EMAGE mouse gene expression spatial database

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EMAGE:14472

Glipr2 GLI pathogenesis-related 2 ( MGI:1917770)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005658. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005658_01	euxassay_005658_02	euxassay_005658_03	euxassay_005658_04

euxassay_005658_05	euxassay_005658_06	euxassay_005658_07	euxassay_005658_08	euxassay_005658_09

euxassay_005658_10	euxassay_005658_11	euxassay_005658_12	euxassay_005658_13	euxassay_005658_14

euxassay_005658_15	euxassay_005658_16	euxassay_005658_17	euxassay_005658_18	euxassay_005658_19

euxassay_005658_20	euxassay_005658_21	euxassay_005658_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14472_wholemount_strong.wlz

14472_wholemount_moderate.wlz

14472_wholemount_weak.wlz

14472_wholemount_possible.wlz

14472_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14472_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15

arm

moderate

regional

moderate expression: see section 01 02 03 04

hand

moderate

regional

moderate expression: see section 05 06 07 08

foot

moderate

regional

moderate expression: see section 10 11 12

leg

moderate

regional

moderate expression: see section 05 06

lower leg

moderate

regional

moderate expression: see section 07 08 09

upper leg

moderate

regional

moderate expression: see section 07 09

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

tongue

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

tail paraxial mesenchyme

strong

regional

strong expression: see section 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7189
Entity Detected:	Glipr2, GLI pathogenesis-related 2 ( MGI:1917770)
Sequence:	sense strand is shown >T7189 CGCTAGCCAGCGCAGCGCAACGCAGCCGCAGCGGAGCGGAGGAGCGCACACAGCAGGCCATGGGCAAATC AGCTTCCAAACAGTTTAATAACGAGGTCCTGAAGGCCCACAACGAGTACCGGGCCCAGCACGGTGTCCCG CCCCTGAAGCTTTGTAAGAAGCTCAACCGGGAAGCCCAGCAGTATTCGGAGGCCCTGGCCAGCACGAGAA TTCTCAAGCACAGCCCAGAGTCCAGTCGTGGCCAGTGTGGAGAGAACCTTGCGTGGGCGTCTTATGATCA GACAGGAAAGGATGTGGCCGATAGGTGGTACAGCGAAATCAAGAGCTACAACTTCCAGCAGCCTGGTTTC ACTTCAGGGACTGGGCACTTTACAGCCATGGTATGGAAGAATACCAAGAAGATAGGCGTGGGGAAGGCAT CTGCGAGTGACGGGTCCTCCTTTGTGGTGGCTAGATACTTCCCAGCAGGGAATATTGTCAACCAGGGCTT CTTCGAAGAGAATGTCCCACCTCCAAAGAAGTAGCTCTCGCCAGCAAGTGTAACGAGATGGAGATGCATA TATGAAGTGCCTTTAGAACCACAAAAACGCCAGCGAGTGTCTGTCCCCGTGGGTGTGTGTATTTATGTGT TTACCTGTGAGCATCTCTCGTGACAGCTACACCTGAGTACCTTCTGATTGAAGGATGGCCATCTGAAACA TTTGTCCAGAGGGCTAGCTAACCACAATTGGGTAAGATTAGTTCTGAAATCTTTTTTCTGTTTTTGTTTG GTTGGGGTTTTGTTTGTTTGTTTTGCTTTTATTTTTGTTTTGTTTTGTTTTTTCAAGGCAGGGTTTCTCG GTGTAGCCTTGGCTGTCCTAGAATTTGCTCTGCAGACCAGGCTGGCAGAGATGCACCTGCCTGTGCCTCC CAAGTGTGGGATTAAAGGCATGAGCCACCACTGCCTGGTTTTTGTTTTTGCTTTTTAAAGCAGACTTCAT TTGAAACTTTCTTCCTTCTAATATCCATCCTTGGGCTTTGAGGATTCTGAATATTTGCCATTCTCAGAAG TGAGGTCCTTTCCTAGGCCCTTCCTGCACTGTAATCTTCTTGTGGTAGATACTGAAGGGATCGACATGTC CTTTAGGTGTGACATAAAGCAGCTAGTGTTAAAAACATTTGGCCAGCTGCATCTCACAGGACCAGTGAGA TCTCTCATTCTCAGTTGGAAGACATGATATCATCCTGCATCCTCCAGGATTCCCTAATAGAACTGCCAAA GAGCAAATGGAGATGGAAAAAAGAGCCTTACATTCTTGAAGTTTTTCAACCTTTAAAGAGCAAATACCAC AGCCACTAGCACCTCTCACACCTCATATGTGGGTTGTTTGTTTGTTCGTTTGTTTTTGTTTTTTGTTTTT AAGTTTCATTGCTCACAGCCCTACTCTGGTTCCTGGGGGTGGGGCTAGGTCTGGAATGCAGCCCCGCGTC CCCCCATCCCCCCAGTCATCTCGGGTGACCCTCACTCTGTCTGGGCTCTTGGCCAAGGCCTTGGACTATG ATGAAGTCAATAGAGCAGAGGTGAGGGTGAGCTTCCCTGGAGGAAGCCCCAAAGCCTCTGTCCTTCCCCA ACTCTGCAGGCGGTGACCACCCAGGCCTGTCCCCAGCAGCTGTGGCTACAGCTGCGCCTTGGCTTCCTCC CGGAATGTGTGACAAGCCCAGATGCTCCCGGGAGGAGGCCCAAACAAGGAGATTAGGAACGCTAATTTGA TCCTGTGTTTTGCCTCAAACTATACACAGTTTCCTCTGAAAAAACTGATTTAACCACGTCTGCCAGGGAG GGATCAAGCCAGAGGTTCAATTGTGGATCCCTGAAATGGTGGTATCTCCTCCTGCAAGGAGATAAAAGGG TTGTTGGCCTAATGACAGCAAGGCTGGCTGGTCTTTGTTACACTGTGAACAGTCCCACACTGCGCCACTT GGTTCATCCAGGGATAGCAACAAACAAACGAGACAATAAAATCCTTTCCTTTTTGTGTAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:4037002 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4037002 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14471 same embryo
	EMAGE:14473 same embryo
	EurExpress:euxassay_005658 same experiment
	MGI:4825074 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source