EMAGE mouse gene expression spatial database

EMAGE:14489

Nmb neuromedin B ( MGI:1915289)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004311. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004311_01	euxassay_004311_02	euxassay_004311_03	euxassay_004311_04

euxassay_004311_05	euxassay_004311_06	euxassay_004311_07	euxassay_004311_08	euxassay_004311_09

euxassay_004311_10	euxassay_004311_11	euxassay_004311_12	euxassay_004311_13	euxassay_004311_14

euxassay_004311_15	euxassay_004311_16	euxassay_004311_17	euxassay_004311_18	euxassay_004311_19

euxassay_004311_20	euxassay_004311_21	euxassay_004311_22	euxassay_004311_23	euxassay_004311_24

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

strong

spotted

strong expression: see section 08 16

pons mantle layer

strong

regional

strong expression: see section 10 11 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8267
Entity Detected:	Nmb, neuromedin B ( MGI:1915289)
Sequence:	sense strand is shown >T8267 AGAGCCTGTTTGGCACAGCCATGACCCGGCAAGCAGGGAGCTCTTGGCTCCTCCGTGGTCTCCTGCTCTT CGCATTGTTCGCTTCCGGCGTCGCTCCCTTCAACTGGGATCTCCCGGAGCCCCGCAGCCGAGCAAGCAAG ATTCGAGTGCACCCTCGGGGCAACCTCTGGGCGACCGGTCACTTCATGGGCAAGAAGAGCCTGGAACCCC CGAGCCTGTCACTGGTGGGGACAGCACCCCCTAACACCCCGAGGGACCAGAGACTACAGCTGAGTCATGA TCTGCTCAGGATCCTCCTGCGAAAGAAAGCTCTAGGCATGAACTTCAGTGGCCCAGCTCCCCCAATCCAG TACAGGAGGCTGCTGGAGCCACTACTGCAGAAGTGATGCCAATAATGGAACAAACCGGATGCTGGGCTTA GAATGTGTCCATCCAGGGAAGCTGACAATGGAACCCTAGCAGTGGCCTCCTCTGGATGTAAATCCTAAGC TCAAATGTGTTAATCTGTTACTGTGATTTCTGGTTGGGTCACCAGGAATGTTAATGATGCAGACACAAAG TCTTTCCTGCTGTATTTCTTGCTTCCCTGGTGAAGTGGTGAATAAAAATACTGCTCTTTAAAAAAAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1366735 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1366735 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14488 same embryo
	EMAGE:14487 same embryo
	EMAGE:14486 same embryo
	EMAGE:14490 same embryo
	EMAGE:14485 same embryo
	EurExpress:euxassay_004311 same experiment
	MGI:4826730 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source