EMAGE mouse gene expression spatial database

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EMAGE:14325

Pfkfb2 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 ( MGI:107815)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004123. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004123_01	euxassay_004123_02	euxassay_004123_03	euxassay_004123_04

euxassay_004123_05	euxassay_004123_06	euxassay_004123_07	euxassay_004123_08	euxassay_004123_09

euxassay_004123_10	euxassay_004123_11	euxassay_004123_12	euxassay_004123_13	euxassay_004123_14

euxassay_004123_15	euxassay_004123_16	euxassay_004123_17	euxassay_004123_18	euxassay_004123_19

euxassay_004123_20	euxassay_004123_21	euxassay_004123_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14325_wholemount_strong.wlz

14325_wholemount_moderate.wlz

14325_wholemount_weak.wlz

14325_wholemount_possible.wlz

14325_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14325_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5636
Entity Detected:	Pfkfb2, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 ( MGI:107815)
Sequence:	sense strand is shown >T5636 GGACAGGTCATAGGGTGCCTCCTGAAGAACTACCATGTCTGAGAATAGTACATTTTCCCCAGAAGACTGC AACAGCAGCTATAAACCCCACGCCTCAAATCTGCGAAGGGCAGGGAAGACATGCTCATGGGCTTCCTATA TGACCAACTCCCCAACACTCATTGTTATGATTGGCTTGCCAGCCCGGGGTAAAACTTATGTGTCTAAGAA ACTAACACGCTACCTCAACTGGATTGGAGTACCCACCAAAGTGTTTAATCTTGGGGTATATCGACGTGAA GCAGTCAAGTCCTATCAGTCTTATGATTTCTTTCGACATGACAATGAAGAGGCTATGAAGATCCGCAAAC AGTGTGCTCTGGTGGCACTGGAAGATGTTAAAGCCTATTTTACTGAAGAGAGTGGGCAGATTGCGGTGTT TGATGCCACCAATACCACTCGGGAGAGGAGGGACATGATTTTGAACTTTGCCAAGCAGAATGCCTTCAAG GTATTTTTTGTAGAATCTGTCTGTGATGATCCTGATGTCATTGCTGCCAATATTCTGGAGGTAAAAGTGT CGAGCCCTGACTACCCCGAAAGGAATAGGGAGAATGTGATGGAGGACTTCCTGAAGAGAATTGAGTGCTA CAAGGTCACTTACCAGCCCCTTGACCCAGACAACTATGATAAGGATCTTTCTTTCATAAAGGTGATAAAT GTAGGCCAGAGATTCCTGGTCAACAGAGTTCAGGACTACATCCAGAGTAAGATTGTCTACTACCTGATGA ATATCCACGTCCATCCTCGCACCATCTACCTTTGCCGGCACGGCGAGAGCGAGTTCAACCTTTTGGGGAA GATTGGGGGTGACTCTGGCCTCTCCGTGCGAGGAAAGCAGTTTGCTCATGCTCTGAAGAAGTTTCTGGAG GAACAGGAGATCCAGGACCTTAAAGTGTGGACGAGCCAGTTGAAGAGGACAATTCAGACTGCTGAGTCTC TTGGGGTGACCTATGAGCAGTGGAAGATCCTCAATGAGATTGATGCTGGCGTGTGTGAGGAGATGACATA TTCAGAGATTGAGCAACGGTACCCAGAGGAATTTGCACTCCGAGATCAAGAGAAGTATCTGTACCGATAC CCTGGTGGGGAGTCCTACCAGGACCTGGTGCAGCGGCTGGAGCCTGTCATCATGGAGCTAGAGCGTCAAG GCAACATCCTCGTTATCTCTCACCAGGCTGTCATGCGCTGCCTCCTGGCCTACTTCTTGGATAAAGGCGC AGATGAGTTACCATACTTGAGGTGCCCCCTGCACATCATCTTCAAACTTACTCCTGTGGCCTATGGTTGC AAAGTGGAAACAATTACTCTGAATGTGGATGCTGTAGACACACATCGTGACAAGCCAACTCACAACTTCC CCAAGAGCCAAACCCCTGTAAGGATGAGAAGGAACAGCTTTACGCCTCTGTCCAGTTCGAACACAATAAG GCGTCCAAGAAATTACAGTGTTGGGAGCCGACCCCTCAAGCCCCTCAGCCCTCTCCGTGCTCTGGACATG CAAGAAGGGGCGGACCAGCCGAAGACCCAAGTCAGCATTCCGGTGGTGTAACTGTGTGTTTCCCTCCAGC TTTGGCCTCTTGCCCTTGCCACTAATCAACCAAGAAGTCATTTAACTTCCCCCCACTCCTGTCCCAACGT TAATCTGAAAACAGAATCTGAGCTCATGCGTGTGCAGAGCAGCCTCAGCCATTCTCTAGTGTAAACACGT CACCCGGGGGCAAGATGGCAAGTTGGGTCTTTAGGACATGTCCCCAGATGGGATGATCTGAAAGAAGAGG AGCCAAGCATGAGTCATAGACTTGGTTCTTCTGTTTCCTGATGTCTGACCCAGTCTGTGAAGTCTGTGTG TGTGTATGTGTGTGTATGTATGCATGTGTGTGTATGTATGTGTGTGTGTGTATGTATGTATGTGTGTGTA TGTATGTATGTATGTGTATGTATGTATGTGTGTGTATGTATGTATGTATGTGTGTGTATGTATGTATGTA TGTGTGTGTATGTGTATGTATGTGTGTGTATGTATGTATGTATGTATGTGTGTGTATGTATGTATGTGTG TGTTTGTGTATGTGTGTGGAAGGGGGGTTGTTGGGAGGGGATGTGGGATAGTGTGAGATGTAACAACCTA ATTGTCCTTCACATGTGTCCTTTAAGTCTATCCTTATATCCTTAACTTCCCTGTCCTATAGCTTCTCTTT GGGTTGTCTGGTGTGATGCATGGCAGTATGATGTCTTAAAGTAAGGGACTGTTGATAAGTATCTAAGCCC CATTGGTGCTGTGTGCACTCGGGAATGGATCTACACTGGGAGAGTCTTTTCCCTTCCCTTCTCCCTTCCT CTCCAGCCAGGTCCAGCAAGGGTCAGCCCAGGCTACAGAACATCCACAGAAAGCCCTGGAGTTTGCAAAT GGACATAGGGAAGTGGAGAATTTGCTGGCTGAGCATAGACACCCCTCAATGGCATCCCTCACTTTGCTCT CCTGATGTGAGGCTGAGGCCAGACCTCTCCGAGGAACTGGAACTACTAAAAAGCTTGGGATGCCAGCATC ACTGAGGCTAGAACAGGAAGTTAAGTGGGAACTTCTCATTTGAGGCTACCAAAAATGAGGCCTGGAAAAC CTTCACTACATTTCTTCCCCTCATGCCTAACAGGAATAGTACTACACTGTGCCTCAGAAAGTCTTCAACA GCCTGAACAAGTTAGTTCTTGTCTCTTTTGAACCAAGTTTGCAAATACCATTCTACTTAGGCATTGAGTA CTCTACCTATGGCTGACTGACTGCATCCTTAGCTGGAGATCTGCCTAATGTGTGAACACACTGTTTCAGA ATTGGTTTCTAGGTGAGGACGAGCTTCAGGGAACCCTCTTCTGCTCACCGGAAGCCGCTCTTCTCAGCTG ATGGAGGAGAGCAGGCTGAAGTGCAATATGAATGCTAGTGGTCACCTCTTGCAGTGTGGAAAATGAGACA GGTCACCAGGTAAATGGAGGGTGTCAGGAACTAAGCTCTCAAGTGCTCTTCTCTGGTGGCAGGAGAATTG TATCCGTGGCTCCCTTTTCATCTAGAGAGGTTTTTGTTGTTCCATGCTCTTTGGTGCAGAGTCTGGCTGC TAGACTTCCCCAAAGGGGACCAACTTTTACATTCTCTTATAGCTACTGTTTTCTCTCGGAGGCTTGTACC TCACGCACTGGACAGGACTGATTCAGTGCCTCCTGACTGAGGGGTTGGGAATGAAGGCTAGGCGGGGCCA TCTCTAGCCCATAAGGAAATAAAGGTGGCTTCTTGGAAATAAATTACACTGTTTTTTTTTTATTTTTATT TTTTTAATTTCAAAATGGGTAGAAGCAGACATGCCCCTTGTGTCTGTCTAATTGATGTGATCTCTGGAAA GCTGTGGCTGTAACTGCAGGAGCAGTGGTCCTATGCTGTCCTCTGGGTGCCTCTGTTCTAGCTGTCACTA CTGTGATCAGCTGGTCTCCCTCGACACGTGGTAGATGCAGCATAGCCTTGGGGCATCAGTGAGTGGGTGT CTCTTTCAGAAGAATCAGATTGTTGTTGGAAGACCACTGAGTGTCTGATGCATACACACTGTGACTATGA ATGCACCCCTACTGTGTTATTTCTGAAGAAGAGTGCAGAAGCCACAAAGTGTATTGTACCTTACCAGTAG AATTCAGTGTTACTGGTTTTTTCTCCATTTTATTTTTATTTCCTTTTTAACTGGAGCTGCACAGTTGTTG GGCAGAAAGATAAGTATCAAGAAAAGTCATAAAATGTGCTTTTTCTAAATAGTTCTTTTATACTGTAAAA TCCTGCAGACTGTTACCTAAGTAGGATGTGATGAACTGCTGGCGGTTCCGTGACTCCCAGGTTGCAGCTT ACTTTCTATGTATGCATGTCACCTGGAGAGGCTCCTTCCTCTGCCTAGGACAGGCGTTCTTCCCTCAGCT GATCGCAGCATACTATACTACAGGGCTTTTTTCTTGTGGTATAGGATGCTTTTGCATTGAAGTACACTCC TTTCTGATGATCAGATGGTATTTTTATGAAGCTATTTATTTTGTGTGTTTGCATGTATTTAATCAGAATG TAATTTCTGTTCTGTGTTCTGCTAGCAATGTAGGTGGCTTGAACCTTGAGCCTAATGTCCATAGTATTTC CTTAAGTTAAGTTTGTAGACCATAAATGTCTGTAGATGTCAAAGAACAGTAACTATGTTATGACTGTAGA TTTTTTAAATTTTTTTTCTCATTGATTTTATTATAAACCAAGAAATGTATTTCTCTGGAAAAAAGTTCTC TCTTCGATAATTAAAACCATGCTGAAATATCTCAGTAACATATACTGCTTTTGTAATAAAATAAAGTTAT TTTTTATTGAAAGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6493944 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6493944 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14326 same embryo
	EMAGE:14327 same embryo
	EMAGE:14323 same embryo
	EMAGE:14324 same embryo
	EurExpress:euxassay_004123 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source