EMAGE mouse gene expression spatial database

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EMAGE:14585

Flcn folliculin ( MGI:2442184)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005508. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005508_01	euxassay_005508_02	euxassay_005508_03	euxassay_005508_04

euxassay_005508_05	euxassay_005508_06	euxassay_005508_07	euxassay_005508_08	euxassay_005508_09

euxassay_005508_10	euxassay_005508_11	euxassay_005508_12	euxassay_005508_13	euxassay_005508_14

euxassay_005508_15	euxassay_005508_16	euxassay_005508_17	euxassay_005508_18	euxassay_005508_19

euxassay_005508_20	euxassay_005508_21	euxassay_005508_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14585_wholemount_strong.wlz

14585_wholemount_moderate.wlz

14585_wholemount_weak.wlz

14585_wholemount_possible.wlz

14585_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14585_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4888
Entity Detected:	Flcn, folliculin ( MGI:2442184)
Sequence:	sense strand is shown >T4888 GGCGACAGCCCCAGGACAGTTGCGAGCGGGTTCCGGCCCAGCATCCGGGAGACGGCGGCAAGCGCCCCAG CTGGGTTGGTGTTGGGCCATAGGGCTGAATGGAAAGCGCGGATGACAACCTCAAGTCTTTGAATTCGAAT AGTGCAGCTTGCTTACCTGACTCTTCCGGCGGGCCTCGTACATGTTCTGCTCTAGGCGGGATGGTGCAGT TGTGATGTGCTAAGCATAAGGCCTCGGCCATTCTCCAGCACCATGAACGCCATAGTCGCCCTCTGCCACT TCTGCGAGCTCCATGGCCCCCGCACGCTCTTCTGCACGGAAGTTCTACACGCTCCCCTGCCCCAGGGGGC CGGAAGTGGGGACAGTCCTGACCAGGTTGAGCAGGCTGAGGAGGAGGAGGGTGGCATTCAGATGAGCAGC CGGGTCCGTGCCCACAGCCCAGCCGAGGGTGCCAGCAGTGAGTCCAGCAGCCCGGGGCCCAAGAAGTCGG ACATGTGTGAGGGCTGCCGGTCACTTGCCGTAGGGCACCCAGGCTATATCAGTCATGATAAAGAGACCTC TATTAAGTACGTCAGTCACCAGCACCCCAACCACCCGCAGCTCTTCAGCATCGTCCGCCAGGCCTGTGTC CGGAGCCTGAGCTGTGAGGTATGCCCTGGTCGTGAAGGCCCCATCTTCTTTGGTGATGAGCAGCACGGCT TTGTGTTCAGCCACACCTTCTTCATCAAAGACAGCCTGGCCAGAGGCTTCCAGCGCTGGTACAGCATCAT CGCCATCATGATGGATCGGATCTACCTCATCAACTCCTGGCCCTTCCTGCTGGGGAGGATCCGCGGCATC ATCAGTGAGCTCCAGGCCAAGGCCTTCAAGGTGTTTGAGGCAGAGCAGTTTGGATGTCCACAGCGTGCCC AGAGGATGAACACTGCCTTCACGCCCTTCCTGCACCAGAGGAACGGCAACGCTGCCCGCTCTCTGACCTC CTTGACCAGTGATGACAACTTGTGGGCGTGTCTGCACACTTCCTTTGCCTGGCTCCTGAAGGCATGCGGT AGCAGGCTGACAGAAAAGCTCTTAGAGGGCGCTCCCACAGAGGACACCCTGGTCCAGATGGAGAAGCTTG CTGACTTGGAGGAAGAATCAGAAAGTTGGGACAATTCTGAGGCTGAGGAGGAGGAGAAAGCTCCTGTTAC ACCAGAGGGTGCTGAAGGGCGAGAGCTGACCAGTTGCCCAACAGAGTCATCCTTTCTCTCAGCCTGTGGG AGCTGGCAGCCCCCAAAGCTTACCGGCTTCAAGTCTCTTCGACACATGAGACAGGTCTTGGGTGCTCCAT CCTTCCGTATGTTGGCTTGGCATGTCCTCATGGGGAATCAGGTGATCTGGAAAAGCAGAGATGTGAACCT GGTCCATTCAGCGTTTGAAGTCCTCCGGACCATGCTGCCTGTGGGCTGTGTCCGCATCATCCCTTACAGC AGCCAGTATGAGGAGGCCTATCGCTGCAACTTCCTGGGGCTCAGCCCTCCCGTGCCTATCCCTGCCCATG TTCTGGCCTCAGAGTTCGTAGTTGTCGTGGAGGTCCACACGGCCACTCGCTCAAACCTCCACCCTGCTGG GTGCGAGGATGACCAGTCCCTCAGCAAGTATGAGTTTGTGGTGACCAGTGGTAGCCCTGTGGCTGCAGAC AGAGTTGGGCCCACTATCCTGAATAAGATTGAAGCAGCTCTGACCAACCAGAACCTGTCTGTGGATGTGG TGGACCAATGTCTCATCTGCCTCAAGGAGGAATGGATGAACAAAGTGAAAGTCCTGTTTAAATTCACCAA GGTAGACAGTCGCCCCAAGGAGGACACACAGAAGCTCCTAAGCGTCCTAGGCGCATCAGAGGAGGACAAC GTCAAACTGCTGAAGTTCTGGATGACGGGACTGAGCAAAACCTACAAGTCCCATCTCATGTCCACCGTCC GAAGCCCCACAGCTACAGAGTCACGGAGCTGACTCCGAGAACTCCTTCTGGAAGGTGGTGTACAGACCAG CTCTGTGGGAAAAACTGCCCTTGGGTTTCTGACTTCTGGGGTGAGGCCCTGTTTATGGCCTAGGGTTCAC CCTCCTTGTAAGACTCTATCAGCCCATGTTGAAATGTAGGGGACACAGAGACAGTGGTCCCTTTGCATCA AACTGCGCTGTCAGGACCTGGCGAGATGTGTTCTGTGCCCCGTGAGGACAAAGGCTGGGAACTGAGAGGA GTCCATCAGTCTGCCTCTGAGGTTTTGTTGTTGGAGCGTGTCCTCATGGGGTTAGGACAACGCCTTCCCA CCATGGAAGACAGCAAGCATCGTGGATCATTTGTCTTTCGGTTGGTGACTTCTCAGGTCACGTGCACAGC CGTGGCCTCTGCACATCTTGCCGGCCTGTGGATACATCAAAACCATGGCTGTTTTGTTTGCTTTTGTTTT TTTTTAATTTTGGTTTTTGAGTCAGGGTCTTATTCTGTATCCTATCCCAGTTTAGAACTCATGGTGTGGC CTCTGCTACTTCCAGACTTGTAGCAATCCTCCTGCCTCAGCCTCCCAAGTGCTGGGATTACCGAACTGAG CCATCAGAACATTACATTGCTTTCTTCTCTGTTGCTGCCGTAGCGCTAACCAGCCACTGTAGACTATGAC GGTGTTACGACAGATCGCCTCTCACCATTGCAGTTTAATAAGGACGTAGCTTGGAAAGATTTCCTGAAAG CTTTCCCAATTTGTTTTTATAATATTCTTGGTGTTTGTTTATATTTAACATGAAGACTGTGTATCTCAGT CTCTGTACAGTTGCAAGAATGTTGAAACACAATAAATCTGTATTTTTGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5099493 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5099493 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14584 same embryo
	EMAGE:14587 same embryo
	EMAGE:14586 same embryo
	EMAGE:14588 same embryo
	EurExpress:euxassay_005508 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source