EMAGE mouse gene expression spatial database

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EMAGE:14588

Aspscr1 alveolar soft part sarcoma chromosome region, candidate 1 (human) ( MGI:1916188)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005504. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005504_01	euxassay_005504_02	euxassay_005504_03	euxassay_005504_04

euxassay_005504_05	euxassay_005504_06	euxassay_005504_07	euxassay_005504_08	euxassay_005504_09

euxassay_005504_10	euxassay_005504_11	euxassay_005504_12	euxassay_005504_13	euxassay_005504_14

euxassay_005504_15	euxassay_005504_16	euxassay_005504_17	euxassay_005504_18	euxassay_005504_19

euxassay_005504_20	euxassay_005504_21	euxassay_005504_22	euxassay_005504_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14588_wholemount_strong.wlz

14588_wholemount_moderate.wlz

14588_wholemount_weak.wlz

14588_wholemount_possible.wlz

14588_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14588_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4902
Entity Detected:	Aspscr1, alveolar soft part sarcoma chromosome region, candidate 1 (human) ( MGI:1916188)
Sequence:	sense strand is shown >T4902 CGGACGCGTGGGGGCGAGCCGGCTGCGGTCACGTGAGCGGAAGATGGCGGCCCCGGCGGGCGGCGGGGGC TCCGCGGTGTCGGTGCTGGCCCCAAACGGGCGGCGCCACACGGTGAAGGTGACGCCGAGCACCGTGCTGC TGCAGGTACTAGAAGACACATGCCGGCGGCAAGACTTCAACCCCAGTGAATACGACCTGAAGTTTCAGAG GACGGTGCTAGACCTCTCTCTCCAGTGGAGATTTGCCAACCTGCCTAACAATGCCAAGCTGGAGATGGTG CCTGTGTCCCGTAGCCGCGAGGGGCCTGAGAACATAGTTCGCATCGCGTTCCAGCTGGATGACGGCTCCA GGTTGCAGGACGCTTTCTGTTCCCGACAGACTCTCTGGGAGCTTCTCAGCCATTTTGCACAGACCAGGGA GCGTCTGCAGCAGCTGGGTGAGAAGACCCCTGTCTGCGTGTACATGAGGAATGAGGTGACTGGTAGAGCT GCCTTACAGAACACAACCCTTCAGTCACTGGGCCTGACAGGTGGTAGTGCCACCATCAGGTTTGTCATAA AGCAGTGTGACACCGCCGGCAAACAGGAGTCCATAGCCGTCAGGAGCAAGGCCCCGGGAAGTCCGGTCTC CTCCTTGTCAGCCGACCAGGCATCCAGTAGCACATTGCTTCCTCTGAACTCTGGGGAGTTCAGCAGGGGA GACCTAAACCATGAGGGTGATGCAAACACCTCAGGGACCGGCCTTGAGGGTGGCCCCAAACCAACGGATG CTCAAACAAAGCAAAGCACCAGTGAGCCTGCATCGGCCCCCTTCGTTCCCTTCTCTGGGGGGGGCCAGCG GCTGGGGGGCCCGTCTGCGTCCTTGAGACCTCTAACATCACCTTCGGCCAATTCATCCAAGTCTTTCTCT GGCCCTGGGGGCCCTTCTAAGCCCAAGAAGCCAAAGCCTGGTGAGGAGCCCCAGCAGGAGCCTGAACCGC CCGTTGACCGAGATCCTGTGGTGTACCACCCTGACCTAGAGGACTTGCTACAGCCCTGGCCGGCAGAGGT GCCTGACGAGTTCTTTGAGGTGACTGTGGATGATGTGAGGAGACGCTTGGCCCAGCTCAAGAGTGAGCGG AAGCGTCTGGAAGAAGCCCCCCTGGTGACCAAGGCTTTCAGGGAGGCTCAGATGAAGGAGAAACTGGAAC GTTACCCAAAGGTGGCTCTGCGGGTCCTATTCCCTGACCGCTACATCCTGCAGGGCTTCTTCCGCCCCAG TGAGACAGTGGGGGATCTTCGAGACTTTGTTAGGAGCCACCTGGGGAACCCCGAGCTCTCCTTTTACCTA TTCATCGCTCCACCCAAAATGGTCCTGGATGACCACACGCTGACTCTCTTTCAGGCAAACCTCTTCCCTG CTGCGCTTGTGCACTTTGGAGCTGAAGAACCAACAGGTCTCTATCTGGAACCTGGGCTGCTAGAACACAC CGTCTCCCCATCTACAGCTGATGTGCTGGTGGCCAGGTGTATGTCCCGGGCTTCCGGGTCCCCACCTCTG CTGCCAGCCCCTGACCCTGTATCCCTGGAGTCTGAGCCAATCGCTGAGGATGGGGCACTGGGGCCCCCAG AGCCCATCCAAGGGACAGCCCAGCCTGTGAAGAGAAGCCTGGGCAAAGTACCCAAGTGGCTGAAGCTGCC AGCCAGCAAGAGGTGAGAGCTACCAGCCTGGAGATGCCTATCACCAGCCACAGGACCCTACCCCCACCCA CCCCGAGCAGGAATAAAGAGTCGCGCTTCTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5101238 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5101238 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14584 same embryo
	EMAGE:14587 same embryo
	EMAGE:14586 same embryo
	EMAGE:14585 same embryo
	EurExpress:euxassay_005504 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source