EMAGE mouse gene expression spatial database

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EMAGE:14324

2510003E04Rik RIKEN cDNA 2510003E04 gene ( MGI:1919570)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004152. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004152_01	euxassay_004152_02	euxassay_004152_03	euxassay_004152_04

euxassay_004152_05	euxassay_004152_06	euxassay_004152_07	euxassay_004152_08	euxassay_004152_09

euxassay_004152_10	euxassay_004152_11	euxassay_004152_12	euxassay_004152_13	euxassay_004152_14

euxassay_004152_15	euxassay_004152_16	euxassay_004152_17	euxassay_004152_18	euxassay_004152_19

euxassay_004152_20	euxassay_004152_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14324_wholemount_strong.wlz

14324_wholemount_moderate.wlz

14324_wholemount_weak.wlz

14324_wholemount_possible.wlz

14324_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14324_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5634
Entity Detected:	2510003E04Rik, RIKEN cDNA 2510003E04 gene ( MGI:1919570)
Sequence:	sense strand is shown >T5634 ACGGACGGGGAGGCGGCCGTCAGCTCAGGCCGCGATGGCGAACGCTCCGGGGCCAGAGATCCGCGAGAAG TTCCAGGCAGCGCTGGCCCTGTCGCGGGTGGAGCTGCACAAGAACCCGGAGAAGGAGCCGTACAAGTCCA AGTACGGCGCGCGCGCGCTGTTGGAGGAGGTGCGCGCGCTGCTGGGCCCGGCGCCCGAGGACGAGGACGA GCCGGCTGCTGACGATGGGCCGGGCGACCAGGCCTTGGGCGCGGGTGAGCCCCGGGAGGCTGAGGGCCCC GGAGCGCAGCGGGCGCTGCGCCTCGCGGTGGTCGAGTTCCACCTTGGTGTGAACCACATCGACACGGAGG AACTGTCTGCCGGCGAGGAGCATCTGGTCAGGTGCCTGTCGTTGCTGCGCCCCTACCGCCTGTCGCTCGG CTGTGTGTCTCTCTACATCCAGGCGCAGAATAACCTGGGCATCCTGTGGTCTGAAAGGGAAGAGATCGAA ACCGCCCGGACCTACCTGGAGTCATCGGAAGCCCTGTACAGTCAGTACATGAAAGAGATTGGGAGCCCGC CTCTTGACCCGACTGAGCATTTTCTTCCTGAAGAAGAGAAACTTACTGAACAAGAGAGATCAAAAAGATT TGAGAAGGTTTATACTCATAACCTGTATTACCTGGCTCAAGTCTACCAGCACATGGAAATGTTCGAGAAG GCCGCTCATTATTGCCACAGCACACTGAAACGCCAGCTTGAACACAATGCCTACCACCCCATGGAGTGGG CCATCAACGCCGCTACTCTATCACAGTTCTACATCAATAAGCTCTGTTTTATGGAAGCCAGACATTGTCT GTCAGCTGCTAATGTTATTTTTGGTCAAACTGGAAAAATACCAGCTACAGAAGACACTCCTGAAGTTGAA GGAGACGTGCCAGAGCTTTACCATCAGAGGAAAGGGGAAATAGCAAGATGCTGGATCAAGTACTGCCTGA CTCTGATGCAAAACGCGCAGCTCTCCATGCAGGACAACATAGGAGAGCTTGACCTGGACAAGCAGTCGGA GCTCAGAGCTCTACGCAAGAAGGAGCTAGATGAGGAGGAGAGCGTCAGGAAAAGGGCTGTGCAGTTTGGA ACTGGGGAGCTGTGCGACGCCATCTCTGCAGTGGAGGAGAAAGTGAGATACTTGAGGCCCTTAGATTTTG AAGAAGCCAGGGAGCTTTTCCTGCTGGGTCAGCACTATGTGTGTGAGGCAAAGGAGTTCTTCCAGATCGA TGGGTATGTCACTGACCACATTGAAGTTGTCCAGGACCACAGCGCTCTGTTCAAGGTGCTCTCGTTCTTT GAGGCTGACATGGAGAGACGGTGCAAGATGCACAAGCGCAGGATAGCCATGCTAGAGCCCCTGACTGTGG ACCTGAACCCACAGTACTACCTGTTGGTCAGCAGGCAGATTCAGTTTGAAATCGCACACGCGTACTATGA CATGATGGACTTGAAGGTCGCCATTGCTGACAAGCTCAGGGACCCCGACTCACACATTGTCAAAAAAATA AATAGCCTGAATAAGTCGGCACTCAAGCACTACCAGCTCTTCCTAGACTCCCTGAGAGACCCAAACAAAG TCTTTCCTGAGCACATCGGGGAAGACGTCCTCCGCCCGGCCATGTTAGCTAAATTCCGGGTAGCTCGTCT GTATGGCAAAATCATCACTGCAGATCCCAAGAAAGAGCTAGAGAATTTGGCAACATCTTTGGAACATTAC AAATTTATTGTTGATTACTGTGAAACTCACCCCGAGGCTGCCCAGGAAATAGAGGTTGAGTTAGAACTTA GTAAAGAGATGGTCAGTCTCCTCCCAACAAAAATGGAGAGGTTCAGAGCCAAGATGGCCCTGACCTAAGC CTTGGTCAAAGGAAAGAGACGTGCAGCTTGAAGCCACTCCCCTCCCCTATAGCAAATGAGGCCCAGCCCC ACAGTCACCTTCGCCAGGGCCAGAGGGATGCACTCTGGGCTCCAGAAGCAGGCAGGTACCCGAGTCTATG CTAGGACCTTAAGCACCATAAATTAATAATTTATACCTAATTATGTAAGTTGCCTTGCTAAAGTGACAGG TGTTTTGCATTTTCGATCGCTTGTTTTCCTGTTTAAGTATAAACATCTCCTGCCTAGAGTTCTAAATGTT AAGTCCTTGCTGACAGTACTTGTTAGATTTCATACTAGGAACAACACTGGGTAGTGTACCTGGTAGAAGA GCCTGTTGCTGTGTTGAATGTGAGAAAGTCACTTCAGATGTCATTCTTTGTAAAATACTTACTTTCTTAA ACTCCAAATAAATCTTTCCCTTTTGAAAATACTAAAAACTTAATAATGTTGTAAAATGGCTTAATTATAG GACTGAAGAACTTGCACATAATAAATTTACTTGTTTCAACTAGTAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6493547 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6493547 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14326 same embryo
	EMAGE:14327 same embryo
	EMAGE:14325 same embryo
	EMAGE:14323 same embryo
	EurExpress:euxassay_004152 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source