EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14645

AA415398 expressed sequence AA415398 ( MGI:3035485)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005486. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005486_01	euxassay_005486_02	euxassay_005486_03	euxassay_005486_04

euxassay_005486_05	euxassay_005486_06	euxassay_005486_07	euxassay_005486_08	euxassay_005486_09

euxassay_005486_10	euxassay_005486_11	euxassay_005486_12	euxassay_005486_13	euxassay_005486_14

euxassay_005486_15	euxassay_005486_16	euxassay_005486_17	euxassay_005486_18	euxassay_005486_19

euxassay_005486_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14645_wholemount_strong.wlz

14645_wholemount_moderate.wlz

14645_wholemount_weak.wlz

14645_wholemount_possible.wlz

14645_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14645_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9612
Entity Detected:	AA415398, expressed sequence AA415398 ( MGI:3035485)
Sequence:	sense strand is shown >T9612 CAAGCATCTCCTGGCAATGGGAATTCTAAAGGTTTATCCACTCATTTGTCCAACAAAGACATAAAAAGTC ATTGTACCAGGCACTACTGTAGTGCTTCAGATTAATCAGTGAACAGAAAAGGCCGGTGGAGGGGCAAGTA TCATTCCCTCATGGGCAGAAAGGCATTGTTTCTACTGATATGAAAATTGCAACCTCCTGGGGCTAGCCAC TGCAAGAAGACGTCTTAGCGGCTGTCCTGATGACCATTGCTGCTCAGGGTAAAGGCTGTTCCATCTGCAG CAGACCCAGAATGGAAAACATGTGCAGCAAAGCCACAGCAGACCTGGGAAAGACCCAACAAATGAGGACA ACCACCCATTGGACCACTGAGAGAGAGCTGAGAGTCACGGCAGCAACGTAACAGAGCTTATACAGACTGT TTCTGAACCCTTTTCTGGAACGTCTTAAAGTGCTGGCATCCTTTCCAGGAACATTTGAGTTTAGCCTTGA AAAATGAATGGGAGCTTGAAGCAAGACACATGGGAGAGTGGTTCCAGGAATTAAAGGCCCAGAGTTGGAA GGCCTGCTTCTATTTTGAATCTCTAAACTGGGAACACACACCTTTAATCCAGATCTTTGGGAAGACCCAC CTATAATCCAGGCCATGCCTTCTGCTAGAGGCCTTATAAAGAACATGAAAGATGGAAGCCTCTGTCTTTG CCGGCTTGTTCTCCCCTTGCTAGCCAGTATCTTCCCCCACTGGCCTTGCATCAGACTTGATTGGGATTCC AGCATATAGTGAAGACCACCTTAGACATCCAGCCTTGTGAAATAAACAACTACTGGACTCTTGGATATTT TGTTCACAAGCAACCACTGTTGGATTAGCCAGACAACAAGCTGGAAGAAGAGAAAGCCTTTCAGTTCAAC CTGAGCATGGATTCAGAAACACCCCATCCAGGCCCTTCCTGTCAACTGCTGGACCTCCCCACTGGACCAC AGACCACCTCTGTCAGGACATTAGGATCAGAACCAAGTAAAAATGAGCAGAGGTCCAGCCAATCAGACAG CAATTCCAGAAAGCACACTGAAGAATCAGCTCAGATGGTGGAAACACCCAGGAAAAGAAGACGCTCTTCC AAGGACTCCAGCCTGGTCAGAATAGGAAATGAGGGCAGCTTGCATGGGAAAAAGAGACACAAGACTTCAG TAAAGAAGACTGAGCCAAAAGCCAATGGTCACCAGGATGGATTAGCCCATTGCCCAGAACCTCAGGATAC AGACCCACCACCCATTCGGAAGAGCCTGGTGAGCTTTCTGCGGGGGAAGTCTGAGGAGATTTATAGAGAC ACAGTCCAGATGCAGGCCCAGAAGCTTGGCTCCCTGCTCACCCCTGAGCAGCTCTCTCAGCTCAGACAGC TCTCAGAGTCTCTGACTGCCATGGTATACAGCCTTCTACAGCATGGCCAACCAGGAGGCTTTGCCTTCCC TGCTGAGGGCTGGCTTGTCCCAGCCCCCATGGCTGCCCCTCAGGAGCTGTCTGGCAAGGAATCTCAGAGT CCTTTGATGGAGGGAGGAGAGAAGATAACAGATCCTGCCATCCCATCAGACAAGTCCTGAGCCAAGGCCA TGAGACCATCCTAAGCAGAGTCACATCTCGAGACACCAAAGAACCGTTCCATCTCCCTGTCTACACTGCC TGTGGCACTCACCCTCTCTGTGTGAACATGTAACAGGCACATCGATGAGAAGAAGAGTTTGAAATGCTTA TCTGGATTATGAACTGTGTTTCTCTGGGACCTAAGATTCAAGTGCTAAATGCACAGTCGATCCGTGCTTC CTTTGGATATTTTGCTAAGTGTGTGTTAGATTTGTATACTTATATTGTAGCGTGGCAAATAAATACTGAT TTACCAGAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30625085 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30625085 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14643 same embryo
	EMAGE:14642 same embryo
	EMAGE:14647 same embryo
	EMAGE:14646 same embryo
	EMAGE:14644 same embryo
	EurExpress:euxassay_005486 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source