EMAGE mouse gene expression spatial database

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EMAGE:14434

Wnt6 wingless-related MMTV integration site 6 ( MGI:98960)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004236. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004236_01	euxassay_004236_02	euxassay_004236_03	euxassay_004236_04

euxassay_004236_05	euxassay_004236_06	euxassay_004236_07	euxassay_004236_08	euxassay_004236_09

euxassay_004236_10	euxassay_004236_11	euxassay_004236_12	euxassay_004236_13	euxassay_004236_14

euxassay_004236_15	euxassay_004236_16	euxassay_004236_17	euxassay_004236_18	euxassay_004236_19

euxassay_004236_20	euxassay_004236_21	euxassay_004236_22	euxassay_004236_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14434_wholemount_strong.wlz

14434_wholemount_moderate.wlz

14434_wholemount_weak.wlz

14434_wholemount_possible.wlz

14434_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14434_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

ear

moderate

regional

moderate expression: see section 01

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03

cornea

moderate

regional

moderate expression: see section 05 weak expression: see section 02 03 04

nasal cavity respiratory epithelium

weak

regional

weak expression: see section 15 17 18 19

hindgut

weak

regional

weak expression: see section 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7905
Entity Detected:	Wnt6, wingless-related MMTV integration site 6 ( MGI:98960)
Sequence:	sense strand is shown >T7905 CCGCCGCGCCCTCCTCGCCCGGGATGGGCCCCCCCGCCGCCACCGCCGCCGGAGCCCTAGTCTCCGGGCC GCCGCCTCGGTCGCCGCGTTTGCCCTGAAGCCCGGTGCCCGCGCGCCCCGGCTCACCCCGCAGCTTCACT CCCCACCCCCAGCCGCCTCCCCGGCCAGACTGCGGTAGAGCTCTCAGGATGCTGCCGCCGGTGCCCTCCC GCCTCGGACTGCTGCTGCTGCTCTTGTGCCCCGCGCACGTCGATGGACTGTGGTGGGCCGTGGGCAGCCC CTTGGTCATGGATCCTACCAGCATCTGCAGGAAGGCCAGGCGGCTGGCAGGAAGACAGGCCGAGCTGTGC CAGGCGGAGCCGGAAGTAGTGGCAGAGCTTGCCCGAGGCGCAAGACTGGGGGTTCGAGAATGTCAGTTCC AGTTCCGTTTCCGACGCTGGAACTGCTCCAGCCACAGCAAGGCCTTTGGGCGCGTCCTGCAGCAGGACAT CCGAGAGACAGCTTTCGTGTTTGCAATCACCGCAGCTGGTGCCAGCCACGCGGTCACTCAAGCCTGTTCC ATGGGAGAGCTCCTACAGTGTGGTTGTCAGGCACCCCGCGGGCGGGCACCGCCTAGGCCCTCCGGCCTTC TGGGCACTCCTGGACCTCCAGGACCAACTGGCTCTCCAGATGCTAGCGCAGCCTGGGAGTGGGGAGGCTG CGGAGACGATGTGGACTTCGGGGATGAGAAGTCAAGACTCTTTATGGATGCGCAGCACAAGCGGGGCCGT GGAGATATCCGTGCATTGGTGCAACTGCACAACAACGAGGCGGGCAGGCTGGCGGTGCGGAGTCACACGC GCACCGAGTGTAAGTGCCATGGGCTTTCGGGTTCCTGCGCTCTGCGCACCTGCTGGCAGAAGCTGCCTCC GTTCCGCGAGGTGGGCGCACGGCTGCTGGAGCGCTTCCACGGCGCCTCGCGCGTCATGGGCACCAACGAC GGCAAAGCTCTGCTGCCTGCGGTCCGCACACTCAAGCCTCCCGGACGAGCGGATCTCCTCTACGCAGCCG ATTCACCCGACTTCTGCGCCCCCAACCGGCGCACGGGTTCGCCGGGCACGCGCGGACGCGCCTGCAACAG CAGTGCCCCGGACCTCAGCGGCTGCGACCTGTTGTGCTGCGGTCGCGGGCACCGCCAGGAGAGCGTACAG CTCGAGGAGAACTGTCTGTGCCGCTTCCACTGGTGCTGCGTGGTGCAATGCCACCGCTGCCGGGTGCGCA AGGAACTCAGCCTGTGCCTCTGACCCGTCGCCTGCCTCGGAACTGCTGGCAGCCACCTCTGGGCCATCTA CAGGACTATTAGATTCCAGCAGGGGGCGCTGTCTGAGTCCAGCAGCTCCCTAGGAAAAGTACCTATCCAG GCCTTGGGAAATTACAGGGGCCAGCCAGGAACTTGGGGTTTACACCAGCCCACGAAAGCCCGGGGGAACA TACCCCTCCAGCATTCCCCTGAAAGGCCCTTTGCTAGTTCCTGCAGGAGATCACTCCCCTTGGCCCCCCA GATGGAAATAAGAAAGCCAGACTCTGCCCTCTGGAAATAATATTCCTCAGAATTACTGGGATGGATGGGT GAGTTTAGTATCAATAAAGACATTTAAATCCACAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6511061 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6511061 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14433 same embryo
	EMAGE:14432 same embryo
	EMAGE:14430 same embryo
	EMAGE:14435 same embryo
	EMAGE:14431 same embryo
	EurExpress:euxassay_004236 same experiment
	MGI:4829226 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source