EMAGE mouse gene expression spatial database

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EMAGE:14568

Robo2 roundabout homolog 2 (Drosophila) ( MGI:1890110)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005435. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005435_01	euxassay_005435_02	euxassay_005435_03	euxassay_005435_04

euxassay_005435_05	euxassay_005435_06	euxassay_005435_07	euxassay_005435_08	euxassay_005435_09

euxassay_005435_10	euxassay_005435_11	euxassay_005435_12	euxassay_005435_13	euxassay_005435_14

euxassay_005435_15	euxassay_005435_16	euxassay_005435_17	euxassay_005435_18	euxassay_005435_19

euxassay_005435_20	euxassay_005435_21	euxassay_005435_22	euxassay_005435_23	euxassay_005435_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14568_wholemount_strong.wlz

14568_wholemount_moderate.wlz

14568_wholemount_weak.wlz

14568_wholemount_possible.wlz

14568_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14568_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8808
Entity Detected:	Robo2, roundabout homolog 2 (Drosophila) ( MGI:1890110)
Sequence:	sense strand is shown >T8808 GAGACAGGGCTAGACGCACGTGAATAGTCCCGCCAGCTGGGCTGAATTTGTGGGAATTTAGAAAGACAGC CTGTGGAAGTGCAACGTCTCTGAAGTCCCCTGGGTTCATTCGGATGGCACCTAACGCGTCCCGTGACAGA CCTCTTCACCAACAGCTTCCGATGTTGCCATTTTGCTCTTCTTGACCTTAATTAATCTCTAGGAAAGTCT AAACTTCGGACCTACCTCTTTTTTTGATACTTATTTTTGTACTTCTGCTCTCTGGGATTGGTTTCTTAAA CAACCTGGATCCTTTTTCATATGTCAAAATGAATCCTCTGATGTTTACACTATTATTGCTCTTTGGATTT CTCTGCATTCAGATTGATGGATCTCGTCTTCGTCAAGAAGACTTTCCCCCCAGGATCGTAGAACACCCTT CTGATGTCATCGTCTCCAAGGGAGAGCCCACCACTCTGAACTGTAAAGCAGAGGGCCGACCCACCCCCAC CATTGAATGGTACAAGGATGGTGAGAGGGTGGAGACAGACAAGGATGATCCCAGGTCCCACAGAATGCTT CTGCCCAGCGGATCTTTATTCTTTTTGCGAATTGTTCATGGGCGCAGAAGTAAACCGGACGAAGGGAGTT ACGTTTGTGTTGCAAGGAACTATCTTGGTGAAGCAGTGAGTCGAAATGCATCTCTGGAAGTGGCATTATT GCGAGATGACTTCCGGCAAAACCCCACAGATGTGGTAGTCGCAGCTGGAGAGCCTGCAATCTTGGAGTGC CAGCCACCACGGGGACACCCAGAACCAACCATCTACTGGAAAAAGGACAAAGTCCGAATTGATGACAAGG AAGAGAGAATAAGTATCCGTGGTGGGAAGCTGATGATCTCTAATACTAGGAAAAGCGATGCTGGCATGTA CACCTGTGTGGGAACCAATATGGTGGGAGAAAGGGACAGCGACCCTGCAGAGCTCACTGTCTTTGAACGA CCCACATTTCTCAGGAGGCCAATTAACCAGGTGGTGCTAGAGGAAGAAGCTGTAGAATTCCGTTGTCAGG TCCAAGGAGATCCCCAGCCAACGGTGAGGTGGAAAAAAGATGATGCAGACTTGCCGAGAGGAAGGTATGA TATCAAAGATGACTACACGCTGAGAATTAAAAAGGCCATGAGTACTGATGAAGGTACCTATGTGTGTATT GCTGAGAATCGGGTGGGAAAAGTGGAAGCCTCTGCTACCCTCACTGTCCGAGTTCGCCCTGTTGCTCCTC CACAGTTTGTGGTTAGGCCAAGAGATCAGATCGTTGCTCAAGGCCGAACAGTGACATTCCCCTGTGAAAC TAAAGGAAACCCACAGCCAGCTGTTTTTTGGCAGAAAGAAGGCAGCCAGAACCTACTTTTCCCGAATCAA CCTCAGCAGCCCAACAGCCGATGTTCAGTGTCGCCCACGGGGGACCTCACCATCACCAACATCCAGCGTT CAGATGCGGGTTACTACATCTGCCAGGCCCTAACCGTGGCAGGAAGCATTTTAGCTAAAGCACAGTTGGA AGTTACTGACGTTTTGACAGATAGACCTCCACCCATAATCTTGCAAGGACCAATAAACCAAACACTTGCA GTAGACGGTACAGCATTGTTGAAGTGTAAAGCCACTGGTGAGCCTCTGCCTGTAATTAGCTGGCTAAAGG AGGGCTTTACTTTTCTGGGGAGAGATCCAAGAGCCACGATCCAAGACCAAGGAACACTGCAGATTAAGAA TTTACGGATATCTGATACTGGCACTTATACTTGTGTGGCTACAAGTTCCAGTGGAGAGACTTCCTGGAGT GCAGTGCTGGATGTAACAGAATCTGGAGCAACAATCAGTAAAAATTATGATATGAATGACCTCCCGGGAC CACCATCCAAACCTCAGGTCACTGATGTTTCTAAGAACAGTGTCACCTTATCCTGGCAGCCAGGTACACC TGGCGTTCTTCCTGCAAGCGCGTATATCATTGAGGCTTTCAGCCAATCGGTGAGCAATAGCTGGCAGACA GTGGCAAACCATGTTAAGACAACTCTGTATACAGTAAGGGGGCTGAGGCCCAACACAATCTACTTGTTTA TGGTCAGAGCGATCAACCCACAAGGTCTCAGTGATCCAAGTCCTATGTCGGATCCTGTACGCACACAAGA TATCAGCCCCCCAGCACAAGGAGTGGACCACAGACAGGTGCAGAAGGAATTAGGTGATGTCGTTGTTCGT CTCCATAATCCAGTTGTCCTGACACCTACAACTGTTCAAGTCACATGGACGGTGGACCGACAACCCCAGT TTATTCAGGGCTACAGAGTGATGTACCGTCAGACTTCGGGACTACAAGCCTCAACTGTGTGGCAGAATCT AGACGCCAAAGTCCCGACTGAGAGGAGTGCTGTCCTTGTGAATTTGAAAAAGGGGGTGACTTATGAAATT AAAGTCCGGCCGTATTTTAACGAGTTCCAAGGAATGGACAGTGAATCGAAAACAGTCCGAACCACTGAGG AAGCCCCAAGTGCCCCTCCCCAGTCTGTCACTGTGCTGACAGTTGGAAGTCACAACAGCACAAGCATCAG TGTTTCCTGGGATCCTCCACCAGCCGACCACCAGAATGGAATTATTCAGGAATATAAGATCTGGTGTCTG GGAAACGAAACGCGATTCCATATCAATAAAACGGTGGATGCAGCCATTCGCTCTGTAGTAATAGGTGCAT CCACCAGCCGACCACCAGAATGGAATTATTCAGGAATATAAGATCTGGTGTCTGGGAAACGAAACGCGAT TCCATATCAATAAAACGGTGGATGCAGCCATTCGCTCTGTAGTAATAGGTGGCTTGTTCCCTGGAATTCA GTACCGGGTAGAAGTGGCAGCTAGCACAAGTGCAGGGGTTGGAGTAAAAAGTGAACCACAGCCGATAATA ATTGGGGGACGTAACGAAGTTGTCATTACTGAAAACAATAACAGCATCACTGAGCAAATCACGGATGTCG TGAAGCAACCGGCATTTATAGCTGGCATTGGTGGTGCCTGCTGGGTAATTCTGATGGGTTTTAGCATCTG GTTGTACTGGAGAAGAAAGAAGAGAAAGGGACTCAGCAATTATGCTGTAACATTTCAAAGAGGAGATGGA GGACTAATGAGCAATGGGAGCCGTCCAGGTCTTCTAAATGCTGGCGATCCCAATTACCCATGGCTTGCTG ATTCTTGGCCAGCCACGAGTTTGCCAGTGAACAATAGCAATAGTGGCCCAAATGAAATTGGAAATTTTGG GCGTGGAGATGTGCTGCCTCCGGTGCCAGGCCAAGGGGATAAAACAGCGACCATGCTCTCGGATGGAGCC ATTTATAGCAGCATTGACTTCACTACCAAAACCACTTACAACAGTTCCAGCCAAATAACACAGGCCACCC CATATGCCACTACACAAATCCTGCATTCAAACAGCATCCACGAACTGGCAGTTGATCTTCCTGATCCACA GTGGAAAAGCTCAGTTCAACAGAAGACAGACCTCATGGGATTTGGTTATTCGCTACCTGATCAGAACAAG GGGAACAACGGTGGGAAAGGTGGAAAAAAGAAGAAAACTAAAAATTCTTCGAAAGCGCAGAAAAACAACG GATCCACTTGGGCTAATGTCCCTCTACCTCCTCCTCCCGTCCAGCCCCTTCCTGGTACAGAGCTGGGCCA CTATGCTGCGGAACAAGAAAATGGCTATGACAGTGACAGCTGGTGCCCACCATTACCGGTGCAAACATAC TTGCATCAGGGTATGGAAGATGAGCTGGAAGAAGACGAAGATCGGGTCCCAACACCTCCTGTTCGCGGCG TGGCCTCTTCACCAGCTATCTCTTTTGGACAGCAGTCCACTGCCACTCTTACTCCATCCCCACGGGAAGA GATGCAACCCATGCTGCAAGCTCACTTGGATGAGTTGACAAGGGCCTATCAGTTTGATATAGCAAAACAA ACATGGTAAATATCCTCCATAAACCAATGGCCCTGTACTTTCTACATATGGAGATTTTCTTGCATGTATA TTAAGTAGAGATGCTCATAAATCTATGTGAGTGGAATGAAAATTGATTTTGAATATGTGTTAACCATCCA TATTAAGAAACTGGTGACAGAAACATACGTAAACTGGCCATCATCGCACAGCTAGCTAGAGAAGTGAATG GAGCAGGGACTCAGGCTGAGTGTTTTGGTGACACCAGATTCTTTTCTCATTCACAGACCAGCGTTCATTA GTTTATCCTTGAACTCATTTTTAATGCTGCAATAAAGAACTCCTTTTAAATTCAAAAAAAAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6409283 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6409283 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14569 same embryo
	EMAGE:14571 same embryo
	EMAGE:14570 same embryo
	EurExpress:euxassay_005435 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source