EMAGE mouse gene expression spatial database

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EMAGE:14364

Wasf1 WASP family 1 ( MGI:1890563)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004192. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004192_01	euxassay_004192_02	euxassay_004192_03	euxassay_004192_04

euxassay_004192_05	euxassay_004192_06	euxassay_004192_07	euxassay_004192_08	euxassay_004192_09

euxassay_004192_10	euxassay_004192_11	euxassay_004192_12	euxassay_004192_13	euxassay_004192_14

euxassay_004192_15	euxassay_004192_16	euxassay_004192_17	euxassay_004192_18	euxassay_004192_19

euxassay_004192_20	euxassay_004192_21	euxassay_004192_22	euxassay_004192_23	euxassay_004192_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14364_wholemount_strong.wlz

14364_wholemount_moderate.wlz

14364_wholemount_weak.wlz

14364_wholemount_possible.wlz

14364_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14364_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 18 weak expression: see section 08

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 09 17 18 19 20 weak expression: see section 08

vagus x ganglion

moderate

regional

moderate expression: see section 09 weak expression: see section 17

spinal cord

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 14 15 16 17 18

lower jaw incisor

moderate

regional

moderate expression: see section 11 15 16 weak expression: see section 12

lower jaw molar

moderate

regional

moderate expression: see section 08 19

upper jaw incisor

moderate

regional

moderate expression: see section 11 15 16 weak expression: see section 12

upper jaw molar

moderate

regional

moderate expression: see section 08 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7897
Entity Detected:	Wasf1, WASP family 1 ( MGI:1890563)
Sequence:	sense strand is shown >T7897 GGACAGGGGGAAGCGCCGTCCTCTTGCCCACGGACGGGTGAGCTGGTTCAGTGGCTGAGCTCAAGATGCC GTTGGTGAAAAGAAACATCGACCCTCGGCACTTGTGCCACACAGCACTGCCCAGGGGCATTAAGAACGAA CTGGAATGTGTAACCAACATTTCCCTGGCAAATATAATTAGACAACTGAGTAGCCTAAGTAAGTATGCTG AAGACATATTTGGAGAATTATTCAATGAAGCACACAGTTTTTCCTTCAGAGTTAACTCGTTGCAAGAGCG CGTGGACCGATTGTCTGTTAGTGTCACACAGCTTGATCCTAAGGAGGAAGAACTGTCTTTGCAAGATATA ACGATGAGAAAGGCTTTCCGGAGCTCCACAATTCAAGATCAGCAGCTTTTCGACCGCAAGACGTTGCCTA TTCCATTGCAAGAAACATACGATGTGTGTGAGCAACCTCCGCCTCTCAATATCCTCACCCCTTACAGGGA TGATGGTAAGGAAGGGCTGAAGTTTTATACCAATCCTTCATATTTCTTTGATCTGTGGAAAGAAAAGATG TTGCAAGATACAGAGGACAAGAGGAAGGAAAAGAGAAAGCAGAAGCAGAAAAATCTAGACCGTCCTCATG AACCAGAGAAAGTGCCAAGAGCACCTCATGACAGGCGGCGGGAATGGCAGAAGCTGGCCCAAGGTCCAGA GCTGGCTGAGGATGACGCTGACCTCCTACACAAGCATATTGAAGTTGCCAATGGCCCAGCCTCTCATTAT GAGACAAGGCCACAGACATACGTGGATCATATGGACGGATCGTACTCACTCTCTGCCTTGCCATTCAGTC AGATGAGTGAACTTCTGACCAGAGCTGAGGAGAGGGTCTTAGTCAGACCCCATGAACCTCCTCCGCCTCC ACCAATGCATGGAGCAGGAGATGCAAAGCCCACACCCACCTGTATCAGTTCTGCTACAGGTTTGATAGAG AATCGCCCCCAGTCCCCAGCTGCAGGCAGAACACCTGTGTTTGTGAGCCCCACTCCCCCACCTCCTCCCC CACCTCTTCCGTCTGCCTTGTCCACTTCTTCACTAAGAGCTTCAATGACCTCAACTCCTCCCCCACCAGT CCCTCCTCCGCCTCCTCCCCCAGCCACTGCTTTGCAGGCCCCAGCAGTGCCCCCACCTCCGGCTCCTCTT CAGATTGCCCCTGGTGTTCTTCACCCAGCTCCTCCTCCAATTGCTCCTCCTCTAGTGCAGCCCTCTCCCC CAGTAGCTCGAGCTGCCCCAGTATGCGAGACTGTGCCAGTTCACCCACTCCCGCAAGGTGAAGTGCAGGG GCTGCCTCCACCCCCACCACCGCCTCCTCTACCTCCGCCTGGCATCCGGCCATCATCTCCTGTCGCAGTT GCAGCTCTTGCTCATCCTCCCTCTGGGCTCCATCCAGCACCATCTACTGCCCCGGGTCCCCATGCTCCAT TAATGCCTCCATCTCCTCCATCACAAGTTCTACCTGCCTCTGAGCCAAAGCGCCATCCATCCACCCTGCC TGTAATCAGTGACGCAAGGAGTGTGCTGCTGGAAGCCATCCGAAAAGGTATTCAGCTTCGCAAAGTGGAA GAGCAGCGTGAACAGGAAGCAAAACACGAGCGGATTGAAAACGACGTGGCCACCATCCTGTCTCGCCGCA TTGCTGTGGAGTATAGCGACTCGGAAGATGACTCGGAGTTCGATGAAGTAGACTGGCTGGAGTAAGAAAG CCACAGTGACTTGGAAGATGACTCGGAGTTCGATGAAGTAGACTGGATGGAGTAAGACAGCCGCCTTGCT CTGTATTGCAAGGCTGAATGCACATGTCCCTCGTGGCGCTTGTTCTTTGAAAATGTTCGGTCATTCTAGT GTTTTGCTTCCTATTCCTCATAATGAATAACCCTTTTCCTCCATTATTTTTGATTTCTATGAAAAGTATT AGCATATATTTCAAACTAAATATTTTGTGGCTTACCTTAATTTTTCCCCCTGGAAAAATATAATTTGGTC CAATAAACTACTAAGTATTAAGCATGGAAAGCCGTTATTAGAGTAGCAGATTCAGTTTTTGATATTCCTT AATTGTGCACTTTGTGATTTTTTTTTTTTTTTTTTAAATTTGAAGAACGCAACTGAGACTGGAATCTTGA AGGCAGCTGTGTCTGGGAACGAGCCTTATTCCGTTTCTTGATGTTTTAAATGAAGAAACACTGCCTCGAT TATATGAATACACTCAGAAGGAACATGTAGCTTGTAGTGTTGAATTCTCTTAAAGGAATGGTTAGCTTTG GTTATCATTTTGTTGTTTTTGAATACTTGCCTTATTTGAACGTTTAGCAGAACCCCTTCCCACTTATATA TTGTGTGGTACAGTTTTGCTTGCCTATAGAAGTTTAAATTTTTCCATGTGAAATACTCAGAATAAGCATA TGTGTGACTTACATAATGTGAGAATTACAATCTGATGTAATAAACGGTTCATTTTAAAGGAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6509953 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6509953 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14368 same embryo
	EMAGE:14365 same embryo
	EMAGE:14367 same embryo
	EMAGE:14363 same embryo
	EMAGE:14366 same embryo
	EurExpress:euxassay_004192 same experiment
	MGI:4829186 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source