EMAGE mouse gene expression spatial database

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EMAGE:14433

Rmnd5a required for meiotic nuclear division 5 homolog A (S. cerevisiae) ( MGI:1915727)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004222. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004222_01	euxassay_004222_02	euxassay_004222_03	euxassay_004222_04

euxassay_004222_05	euxassay_004222_06	euxassay_004222_07	euxassay_004222_08	euxassay_004222_09

euxassay_004222_10	euxassay_004222_11	euxassay_004222_12	euxassay_004222_13	euxassay_004222_14

euxassay_004222_15	euxassay_004222_16	euxassay_004222_17	euxassay_004222_18	euxassay_004222_19

euxassay_004222_20	euxassay_004222_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14433_wholemount_strong.wlz

14433_wholemount_moderate.wlz

14433_wholemount_weak.wlz

14433_wholemount_possible.wlz

14433_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14433_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7909
Entity Detected:	Rmnd5a, required for meiotic nuclear division 5 homolog A (S. cerevisiae) ( MGI:1915727)
Sequence:	sense strand is shown >T7909 CCCGGGAGAACCAGGTCATCGGCCGGTTCCCGTGAAAACAAAAACAATCGGCGGCGCCGCCGAGGGGCGG CCGAACGCGGCGAGCCAGGGGGGCCCGGGGCGAGACGCGGCGCCGGGTAGGGCGGGCGAGCGAGCGCGAG CGGCGGGGCCGGGGCGCCATGGGGCGGGAGGGCACAGGCTGCGCGGGGCTCCCCCGGCGCCGCGGCTAGC GCGCCCGTCTTGCCTTCGCCTCCCGCGCCGCCCGCTCGCCGCCTTGGGGGAGGAGGAGCAGGACGCGGCC TCGGTGGGGCCCGGGCCGAACCACTGCGGACCCCCGGGCGCCGGGGAGCCGAGCGCCGCGGTCTCCGGCA TGGATCAGTGCGTGACGGTGGAGCGCGAGCTGGAGAAGGTGCTGCACAAGTTCTCGGGCTACGGGCAGCT GTGCGAGCGGGGCCTGGAGGAGCTCATCGACTACACCGGCGGCCTCAAGCACGAGATCCTGCAGAGCCAC GGTCAAGATGCTGAATTATCAGGGACACTTTCACTTGTTCTGACACAGTGCTGCAAAAGAATAAAGGACA CTGTCCAGAAGTTGGCCTCTGACCACAAAGACATCCATAGCAGTGTTTCTCGAGTTGGAAAAGCCATTGA TAAGAATTTTGATTCTGACATTAGCAGTGTGGGAATAGATGGCTGCTGGCAGGCAGACAGCCAACGGCTT CTCAATGAGGTCATGGTGGAACACTTCTTCCGACAAGGAATGCTGGATGTAGCTGAGGAGCTTTGCCAGG AATCTGGTCTGTCTGTAGACCCAAGTCAGAAAGAACCATTTGTGGAGTTAAATAGAATATTAGAAGCATT AAAAGTCAGAGTTCTAAGGCCTGCTCTCGAGTGGGCAGTATCAAACCGAGAAATGCTCATAGCCCAAAAC AGTTCCTTGGAATTTAAGCTACACCGACTGTATTTTATTAGCTTGTTAATGGGTGGAACTACAAATCAGC GAGAAGCCTTACAATATGCGAAAAATTTCCAGCCATTTGCCCTAAATCATCAAAAAGACATTCAGGTATT GATGGGAAGCCTCGTGTACCTGCGACAAGGGATTGAAAACTCACCATATGTTCACTTACTTGATGCAAAC CAGTGGGCTGACATCTGTGACATCTTTACACGGGATGCTTGTGCCCTCCTGGGGCTCTCCGTGGAGTCTC CTCTCAGTGTCAGTTTCTCAGCAGGTTGTGTGGCGCTGCCTGCGCTAATTAACATCAAAGCTGTGATTGA GCAGAGGCAGTGTACTGGAGTTTGGAACCAGAAAGACGAATTACCTATTGAAGTGGACCTTGGTAAAAAG TGCTGGTATCACTCCATATTTGCCTGTCCCATTCTTCGTCAGCAAACAACAGATAACAATCCACCCATGA AATTGGTTTGTGGTCATATTATATCAAGAGACGCCCTGAATAAAATGTTTAATGGTAGCAAATTAAAATG TCCATATTGCTCAATGGAACAGAGTCCAGGAGATGCCAAACAGATATTTTTCTGAAGAATGAGTTTGTTT GCAATTTGTAAGTGAAACTGAATTATGGGTACATTCAAGACAAGAGTGTTCCATTGACTGCAGCTATCCA AGGACCGCCTGTTCATAAGCTATGCTCCAGAGGCTGCCGATTCACTCGTGTGCACGGAGGGGGTGCTCCA GATGGGAATCACACAGGGCTTTCTTCACTCTTGGTCTTCGTTTCTGATCAAGTAAACACCAGCAGTTGTC ATTCAGTGCAGGTTTTTGTACTTCTATATGGTGATTTTTTTACTTAAAAGCAGAAACAGAAGTTGACCTT CCTGACATGTGTTTAATATTCCTCCTGCTTTTACAGATTCTGACGTTTTCTTGATAATTGTAAGCTTGAG AGTGTTTGTGGAAGAACTTACTTTCTTCTTATGTATACATAATTAAATGAAAAGTCTTCATAGGAGTTTG ACAAAATGAATTGTGGTTATAAAACGAATTTGCTTTTTTGTTGTTTTGTTTTTCTTTTTTGGCCTAAGGA AGAAAGCTGTGATAAATTTCAAATTTGCATAGCTTTTCAATGTTTTGCTCTGCTCCCGCTCTTGCTTCAG AGTCGGCACACTCACCTGCATTTGAGTTCTGTCTATAGCCCAGCAGGCTGCCTGTTTAAAATCCCATCAT GAATTTAACAGGCTGATGTGAGAATGAAATAGATATTACTGGGGTTTTGTTGTTGTTTGTTTATTTGTTT TGCTTTTATTACCAAGAGGTGCTTTTTAATAAATGGATATTGAAGTTAGGGTGTTACTAATTTGATGTAT GGTTTCACAGTCTAGCATACTGTCCTTTGACATCTGCCTTTAAGACTTGGCTGAGTGTCTCATTAGTTTA TCATCACAGATACGCAGTGTTATGCATGTGTATAGAAGTGTGTGCACCAGCATCAAACATTGTGTGTGTG GAAGGGAAGAAGCCTGTCCATTCTAAACGCAGTTGCCAGTCTCATCACTTCAGGTCCTTACGGGCAGGCT CTAGCAACTTTCCGTGTATGGACCTCGTTTTTTGCTGTTTTGTGTTTAATTAGTATATTGTTCATGCCTC TCTTCTGCAGTGTCTCATCTCATAGACTGTGAACCTGTATATTATTCAAATGGCTACAGATAATGCTCTT TTCTTTTGTGAGGTCTCTTCATTTAATGCACTGCCCAGAAAGAGCCATGTGTAAGAGTTGTTCTCTGTTT GAGGAACTAACTACATGGAAAAGACTTCTGACTTAAACCCATGAAATACTTCATCTTGAGAAGAGTGCTA TGTGGAAATCACCAAATATCTCGCAACTTTATTTCATCTGGTGTAAATCTGAACATCAACATAGGAAAAC TGTCATGAGAAAATGAAAAAGCATAAACACAGAAGCAACGAGAAATGTGACTCTTGTTATTTTAAACCAC AGACGGACTTGGGTTAAGGGAATGGGGACGACAGCTTTGGTGCTAAGTTAATCAGAAATTGCGAGCATGC ACAGTGGTATGCCAGCCTGGGTGATGCTTTCCTAGGAGAGCCGGTATTTGCTTGTAAGGGAAAGAATGGT ATTGTAGAAAAACCCAAGAAATGACCACGTGGTCAGTTTCATGGTGATGGCTACTGTGTACTGTGTAGGT TACCTGGGTCAAGACTGGTCCAGCTTAGAGTGCATGCTCTGTTCATACTCAGCCTAGCTCACAGTGACTT GTGCTTCACTGTCAGGATGCTCTGTAAAGTTCCTCATCCCTGTGAAGCAGGGAAAGAACAGAGGTGCCTC TGGACTGCAGGAGAAAGGCGTCGTGCCAGGCAGTATCCTGTGGAGGGAGGAGGGTGTACGTTCGTTTACT ATGGATAGTTTTTCCTTTGAATTAAATTGTAGCGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:6512227 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6512227 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14432 same embryo
	EMAGE:14430 same embryo
	EMAGE:14435 same embryo
	EMAGE:14434 same embryo
	EMAGE:14431 same embryo
	EurExpress:euxassay_004222 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source