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EMAGE:14467

Fam46b family with sequence similarity 46, member B ( MGI:2140500)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14467 EMAGE:14467 EMAGE:14467 EMAGE:14467 EMAGE:14467
"Pseudo-wholemount" of euxassay_004295. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004295_01 euxassay_004295_02 euxassay_004295_03 euxassay_004295_04
EMAGE:14467 EMAGE:14467 EMAGE:14467 EMAGE:14467 EMAGE:14467
euxassay_004295_05 euxassay_004295_06 euxassay_004295_07 euxassay_004295_08 euxassay_004295_09
EMAGE:14467 EMAGE:14467 EMAGE:14467 EMAGE:14467 EMAGE:14467
euxassay_004295_10 euxassay_004295_11 euxassay_004295_12 euxassay_004295_13 euxassay_004295_14
EMAGE:14467 EMAGE:14467 EMAGE:14467 EMAGE:14467 EMAGE:14467
euxassay_004295_15 euxassay_004295_16 euxassay_004295_17 euxassay_004295_18 euxassay_004295_19
EMAGE:14467 EMAGE:14467
euxassay_004295_20 euxassay_004295_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14467Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14467_wholemount_strong.wlz
14467_wholemount_moderate.wlz
14467_wholemount_weak.wlz
14467_wholemount_possible.wlz
14467_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14467_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4384
Entity Detected:Fam46b, family with sequence similarity 46, member B ( MGI:2140500)
Sequence:sense strand is shown

>T4384
CCACGCGTCCGCTCGCCGCCGCCGGGTCCCCGCACCCCGCCTTGGCCCGTGCACAGCCACTCTCCCTGCC
CTCCTCGCCTTCACCATTCCCGGGTTTCTGCCGTCCAGGCACCGGGGCCGGGTGAATGATGCCGTCGGAG
AGTGGAGCTGAGAGCCTGGAGCAGCCAGCTGCGCAGGTGGGGACCGGTGCAGCCTCGGCAGTGGCCACGG
CTGGGGCGGCCGGCGGCGGCCCGGACCCGGAGGCCTCCTCGGCCTCCCTGGGACGGCACCAGAGTCGGCT
GAGCTGGCCACAGGTGAAGCGGCTGGACGCGCTGCTGAAAGAGCCGATCCCCATTCACGGACGAGGCAAC
TTCCCCACGCTGAGCGTGCAGCCCCAGCAGATCGTGCAGGTAGTCCGCAGCAGCTTGGAAGAACACGGAC
TGCGTGTACACAGCGTGCGCCTGCATGGCTCAGCCGCCAGCCACGTGCTGCACCCCGAGAGCGGCCTAGG
CTACAAGGACCTGGACTTGGTGTTCCAGATGGACCTGCGGAGTGAGGCGTCCTTCCAGCTCACCAAGGCA
GTGGTCCTGGCTTGCCTGCTGGACTTCCTGCCAGCGGGAGTGAGCCGGGCCAAGATAACGCCGCTGACAC
TCAAGGAAGCCTACGTGCAGAAGCTGGTCAAAGTGTGCACCGACCTGGACCGCTGGAGCCTCATCTCTCT
GTCCAACAAGAGCGGTAAGAACGTGGAACTCAAGTTCGTGGACTCTGTGAGACGCCAGTTCGAATTCAGC
ATCGACTCCTTCCAAATCATCTTGGACTCGCTGCTCCTCTTTGGCCAGTGCTCATCCACACCTATGTCGG
AGGCCTTCCACCCTACGGTGACCGGGGAAAGCCTCTATGGGGACTTCGCTGAGGCCTTGGAGCACCTGCA
GCACCGTGTCATCGCCACACGCAGCCCCGAGGAGATCCGAGGTGGTGGCCTCCTCAAGTACTGCCACCTC
CTGGTACGGGGCTTCCGGCCAAGGCCCAGCACTGACGTTCGCGCCCTCCAGCGATACATGTGTTCCCGTT
TCTTCATCGACTTTCCGGACCTGGTGGAACAGAGGCGCATTTTGGAGCGCTACCTGGAGGCCCACTTTGG
CGGAGCGGAAGCAGCCCGCCGGTATGCTTGCCTTGTGACACTGCACCAGGTGGTCAACGAGAGCACAGTG
TGTCTCATGAGCCACGAGCGCCGCCAGACCCTGGACCTTATTGCCATGCTCGCCCTCCAGGCACTGGCTG
AACAGGGCCCTGCTGCCATGGCTGCCCTGGCCTGGCGACGTCCTGGTTCAGATGGGGTGGTCCCAGCCAC
TGTTAATTACTATGTGACCCCCATGCAGCCACTGCTGCCCCGAGCTCACTCCTATCCTACCTGGCTGCCT
TGCAACTGACTCTGATCCTGGCCAGAAGGGAATGAGCGCCATGGGGTGGGGTGGGGTCATCAGGTATGTG
TGGAGGATGTAAGCAGACACAGGCCACCTGTGCCAGCTGGCCCGGCACTATAGGGTTGGGCCTTTGAACA
GACCAGACTTTCCTGCACAAGGCTCCTATGGGCTTAAAGCCAAGTCAGGGTTCTTGACAGAAGGACCCCT
TGGTCATCACACTGCACTCTTGGACCTGCGTGACTGTGGGACACAGAGGCTGCCTGTCTGGAAATGACAA
ATTCAGCTTGTGTGGTAGCCTCAGTAGCAACACCTTGGGTTGATTTCAATGGCTTAGAAAGGGTCAGCCC
ACAGGGACCCAGATGCTCCTATAGTGGCCACAATGTGATATCATGTGACAGGGCTATTCAGTTGGGGTAC
GGTTGGGCCATTCACAGAGCCTGGGGATCCAACCTCGATGATCACTAGGAAGAGATAAACTAAGGCATAA
AGCAACTCCTTGGGGTTTGGGTGGGATGGGAGTTCAGTCTCCTGAAACCTCCTTGCCCTAAACCGTGAGG
ATTTCCCGAGGGCGGAGGTATACAGTGGTGCGACCCATACACATCCCAGTGCCTCACCCATCTCCTTTCC
CTGTGCCTTCCAGGAGCCCTGCTGTCTGGGCACTATTGTTGGAGGCTGCTGGGTTCCAAAGCTGCCACAG
TCGAGTTGACCACTCCCTCCCCCCACTTGCTTGGTAATAGGACTCTCACTTATCGGGAAGGCGGCAGATC
CCATCCCCTTGCCACACCTCTGGATTTGTTGCTATTTTGCACTCTTCCCACCAATAAAAAATGTCTGCAC
TGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4913445 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4913445 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14470 same embryo
 EMAGE:14468 same embryo
 EMAGE:14465 same embryo
 EMAGE:14469 same embryo
 EMAGE:14466 same embryo
 EurExpress:euxassay_004295 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS