EMAGE mouse gene expression spatial database

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EMAGE:14468

Dlk2 delta-like 2 homolog (Drosophila) ( MGI:2146838)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004298. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004298_01	euxassay_004298_02	euxassay_004298_03	euxassay_004298_04

euxassay_004298_05	euxassay_004298_06	euxassay_004298_07	euxassay_004298_08	euxassay_004298_09

euxassay_004298_10	euxassay_004298_11	euxassay_004298_12	euxassay_004298_13	euxassay_004298_14

euxassay_004298_15	euxassay_004298_16	euxassay_004298_17	euxassay_004298_18	euxassay_004298_19

euxassay_004298_20	euxassay_004298_21	euxassay_004298_22	euxassay_004298_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14468_wholemount_strong.wlz

14468_wholemount_moderate.wlz

14468_wholemount_weak.wlz

14468_wholemount_possible.wlz

14468_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14468_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 23 weak expression: see section 02 20 21 22

spinal cord

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 13 14 15 16

pharynx

moderate

regional

moderate expression: see section 11 12 13

esophagus

moderate

regional

moderate expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4387
Entity Detected:	Dlk2, delta-like 2 homolog (Drosophila) ( MGI:2146838)
Sequence:	sense strand is shown >T4387 TCGGCTGGCATGGCAGCTACTTCGGTGCCGAGCCCCGCGGCCAGCTAGGGGCGGCCCCCCTCCCGCCCCC CGGCCCAGTCCGTCGGATCCGGTTTGACTCTGCGCCCCGGGGACGCGTCGCCTCCCAGCCGGAGGTGTCT ACACGTCTGGCCGTTCATCCGTCCGTCCCTCCTGAGGCCGGAACTGACCATGCCCAGCGGCTGCCGCTGC CTAAATCTCGTGTGCCTGCTGTGCATCCTGGGGGCAACCAGCCAGCCTGCCAGAGCGGATGACTGCAGCT CCCACTGTGACCTGGCCCACGGCTGCTGCGCTCCTGACGGCTCCTGCAGGTGTGACCCAGGCTGGGAGGG GCTGCACTGTGAGCGCTGTGTGAGGATGCCTGGCTGCCAGCACGGTACCTGCCACCAGCCCTGGCAGTGC ATCTGCCACAGTGGCTGGGCAGGAAAGTTCTGTGACAAAGATGAGCACATCTGTACCTCACAATCACCCT GCCAGAATGGAGGCCAGTGTGTGTATGACGGGGGTGGTGAGTACCACTGTGTGTGCCTGCCAGGCTTCCA TGGACGTGGCTGTGAGCGCAAGGCTGGACCTTGTGAGCAGGCAGGCTTCCCGTGCCGGAATGGAGGGCAG TGCCAGGACAACCAGGGTTTTGCCCTCAACTTCACATGCCGCTGCTTGGCAGGATTCATGGGTGCCCACT GTGAGGTCAATGTGGACGACTGTCTGATGCGGCCTTGCGCCAATGGTGCCACATGCATTGATGGCATAAA CCGCTTTTCCTGCCTCTGTCCTGAGGGCTTCGCTGGACGCTTCTGCACCATCAACCTGGATGACTGTGCC AGCCGCCCATGCCAGAGAGGGGCCCGCTGCAGGGACCGTGTCCATGACTTTGACTGCCTGTGCCCCAGTG GCTATGGTGGTAAGACTTGTGAACTTGTCCTACCTGCTCCAGAGCCTGCCTCAGTGGGCACCCCACAAAT GCCCACCTCAGCTGTAGTTGTACCTGCCACGGGGCCGGCCCCTCACAGTGCGGGGGCGGGCCTGCTAAGG ATCTCAGTGAAGGAGGTGGTACGGAGGCAAGAGTCTGGGCTTGGTGAATCTAGCCTGGTTGCCCTGGTGG TGTTTGGGTCTCTCACTGCTGCGCTGGTCCTGGCCACGGTGCTGCTGACCCTGAGGGCATGGCGCCGAGG TATATGCCCCACTGGACCCTGCTGCTACCCAGCCCCACACTATGCCCCAGCTCGGCAGGATCAGGAGTGC CAGGTTAGCATGCTGCCAGCGGGGTTCCCTCTGTCCCCAGACCTGCCCCCTGAGCCTGGTAAGACCACAG CGCTGTGATGGGAGGAGCCTTTCTAGCTACCTTCCTGCCCTCCTTCACACCTCAGACTGTCACCAGCCCT CAGCTCTGGTACACCCATCCAGGGGATTTCTGCCTCACACCAGAAATAGTATTTTTTTAAAACACAGAAT GTAAGATAGGAATTTTATCAAATAAAATTATGAAAATGCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4913786 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4913786 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14470 same embryo
	EMAGE:14467 same embryo
	EMAGE:14465 same embryo
	EMAGE:14469 same embryo
	EMAGE:14466 same embryo
	EurExpress:euxassay_004298 same experiment
	MGI:4824325 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source