EMAGE mouse gene expression spatial database

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EMAGE:14539

6330503K22Rik RIKEN cDNA 6330503K22 gene ( MGI:2141942)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005409. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005409_01	euxassay_005409_02	euxassay_005409_03	euxassay_005409_04

euxassay_005409_05	euxassay_005409_06	euxassay_005409_07	euxassay_005409_08	euxassay_005409_09

euxassay_005409_10	euxassay_005409_11	euxassay_005409_12	euxassay_005409_13	euxassay_005409_14

euxassay_005409_15	euxassay_005409_16	euxassay_005409_17	euxassay_005409_18	euxassay_005409_19

euxassay_005409_20	euxassay_005409_21	euxassay_005409_22	euxassay_005409_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14539_wholemount_strong.wlz

14539_wholemount_moderate.wlz

14539_wholemount_weak.wlz

14539_wholemount_possible.wlz

14539_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14539_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8770
Entity Detected:	6330503K22Rik, RIKEN cDNA 6330503K22 gene ( MGI:2141942)
Sequence:	sense strand is shown >T8770 GGGACCCGTAATATGGCGGGGAGGAGGAGGAGGAGAAGGCGGAGGACCTAGATGCGCTCTGTCTAAACCG ACTGAGGCCTTTGCTTACAGAGGAGGCTGGTCCACGGGCAGAGAAGCTGACCGGGCAGTAGGTGTCAGTG CCTACCACGTGCTGACACCATTGTGTGTCATGGCGAACCAGTGAGCAAGACTGAGAAGGTACCTGTTCTT ATGGAGTTTACGTTTTGGTGGAAGAAGATACACAAAACCCCAGGAAATAAACGAGATCATTTCAGATAGT GTGTGCGGTGGGAAGATGGAGGAATATGAGGAGTTCTGCGAGAAAGCACTTGGCCGAGCTCAGGAAGCAT CGCTGTCCACAGGCAGCTTCCTGCCCGCTCAGGCAGAAAGTGTCTCACTCATCCGTTTCCATGGGGTGGC TGTACTTTCTCCACTGCTTACCATTGAGAAGAGAAAGAAAATTCAAGAGGAAAAGCAGAAAGCACTTGAT GTGCAGTCAAGAAAGCAGGCCAACAGGAAGAAAGCCTTACTGACTCGTGTCCAGGAGATTCTTGAAAATG TTCAGGTTAGAAAAGCACCAAATGCCAGTGATTTTGATCAGTGGGCAACAGAAACCATTTACTCTAATCC AGAAGTCACAGACTTGAATGTTCCTGTTAGAGTTCCAAACAGCTTGCCGAGCCCCACCGAACACTGTACT TCAGTAAAGCTTGAAAAGATAACTGGACTTTTGCCTGTGAATAATGAGGACCAACAAACCCCTAAGCGGG TGGGGCTGCCTGGAGACTCAGAGGTGTCTGGTTCTCTGAGGCAGTGTGAGAGTCCAGAAAGCAGGCAGGC AGAAGATGGAGCTGCTCTAAGGCTTTCTTCAGCAAGCCCACAGGAGACCATCATTTCTGATGTCTTAGGA AAGGAAGAACAGGACCCATCATGTTTGGCTGAAGTCACTCCAGATCCCTACATAATGAGCCTTCAGAATC TAATGAAGAGGTCAAAGGAATACGTGGAAAGAGAACTGTCTAGCCGGAGTCTGAGAAATAGTTTAAAGAG AAGTGTTAATGAAACGCATTCAGACAGAGAAAACGATGCTGCTAAGGCGAGCGACTGTGTAAAGGAGAAG GCCCCACCCATGCCCATAGGCAGGCACTGTGGCTCTGCCATCCCTGACAAACCAAGCCTTAATAAGTCAA ATGTTCTCCTCCAAGGTGCTTCACAAGCAAGCAGCATGGGCACGGCAGGCTTAGCTAGCTTTTCTAAGAT AGACCTGCCTGCAGGAGCTGCTCCTCCCGCTGCTCCAGATGCCGGATCAGATTTTACAGTTATTCCTACC TTTGTTACTGAAAATAAAGTTAAAAGTCTTAAAGGTCCATATGCCAAATTACCTAGTCCAGAGCCAAGTA TGAGTCCTACGATGCATCGGAGGCATTCTCGGTCAGCATCAGCATGTCAGATCCTTATAAACAACCCAGT AAATGCCTGTGAGCTGAGCCCAAAGGGGAAAGAAGAGGCAGTGGATAGAACCGCTCCAGCTGCTGCTGAG ACAACAAATGAGTCTGAAACTGTGCCAAAATCACCGACTGATTTGACAGGGGTTTGTTCAAGTAATGTTT CTGCTACCAAAATCACGTCTGAGAGCACAAGAGAGATGGTCGTGGGTAAGCCAAGTCAGAGGCAGCAGGC TTTAGGAGCTCACTTAGGCAATAACGTCACTGTTGAGCGGTCTGCTATGGAAGGTCCCTTCATAGCTGAT GACAGGGGAGCCCAGAAGGTGGACGGTACTTGTATGGCTGTGCCCAAGTTGCATGAACTGCAGCCCAGCA GTCAGTGTGTGTCAAGTCAAACCTTGGAAGATGTGTGTGAACTCAAGTCAGCCAGTCTGCTAGCAAAGAA CTCCTGCAACTTACAAATGGAACTGAATAAATCTTACGATGTGAAACACCCATCTCCCTTACTTACGCAA ACCCAGACTTCCAGACAGCAGATGGACACACCGCCGGTGTTCCGTGGAAACGAGCAGTTTGTAGATAACA GTTTTGAGAAAGTTAAGCGGAGACTTGATTTAGATGTTGATAGTTTACAAAAAGAAAATTGTCCTTATAT TATAACAGCTGGAGTAGCTGAGCAGGAGCGGGACCGTCTGCTGGAAAGAAGATACCCCAAGGGATTCGTC CACATTAACAAGAATAAGATGCTAGAAACTAGCCCCAAAGAAGGCCAGGAACTACTGAAAAGCAAGATGT TAGCTTTTGAAGAAATGCGAAAGAGACTAGAGGAACAGCATGCCCAGCAGTTGTCACTCCTCATAGCTGA GCAGGAGAGGGAGCAGGAGCAACTGCAAAAGGAAATAGAAGAGCAGGAGAAGATGCTGAAGGAGAAGGCA GTTACGACGGACGTCTCTGACTTGAACAGTGCACTGGAGTGGAGACAAAGAACCGATTCTGCTTTGCTAG AAACCATGCTGTCCCAAGTGGACTCACTCCAGACTTCAAACAACTCTGGCTTCATAACTTCTGCCCTGCA GTACAGCTTCGGCTCTGCAGGTGAAGCACCGTTCTACCTCTGGGGATCACTGACCAGTGGCGTGACCAGA GTCTCAGGAACAAGGCCTTGTGGACGGGCCCAAGCTAAATGGTCTCAGGTTTTTAATCCAGAAATACACG CAAAATTTAATAAAATAACTGCCGTGGCAAAAGGGTTTCTTACTCGTAAACTTATGCAAACAGACAAACT GAAGCAGCTTCGACAAACTGTAAAAGACACCATGGAATTCATACGAAGTTTTCAGTCAGAAGCCCCTCTG AAGAGAGGTGTCGTCTCAGCCCAAGATGCTTCCCTTCAGGAACGAGTGTTAGCTCAGTTGCGAGCTGCTC TGTATGGTATTCATGACATCTTCTTCGTAATGGATGCAGCCGAGAGAATGTCAATCCTCCATCATGATCG AGAAGCCCGCAAAGAGAAACTGCTCCGGCAAATGGATAAGATGAAAAGTCCACGAGTGGCGCTTTCAGTG GCAACACAGAAGTCCCTGGACAGGAAGAAGTTCATGAAAGTTGCTGAGATGGGAATGCCAAATAAGAAGT TCCTGCTTAAACAAAACCCCTCAGAAACAAGAGTCCTTCAGCCAAACCAAGGACAGAATGCACCCGTTCA TAGGCTACTTAGTAGACAAGGAACCCCTAAGACTTCAGTGAAGGGGGTTGTGCAAAATAGACAGAAGCCT TCACAGAGCAGAGTGCCTAACAGAGCGCCTGTGTCAGGAGCATATGCAGGAAAAACCCAAAGAAAGCGGC CAAATGTTGCGACAATTTAAGAAGACAGCACTCACTGGGATGAAGCAGGGAGCAGGGCTGCCGCCACCCA CATTCCACTCTAGTTGGGGCTTGTGACTTAGCCCTGGACTCTGTTTCTGCAGGCAAGGGACAGCGAGGGC TGAGCATTGGCCTGTATAATCTGGTCAGCTTGGACAATTCCTGCCTCATTTCCTATTAATGTTAGTTTGG ATAAAAATAATTAGCATTTAGTAATTGCCTTGTTTCTGAGTTTAGCCTATATAAACATACTATATGTGCC CAGAGGTCTACAGTCACCCTTTTTTATAAACATAAAAAGACATCTAGACTTATTTTTACAAATTATTGGA AAGATATCATCAATCGTAAATTAGCCCTAAAACCTAAATTTCATTCATGAAATTTTTTTCTAATTTTAAA CACCCAGAATCCCATGTCCTTTGACTTGCAGTGTAGCTATCTAGAATGAAACACTTGCCTTGCTAATCCC AAGTAAGAAGTGACTGGGTGGGGGGTTGCGGGGAGGGGTCTTGGTTTTGGGTAGAAGCACACATTCCTGC AGACAGCTAGTTGTTTTATAGAAATGTTAGGTTTCATGTGTGTTACTATTAGGTATATATAGGCTAGATT GTGCCCACTATCCTGGAAGGTGGCAAGTTTAGAGACGATAGCACAATTTTCATGAACATAGCACTGGGGC ATGCACTGTGATGGGGCAGTGGAGCGCATGCGGCTAGTTCTAGTTAGTCTATATTGTTTGTTTTTTCAGT CTTCTTTTTCTAGCATGCTTTACATAGGAGAGAATAAAGGGCTATTTAATAAGCAATCCGGATCTCAGAG AACATAGTAATACTGTCTAGTTTAAAGCCTGCAGTAAAGGTACTCAGATTTATGGCTTTCATGACTAACA CTAAACACATCAGCCAACAATGTACCAAGAAAACATGATTTTATGAAACAGAATGTCACCATATAATTCC AAAAAGGGCTTTGTGCTTCTCATGTAACATTGGGATTGTGTAGATTTGAACCTTATAACTTGAATTTTAA AGATAGTAGCATTTTGAAGATGCTTAATAACCTTTTTAGGAATATGGAGATAAAGATTGTTTTTCTGATT TTCTTCCATCTGAAGTTTGACTTTTATACCCAGCCAGAAGTTACAAAATGATGACCCACTCTGGGTATAA GCTTGATTTCCTTTGTTGAGACTTACCCTTCATTGAAGAGAAAAAGAAGACAGTCAAAAAAGGTCCAGGT TACAAAGTTCTCCTCATAGGTGGAAAGGTTTACTGCAGTGTTATCCTCTAAGTGTGATAAACTGTGCTTT ACCGCTCCGAACACTCAGGACTCCGTGTGTATCAGTGTGGGCTCCTCAGTAAGGAAGAGGAGCTCAAAGT TTGTGTGTATTAATATGAATTAGCTAACGTGAACTATGTTTTGGAAAGTTACAACTACTTTTACATTAAG GATGAGAAACTGGAGTGTTTTTGATTTCATGGTAAAAGTTTGTGTGTTTTACATTTGAATGTTCACAGTT TTCTCTCAGAGAAGTGCCAGACATTGTTTTGTGCTTTTGAAGAAGTTTTATTTATATGCATTATTTTTAT TGTTTTTACTTTGACTGGAATGTTTATTGTGAACTGTATTTATTTTTATACTTTGATTTTCACTAGTTTT GCTTTTATGTAAAAGTCAAAATAAACTTTTCATCCTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6406475 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6406475 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14542 same embryo
	EMAGE:14538 same embryo
	EMAGE:14543 same embryo
	EMAGE:14541 same embryo
	EMAGE:14540 same embryo
	EurExpress:euxassay_005409 same experiment
	MGI:4822779 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source