EMAGE mouse gene expression spatial database

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EMAGE:14543

Myt1 myelin transcription factor 1 ( MGI:1100535)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005418. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005418_01	euxassay_005418_02	euxassay_005418_03	euxassay_005418_04

euxassay_005418_05	euxassay_005418_06	euxassay_005418_07	euxassay_005418_08	euxassay_005418_09

euxassay_005418_10	euxassay_005418_11	euxassay_005418_12	euxassay_005418_13	euxassay_005418_14

euxassay_005418_15	euxassay_005418_16	euxassay_005418_17	euxassay_005418_18	euxassay_005418_19

euxassay_005418_20	euxassay_005418_21	euxassay_005418_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14543_wholemount_strong.wlz

14543_wholemount_moderate.wlz

14543_wholemount_weak.wlz

14543_wholemount_possible.wlz

14543_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14543_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 16

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 16 17 18 19

vagus x ganglion

strong

regional

strong expression: see section 06 15

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 15 16 17

not examined

homogeneous

not examined expression: see section 08

trigeminal v nerve

strong

regional

strong expression: see section 08 15

spinal cord

strong

homogeneous

strong expression: see section 06 07 09 10 11 12 13

cervico-thoracic ganglion

strong

regional

strong expression: see section 07 08 13

cervical ganglion

strong

regional

strong expression: see section 07 14

thoracic ganglion

strong

regional

strong expression: see section 09 10 11 12

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 13 14

not examined

regional

not examined expression: see section 01 02 03 22

neural retina

strong

regional

strong expression: see section 01 02 03 weak expression: see section 22

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 14 15 16 weak expression: see section 08 09 10 11 17 18

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8775
Entity Detected:	Myt1, myelin transcription factor 1 ( MGI:1100535)
Sequence:	sense strand is shown >T8775 CTTTTTCCAGCATGAAGTGACTTGCATCCAGGCTCCAGAGAAGAGGTTCATCCATCTTGCCAGGATGACG CTCTGCGGGAGCTTCCTGGAAAATGAGTTCCGGGGCAAACTTCTGACCCAGAGGTGGACACAGGAAGACA AGACTAGTAGCTGCAGAACTGAGAGACATTTCCTGTGTAGATACAACAAGATGAGCTCAGAAAGTGATGA CAAGCGAGCCCGCACACGGTCCAAGACCCTCCGAGGACCCCCAGAGACCACAGGTGCAGACCTCAGTTGT CCTACCCCAGGATGTACTGGTTCAGGACATGTGCGGGGCAAGTACTCCAGACATCGAAGCTTACAGAGTT GCCCTCTGGCCAAGAAGAGAAAACTGGAGGATGCTGAGACTGAGCACCTGGTATCCAAGAGGAAGTCACA CCCTCTGAGGCTGGCTCTGGATGAGGGTTACCGCATGGACAGTGATGGCAGTGAGGATGCTGAAGTGAAG GACGTCTCTGTTTCAGATGAATCAGAAGGACCCCTGGAGGAGGCTGAGGCTGAGATGTCAGGACAGGAGG AGATTCATCACCCACAGACAGCTGAAGGAAAAAGCCTCATCAAGCCCCATTTTGACTCCAACCCCACAAG CAGCCCTTCTGGCTTCTCCAAGAGCAGCTACAGTAGCTACCAGGGGATCATTGCAACTTCCCTCCTAAAT CTGGGCCAAATTGCTGAAGAGGCCCTTGTGAAGGAGGATTCCGTCTCAGTAGCTAAGTTGAGCCCCACTG TTGTTCATCAGCTTCAGGATGAGGCTGCAATGGGGGTCAACAGTGACGAGGGTGAAAAGGACCTCTTTAT ACAGCCAGAAGATGTGGAGGAGGTCATTGAAGTCACAAGTGAACGTTCCCAGGAGCCATGCCCCCAGTCT CTGAAGGATATGGTTAGTGAAGAGTCAAGTAAGCAGAAAGGAGTCCTAGGTCATGAGGAGGAGGGAGAGG AGGAGGAAGAAGATGAAGAAGAAGAGGATGAGGAGGAGGAGGAGGAGGGAGAGGAAGGAGAAGAAGAAGA GGAGGAGGAGGAGGAAGAAGAGGAGGAGGAAGATGAAGAGGAAGAGGAGGAGGAGGAGGAAGCAGCTCCA AATGTTATCTTTGGGGAAGACACCTCCCATACCTCTGTCCAGAAGGCGTCTCCTGAGTTCCGAGGCCCAG AGCTATCTAGTCCTAAACCTGAGTACTCAGTCATCGTGGAGGTTCGCTCTGATGATGACAAGGATGAGGA TTCACGCTCCCAGAAGTCAGCAGTCACAGATGAATCAGAAATGTATGACATGATGACCCGTGGGAATTTA GGCCTTCTGGAACAGGCCATTGCCCTGAAGGCTGAGCAGGTGCGAGCGGTCTGTGAGTCTGGCTGTCCAC CTGCTGAGCAGGGCCATCTGGGCCCAGGAGAGCCAGGGAAAATGGCAAAGCCCCTGGACGTGGTGAGGAA GAGCTGCTACAGCAAAGATCCTTCCAGGGTGGAGAAGCGTGAAATCAAGTGTCCGACACCTGGCTGTGAT GGCACTGGCCATGTTACTGGATTGTACCCTCATCACCGCAGCCTGTCTGGCTGTCCCCACAAGGATAGAA TCCCTCCAGAGATCTTGGCCATGCATGAAAATGTACTGAAGTGCCCTACTCCTGGCTGCACGGGCCAAGG CCATGTGAACAGCAACCGCAATACTCACAGAAGTTTATCTGGATGTCCCATTGCTGCTGCTGAAAAATTA GCCAAATCTCATGAGAAGCAGCAATTGCAGACAGGAGATCCTCCCAAAAATAACTCCAATTCAGATCGGA TCCTCAGGCCCATGTGTTTTGTGAAGCAGCTTGAGGTTCCACCTTATGGGAGCTACAGACCCAATGTGGC TCCCGCCACGCCCAGGGCCAACTTGGCCAAGGAGCTGGAGAAGTTCTCCAAAGTCACCTTTGACTACGCA AGTTTTGATGCTCAGGTTTTTGGCAAACGTATGCTTGCCCCAAAGATTCAGACCAGCGAAACCTCACCCA AAGCCTTCCAATGCTTCGATTACTCTCATGATGCTGAGGCTGCACACATGGCTGCCACAGCCATCCTGAA CCTCTCCACTCGATGTTGGGAGATGCCAGAGAACCTCAGCACAAAGCCACAGGACCTCCCCAGCAAGGCT GTGGACATTGAGGTAGATGAAAATGGAACTCTGGACTTGAGCATGCATAAACATCGCAAGCGGGAAAACA CTTTCCCCAGCAGTAGCAGCTGCAGTAGCAGCCCTGGTGTCAAGTCTCCTGATGTCTCTCAACGTCAGAG CAGTACCAGTGCCCCCAGCAGCTCTATGACCTCACCCCAGTCCAGCCAGGCCTCTCGCCAGGATGAGTGG GACCGTCCTCTAGACTACACCAAACCTAGTCGGCTTCGAGAGGAAGAGCCTGAGGAGTCAGAGCCAGCAG CACACTCTTTTGCTTCTTCTGAAGCAGATGATCAGGAGGTGTCAGAAGAGAACTTTGAGGAGCGGAAGTA TCCAGGGGAAGTCACCCTAACCAACTTTAAGCTGAAGTTTCTTTCCAAGGACATAAAGAAGGAACTTCTC ACCTGTCCCACCCCCGGCTGTGACGGCAGTGGCCATATCACCGGGAACTATGCCTCCCATCGCAGCCTCT CTGGTTGCCCTCTTGCTGACAAGAGCCTCAGAAACCTCATGGCTGCCCACTCTGCTGACCTCAAGTGCCC CACACCTGGCTGTGATGGCTCTGGCCACATAACAGGGAACTATGCTTCACACCGGAGTTTGTCAGGCTGC CCACGTGCCAAGAAGAGTGGACTCAAGGTGGCGCCTACCAAGGATGACAAGGAGGACCCCGAGTTGATGA AGTGCCCAGTTCCAGGCTGTGTGGGGCTCGGCCACATCAGCGGCAAATATGCCTCTCACAGGAGTGCTTC TGGCTGCCCACTGGCTGCTCGGAGGCAGAAAGAGGGAGCTCTCAATGGTTCATCTTTCTCGTGGAAGTCG CTGAAGAATGAGGGCCCTACTTGCCCCACCCCAGGTTGCGACGGCTCTGGCCATGCCAACGGCAGTTTCC TCACCCACCGAAGTTTGTCTGGCTGTCCCAGAGCAACCTTTGCTGGAAAGAAAGGAAAACTCTCAGGGGA TGAGATTCTCAGCCCAAAGTTCAAGACAAGTGATGTGCTGGAGAATGATGAGGAGATCAAGCAGCTAAAT CAGGAGATACGAGACTTAAATGAGTCCAATTCGGAAATGGAGGCTGCCATGGTGCAGCTGCAGTCTCAGA TCTCGTCCATGGAGAAGAACCTGAAGAACATCGAGGAGGAGAACAAGCTCATTGAGGAGCAGAATGAGGC CCTGTTTCTGGAATTGTCCGGGCTTAGCCAAGCCCTCATCCAAAGTCTTGCCAATATTCGCCTTCCACAC ATGGAACCAATATGTGAACAGAATTTTGACGCCTATGTGAACACCCTCACTGACATGTACTCCAATCAGG ACTGCTACCAGAATCCGGAGAACAAAGGCCTTCTGGAAACGATCAAGCAAGCTGTGAGGGGCATTCAGGT CTAGGCTGCACTGCACACAGGAGTCTGCTCTGCTCCTCACAGCAAGGACCCTGACTTCTAGCTACTTCTA ATTGGAAGCCTGGGTGCAGCCTTGTGCAGCTTACCCAAAGGGGAGCCTGGGAATCAGTGTCTCCTATGAT GTCCTCATTAGGCCCAGGCATGAAGACCTCTCTGTGTGAGTGCCTGGGGACACTGAAGTATTGCAAAGTG ACTTATTTTTGTCTTTGTTCTTTGTTTTTTGTTTTTTTAAAAGAAGTCTGAAGAGCAGCTCAAAGTCTCC GGTGGAAGCTCATGGACAAGGTTCTCACTCAGGGAAGTTTTGGAGTTTGCAGCCACAGTATTCCATTGTT TGTCAAGGTTGGGAGGGCAGCCATGGCCCTGCTGGGTGTTAGTCTCAGCAGGAGGGCATGCCTAGGTTTC TGACTATGCTCACTGAAGCCCACATGACAAGTATGTTTTGTGTTTTCATTTAATTCAACTTTGTGTTAAA GGTGCACAATACTCTGCTTCAGAGGTGAATCTAGGAACAGGCTTCCCAGATGGCAGTCTACACAGTTGAT GAGCATCATAGAAAGTCAGCTGGAGGTTTTTTTTTGTTTTCAGTTGGAGGCCCTGAAAAATCGGCCACTT ACTTGGGCAGCAGATGCCTTTAAGCTGTGCAGATCTGCTTTCTCCTGGCTCCAGTTTTTAACCCCTGGCT TGGGCTCTAAGTCCAAGACCACTGGGCCAAACCTCTGGTTTCCGTACCTCCATCCAGGTGCTGGACCCAT CATTGTATCTTTTCCTGCATAGGTTTGCTCCTTGTATCCAAGGTGACTGCAGTGGGGCAGGAGACCAGTG GTAAATTGTTACAGGGAGACCACCAAGATTTCACTTCTTTATAGGTTCATTTAAGATGTGAAAAGGATAA ATTTTAATTTAATGTGTTAAAAACACAATGTTCTTACTGTGTAAATAGAGTCCAAGTCAATTGTGACTGC AATTCCAAATTAGTATTTAAAAGTAGAGAAATTGCACTTCCTGGAAGACACTGAAAGTAGTTAGTTTATT CTGTAAATTATTTAATTTTGTCATAAGTATTTATATTCTTACCCTCTTATGTTAATGTTTGCTATTTATT ATTTTTATTTATTTATTTTATACTATATCAACTAGATGACACACTAGGGCACCATAAGAGTTAAATAAGT TAAATTGAAACAAAAACATTTATTTGACTATAAGATAGGCCACAAAGAATCTTGATATAACTTCTAGTTA TAATTTTGATGCAACCGAGTTATTTTTTATATATTTTGTTATTTATTCTTTTAATAATGGAATTTTTTAA AAAAGTATTTTGTAACTGAAGAATTTTGATAATTTGGGTTTTTCTCCCCATTGGTCCACCAGAGAGAAAG GTGTTTGGTTTTCTTTTTCCCTTTGGGTTCCTTTTGTCAGGCCAATAACAGCAAATGGAATTCTGTATTT ATTATTATTTAAGTGTAGTGCAGATGTGTGTGCTCTGGTGTTTCTTGTGTTGCATTTGTGGAGGTTCTCA TGGATGATTCATGTTGCTGTGAGGGAGTGCTGGCCATGGTCAGTTCTAGGCAATGGACTCGCTGGCCAAG CTGCCCAGGATCTTGATACCCCAATACTGTGACCCTCCTTCCTCAGGAAACACGTTGGACTCACAGTACC TCGTGGTGTGTTTGGAGGGTGATTTCTAATAAAAGTCTCACTGTGCAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:6406740 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6406740 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14542 same embryo
	EMAGE:14539 same embryo
	EMAGE:14538 same embryo
	EMAGE:14541 same embryo
	EMAGE:14540 same embryo
	EurExpress:euxassay_005418 same experiment
	MGI:4826576 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source