EMAGE mouse gene expression spatial database

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EMAGE:14326

Ifit1 interferon-induced protein with tetratricopeptide repeats 1 ( MGI:99450)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004118. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004118_01	euxassay_004118_02	euxassay_004118_03	euxassay_004118_04

euxassay_004118_05	euxassay_004118_06	euxassay_004118_07	euxassay_004118_08	euxassay_004118_09

euxassay_004118_10	euxassay_004118_11	euxassay_004118_12	euxassay_004118_13	euxassay_004118_14

euxassay_004118_15	euxassay_004118_16	euxassay_004118_17	euxassay_004118_18	euxassay_004118_19

euxassay_004118_20	euxassay_004118_21	euxassay_004118_22	euxassay_004118_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14326_wholemount_strong.wlz

14326_wholemount_moderate.wlz

14326_wholemount_weak.wlz

14326_wholemount_possible.wlz

14326_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14326_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6156
Entity Detected:	Ifit1, interferon-induced protein with tetratricopeptide repeats 1 ( MGI:99450)
Sequence:	sense strand is shown >T6156 CCCACGCGTCCGGGCAGGTTTCTGAGGAGTTCTGCTCTGCTGAAAACCCAGAGAACAGCTACCACCTTTA CAGCAACCATGGGAGAGAATGCTGATGGTGACCAGGTCATGGAGAATCTGCTTCAGCTGAGATGTCACTT CACATGGAAGCTGCTATTTGAAAATAATGACATACCTGATTTGGAAGTGAGAATCTCAGAGCAGGTCCAG TTCCTTGACATCAAGAACCCATTGGGGATGCACAACCTCCTGGCCTACGTGAGGCACCTGAAAGGCCAGC AGGACGAAGCCCTGCAGAGCTTGAAAGAAGCTGAAGCCTTGATCCAGAGCGAGCAGCTGAGCAAGAGAAG CCTGGCGACCTGGGGCAACTGTGCCTGGCTGCATTACCACAGGGGCAGCTTGGCAGAAGCCCAGATCTAC CTGGACAAGGTGGAGAAGGTGTGCAAGGAATTTTCAAGTCCCTTCCGCTACAGGCTGGAGTGTGCTGAGA TGGACTGTGAGGAAGGCTGGGCCTTGCTGAAGTGTGGAGGAGGAAATTATAAACAAGCCATGGCCTGCTT TGCGAAGGCTCTGAAAGTGGAGCCAGAAAACCCTGAGTACAACACTGGCTATGCAGTCGTAGCCTATCGC CAAGATTTAGATGACAACTTTATTTCTCTAGAACCTTTGAGGAAGGCTGTCCGGTTAAATCCAGAAGATC CATACCTTAAAGTTCTCCTTGCCCTAAAGCTTCAGGATTTAGGAGAACATGTTGAAGCAGAAGCACACAT TGAAGAAGCCCTCAGCAGCACATCTTGCCAAAGCTATGTCATTCGCTATGCAGCCAAATATTTCCGTAGG AAACATCGCGTAGACAAAGCTCTTCATCTTCTAAACAGGGCCTTGCAGGCATCACCTTCCTCTGGCTACT TACATTATCAAAAAGGGCTCTGCTACAAGCAACAAATCTCCCAACTGAGGACATCCCGAAACAGGCAGCC CAGAAGGCAGGACAATGTGCAAGAATTGGCACAACAGGCCATTCATGAATTTCAAGAGACTTTGAAACTG AGGCCCACATTTGAGATGGCCTATGTTTGCATGGCTGAAGTGCAGGCAGAAATTCACCAGTATGAAGAAG CAGAGAGAAATTTCCAGAAGGCACTGAACAACAAGACCCTCGTGGCTCACATAGAGCAGGATATTCACCT CCGCTATGGCCGTTTCCTACAGTTTCATAAGCAGTCAGAAGACAAGGCAATCACCCTCTACTTAAAAGGT CTAAAAGTGGAAGAGAAGTCCTTTGCTTGGAGGAAACTACTGACTGCTTTGGAGAAAGTGGCTGAAAGAC GTGTTTGTCAGAATGTTCATCTTGTAGAGAGTACCAGCCTTCTTGGGCTAGTCTACAAACTGAAAGGACA AGAGAAAAATGCTCTGTTTTACTATGAGAAGGCACTGAGGCTCACTGGGGAAATGAACCCTGCATTCTGA ATGCAGCTCACCTCTGTGACGTTAATATACTCACAACCAAGTGTTCCAATGCTCCTTCCTGATTTCTTTT CCCAGGTCTGTTTTGTTGTTGTTGTTGTTCGTTTTCTTTCTGAAATGCCAAGTAGCAAGGTACATTTTCT CCTGTGACTTCTTTCTGGTCTTTCTGACCCTGTCTAGCAGGCAATTCCATCCCAAGGATACATACTCACT TAAAGATCACAACTGATTCCTGCTGTTTTGGACTCCTGTGAGCTGTAGAATCACACAACCTTTTTCCTTA TGTCTGTGCCTTTGGAACAGATATCTTCCACCCTTTGCTAATGTCCTGGACAAAAAAGTCTTTGCCAAAG AAGGTATTTTTTTTTTAATGTCACCGGAAATGCTTATTACCATACAATTTCAGAGCAAAACAAATGAGCA AAGAAGAAAGTTTGAAAGCAAAAGAGGTCACACATTACTGGAAAGATACTAAGAACCAAGAAGACTTTTG GCTTACTAGAGAATTAAAATGAAAACCCTCCTTAGGTAGTAAAGTCTGAAAATTACATAAATAACTTCTT TCAATATTTATATGCTAAAAGTATTCATTGAAAGTGACCAAAATAGTTAAAGCCAAAACCAACAAAAAAA AGAAAGAAAGAAAGAAAGAGAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAAAAAGAAAA GAAAAGAAAAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3496673 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3496673 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14327 same embryo
	EMAGE:14325 same embryo
	EMAGE:14323 same embryo
	EMAGE:14324 same embryo
	EurExpress:euxassay_004118 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source