTCCTGGACAACTGGGTGAGGTCTGGATGCGGGGAAAGGGGGGGATTCCATTCCCAGGCC CTTACCCTCCCGATTACTGTGTGGCAGCCAGTCCCCATTCCTTCTGTGGAAACTGTGGAACTGAGAAAAT ATGTCTCGCATCCAGCTGACACAAGGGATACTATGAACACTCTGACACCAAGCAGTGGCCTCAGGCCGTG TTCCCAGTCTGCAGGGCAGCGCCAGGAGTGCGGTTCAGTTCCGCTGCAGTGCTAAATCCTTATCTAGCTC AGGGGTTACTGGTCTGTGGCTGGCGCCATGAGTGGCCCCCAGGCTGTTCTCAGTGGCACTATAGTCCCCC ACCCCACCCCCAGGCAGCTGTCCCCCTCCTTACCCCCCTCCCCCCAAAATCACTTTTCTCAGTCCTGGGC TTTAGGCCTGAACAGGTCCCCTAGCAGTGCTCAAGGGAGGAGAGACTGGACTCCTGTCGAGACTTCCCCC CTTCACCATGTGCCAGGCCTCCCTGGGGAGGTGGACCAAAGACCCAGGACTTTCTCAGTAGCCAGTCCCC CACCCCACCCCACCCCAACCCCAGTTGTCTTTTTAACAATTCAGGTTGAGAGGGAAAAACCATTAGCTGT TTCTAGTGCTTGACCGACTCAAGTATTTCGGGCAGAAAGGACAGTGCACCGAGACAGCCCTCAGAGCTCT GGCCTTGTGGTTTCCCAAGTTTAGAGACCTCACCAGCAGTAATGGCCGGTGGGGTGGGGGTAGGGGGTCT CAAAGGGAAACACATTTAGACAGCTAGCCAAAATCTGCCAGCCGTGGTCCCAGTCCTCTGAAGTGTGCCA CTTAGGATGAGTGTCACCAAGGGGAAGTGAGACGTAGCTGTCCTTCAGGTTGAGTCACCAGGAAAGAGGC TGAGGACACCCGAAGAGCCTGTCCCTTGCCCACAGCCATTTTTCAAGGTGGGAGCCCATACATTTAGGGG GACCATCTGCTTAGCACATCTCAGCGGCTGGTCAGAACATTGCACACACGAAATGTGAATGAAATCTAGA GTCATCTTTGGGCCCGCAGGATTAAAGTTCTACCCACCACATGTGACTTGTCCATTTCCGGCTGCAAGCT CCATTTTTTTAAAAATAGGAATTTTCACCCCTGTGCTTGTCTAATGTCTGCTCTGAACAGTTCGAGCCTC TGTTACTGTTTTAAAATGCATGCATGTTAAAAAAGAATCTCCAAATGGGAGGACAAAATAAAACTCGAAA AGCTTTGTGTACAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6491155 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6491155 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14307 same embryo
	EMAGE:14304 same embryo
	EMAGE:14309 same embryo
	EMAGE:14306 same embryo
	EMAGE:14305 same embryo
	EurExpress:euxassay_004158 same experiment
	MGI:4827215 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source