EMAGE mouse gene expression spatial database

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EMAGE:14366

Adamts1 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 1 ( MGI:109249)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004197_01	euxassay_004197_02	euxassay_004197_03	euxassay_004197_04	euxassay_004197_05

euxassay_004197_06	euxassay_004197_07	euxassay_004197_08	euxassay_004197_09	euxassay_004197_10

euxassay_004197_11	euxassay_004197_12	euxassay_004197_13	euxassay_004197_14	euxassay_004197_15

euxassay_004197_16	euxassay_004197_17	euxassay_004197_18	euxassay_004197_19	euxassay_004197_20

euxassay_004197_21	euxassay_004197_22	euxassay_004197_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14366_wholemount_strong.wlz

14366_wholemount_moderate.wlz

14366_wholemount_weak.wlz

14366_wholemount_possible.wlz

14366_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14366_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 02 03 04 05 06 17 18 19 20 21 22

mandible

moderate

regional

moderate expression: see section 04 05 08 09 10 11 15 16 17 18 19 20 21 22 weak expression: see section 06 07

maxilla

moderate

regional

moderate expression: see section 08 09 10 11 15 16 17 18 19 20 21 weak expression: see section 06 07

cranium

moderate

regional

moderate expression: see section 01 02 03 04 05 20 21 22 23

clavicle

strong

regional

strong expression: see section 08 09 10 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7873
Entity Detected:	Adamts1, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 1 ( MGI:109249)
Sequence:	sense strand is shown >T7873 GCAGCTCCGAGCTAGGTGCTATCGCAAGGCCAGAGCGCACAGCCCGGCGGAGAGAGCAGATCCTTGCTCA GATCGAGTCAAATCGGGGCCAAGGCGGAGGACGAAGAGTCCAGGCTCCTATTCTGGACTTGTTCCCCAGC TCCGGGGGCGCTTCTAGGTCCTGCAGCAGCCAGGAGTGCGGAGCCACCAACTCGGTGCTGGAATGAAAAA ATTCCCGCGCGCCAGTGCAGAATCTTTCTAAGTGACCCGGAGCTTCGGGTGCTAGCTCTGCACGAACTTT CCCATCAAAGTGATCGTGAATTTTAAGCATCAGGAGCAGGCCAGCGAAGCTCTACGCGTCTAAACGTCTA TCCAGACCAAGAGTTCTCTGCGGTGCAGGGTGCGGTGCCATGCAGCCAAAAGTCCCTTTGGGGTCACGCA AGCAGAAGCCCTGCTCCGACATGGGGGACGTCCAGCGGGCAGCGAGATCTCGGGGCTCTCTGTCCGCACA CATGCTGTTGCTGCTCCTCGCTTCCATAACAATGCTGCTATGTGCGCGGGGCGCACACGGGCGCCCCACG GAGGAAGATGAGGAGCTGGTCCTGCCCTCGCTGGAGCGCGCCCCGGGCCACGATTCCACCACCACACGCC TTCGTCTGGACGCCTTTGGCCAGCAGCTACATCTGAAGTTGCAGCCGGACAGCGGTTTCTTGGCGCCTGG CTTCACCCTGCAGACTGTGGGGCGCAGTCCCGGGTCCGAGGCACAACATCTGGACCCCACCGGGGACCTG GCTCACTGCTTCTACTCTGGCACGGTGAACGGTGATCCCGGCTCTGCCGCAGCCCTCAGCCTCTGTGAAG GTGTGCGTGGTGCCTTCTACCTACAAGGAGAGGAGTTCTTCATTCAGCCAGCGCCTGGAGTGGCCACCGA GCGCCTGGCCCCTGCCGTGCCCGAGGAGGAGTCATCCGCACGGCCGCAGTTCCACATCCTGAGGCGAAGG CGGCGGGGCAGTGGCGGCGCCAAGTGCGGCGTCATGGACGACGAGACCCTGCCAACCAGCGACTCGCGAC CCGAGAGCCAGAACACCCGGAACCAGTGGCCTGTGCGGGACCCCACGCCTCAGGACGCGGGAAAGCCATC AGGACCAGGAAGCATAAGGAAGAAGCGATTTGTGTCCAGCCCCCGTTATGTGGAAACCATGCTCGTGGCT GACCAGTCCATGGCCGACTTCCACGGCAGCGGTCTAAAGCATTACCTTCTAACCCTGTTCTCGGTGGCAG CCAGGTTTTACAAGCATCCCAGCATTAGGAATTCAATTAGCCTGGTGGTGGTGAAGATCTTGGTCATATA TGAGGAGCAGAAGGGACCAGAAGTTACCTCCAATGCAGCTCTCACCCTTCGGAATTTCTGCAACTGGCAG AAACAACACAACAGCCCCAGTGACCGGGATCCAGAGCACTATGACACTGCAATTCTGTTCACCAGACAGG ATTTATGTGGCTCCCACACGTGTGACACTCTCGGGATGGCAGATGTTGGAACTGTATGTGACCCCAGCAG GAGCTGCTCAGTCATAGAAGATGATGGTTTGCAAGCCGCCTTCACCACAGCCCACGAATTGGGCCATGTG TTTAACATGCCGCACGATGATGCTAAGCACTGTGCCAGCTTGAATGGTGTGACTGGCGATTCTCATCTGA TGGCCTCGATGCTCTCCAGCTTAGACCATAGCCAGCCCTGGTCACCTTGCAGTGCCTACATGGTCACGTC CTTCCTAGATAATGGACACGGGGAATGTTTGATGGACAAGCCCCAGAATCCAATCAAGCTCCCTTCTGAT CTTCCCGGTACCTTGTACGATGCCAACCGCCAGTGTCAGTTTACATTCGGAGAGGAATCCAAGCACTGCC CTGATGCAGCCAGCACATGTACTACCCTGTGGTGCACTGGCACCTCCGGTGGCTTACTGGTGTGCCAAAC AAAACACTTCCCTTGGGCAGATGGCACCAGCTGTGGAGAAGGGAAGTGGTGTGTCAGTGGCAAGTGCGTG AACAAGACAGACATGAAGCATTTTGCTACTCCTGTTCATGGAAGCTGGGGACCATGGGGACCGTGGGGAG ACTGCTCAAGAACCTGTGGTGGTGGAGTTCAATACACAATGAGAGAATGTGACAACCCAGTCCCAAAGAA CGGAGGGAAGTACTGTGAAGGCAAACGAGTCCGCTACAGGTCCTGTAACATCGAGGACTGTCCAGACAAT AACGGAAAAACGTTCAGAGAGGAGCAGTGCGAGGCGCACAATGAGTTTTCCAAAGCTTCCTTTGGGAATG AGCCCACTGTAGAGTGGACACCCAAGTACGCCGGCGTCTCGCCAAAGGACAGGTGCAAGCTCACCTGTGA AGCCAAAGGCATTGGCTACTTTTTCGTCTTACAGCCCAAGGTTGTAGATGGCACTCCCTGTAGTCCAGAC TCTACCTCTGTCTGTGTGCAAGGGCAGTGTGTGAAAGCTGGCTGTGATCGCATCATAGACTCCAAAAAGA AGTTTGATAAGTGTGGCGTTTGTGGAGGAAACGGTTCCACATGCAAGAAGATGTCAGGAATAGTCACTAG TACAAGACCTGGGTATCATGACATTGTCACAATTCCTGCTGGAGCCACCAACATTGAAGTGAAACATCGG AATCAAAGGGGGTCCAGAAACAATGGCAGCTTTCTGGCTATTAGAGCCGCTGATGGTACCTATATTCTGA ATGGAAACTTCACTCTGTCCACACTAGAGCAAGACCTCACCTACAAAGGTACTGTCTTAAGGTACAGTGG TTCCTCGGCTGCGCTGGAGAGAATCCGCAGCTTTAGTCCACTCAAAGAACCCTTAACCATCCAGGTTCTT ATGGTAGGCCATGCTCTCCGACCCAAAATTAAATTCACCTACTTTATGAAGAAGAAGACAGAGTCATTCA ACGCCATTCCCACATTTTCTGAGTGGGTGATTGAAGAGTGGGGGGAGTGCTCCAAGACATGCGGCTCAGG TTGGCAGAGAAGAGTAGTGCAGTGCAGAGACATTAATGGACACCCTGCTTCCGAATGTGCAAAGGAAGTG AAGCCAGCCAGTACCAGACCTTGTGCAGACCTTCCTTGCCCACACTGGCAGGTGGGGGATTGGTCACCAT GTTCCAAAACTTGCGGGAAGGGTTACAAGAAGAGAACCTTGAAATGTGTGTCCCACGATGGGGGCGTGTT ATCAAATGAGAGCTGTGATCCTTTGAAGAAGCCAAAGCATTACATTGACTTTTGCACACTGACACAGTGC AGTTAAGAGGCGTTAGAGGACAAGGTAGCGTGGGGAGGGCTGATACACTGAGTGCTGGAGGGATCCAGTG AGTCAAACCAGTAAGCAGTGAGGTGTGGCAAGGAGGTGTGTGTAGGGGATACATAGCAAAGGAGGTAGAT CAGGACACTACCCTGCCAGTTACATTCTGATAAGGTAGTTAATGAGGCACAGTAGCATCTGAAAGACCAT ACAGAGCACTAAGGAGCCCCAAAGCACTATTAGTATCTCTTTTCTTATATCTATCGCCCAAATAATTTTC AGAGTCTGGCAGAAGCCCTGTTGCACTGTACTGACTAGATACTTCTTATCACAAAGATTGGGAAAGGCAA AGCAGAAAGATGGTAAGACTGGGTTTCAAACAAGGCTTGGTTTCTATCACTGGAGGCAAGGAGGAGGGGA CAAACAAGATCATTATTCGAAGTCGCTGGTTGCTGTGGTTTTACGGAAGGTTGATGCATCATTCCTATCA ACAGTGAAAAGTTCAGCTTGTTCAACGTGACAGAAAGGCTCATCTCCGTGAAAGAGCTCCTGATTTCTTC TTACACCATCTCAGTTCTTAACTATAATTCATGTTGAGGTAGAAACAATTCATCTATTTATAAAATGTAC ATTGGAAAAAAAAAAGTGAAGTTTATGAGGTACACATAAAAACTGAAGGAAACAATGAGCAACATGCCTC CTGCTTTGCTTCCTCCTGAGGTAAACCTGCCTGGCGATTGAGGTTGTTTAAGATTATCCATGGCTCACAA GAGGCAGTAAAATAATACATGTTGTGCCAGAGTTAGAATGGGGTATAGAGATCAGGGTCCCATGAGATGG GGAACATGGTGATCACTCATCTCACATGGGAGGCTGCTGCAGGGTAGCAGGTCCACTCCTGGCAGCTGGT CCAACAGTCGTATCCTGGTGAATGTCTGTTCAGCTCTTCTACTGAGAGAGAATATGACTGTTTCCATATG TATATGTATATAGTAAAATATGTTACTATGAATTGCATGTACTTTATAAGTATTGGTGTGTCTGTTCCTT CTAAGAAGGACTATAGTTTATAATAAATGCCTATAATAACATATTTATTTTTATACATTTATTTCTAATG ATAAAACCTTTAAGTTATATCGCTTTTGTAAAAGTGCATATAAAAATAGAGTATTTATACAATATATGTT AACTAGAAATAATAAAAGAACACTTTTGAATGTGTATGCCTATTTTCTGGAGTGGGATTAACTTCTGGGC AAGAAATCTGATGAGACACAAACATTGGACTTCAAGACAGTTTTAAATTTTGGGTAAATGAACTGTATTT CCTGTTTATAGACGTACTAATAAAAAAGAAGTTGATGATGTCTTTAGTGGTAAGATTGTTACTAATGTGG TTGGCAAATTGCTGTAAAGAGCCAGATAGTAAGCATTTATGGCATTGTAGGCTATCTTTCCTGCCACAAC CATGTGACAGTGAGTGCTTTGTAGGACTGAGAGCAGCCATAAATGACATGTAAATGATAAACTGTGGCTG TGCTTTAATAAAACTTTATTTACAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6505494 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6505494 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14368 same embryo
	EMAGE:14365 same embryo
	EMAGE:14367 same embryo
	EMAGE:14363 same embryo
	EMAGE:14364 same embryo
	EurExpress:euxassay_004197 same experiment
	MGI:4822981 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source