EMAGE mouse gene expression spatial database

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EMAGE:14676

Uox urate oxidase ( MGI:98907)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005615. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005615_01	euxassay_005615_02	euxassay_005615_03	euxassay_005615_04

euxassay_005615_05	euxassay_005615_06	euxassay_005615_07	euxassay_005615_08	euxassay_005615_09

euxassay_005615_10	euxassay_005615_11	euxassay_005615_12	euxassay_005615_13	euxassay_005615_14

euxassay_005615_15	euxassay_005615_16	euxassay_005615_17	euxassay_005615_18	euxassay_005615_19

euxassay_005615_20	euxassay_005615_21	euxassay_005615_22	euxassay_005615_23	euxassay_005615_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14676_wholemount_strong.wlz

14676_wholemount_moderate.wlz

14676_wholemount_weak.wlz

14676_wholemount_possible.wlz

14676_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14676_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4966
Entity Detected:	Uox, urate oxidase ( MGI:98907)
Sequence:	sense strand is shown >T4966 CTTGAAATCGAACCTGAAGTTAAAGGCTGAGGGAAAAATGGCCCATTACCATGACAACTACGGAAAGAAT GATGAAGTGGAATTTGTCCGAACTGGCTATGGGAAAGACATGGTCAAAGTTCTCCATATTCAGAGAGATG GAAAATACCACAGCATCAAAGAGGTGGCAACTTCGGTCCAGTTGACTCTGAGGTCCAAGAAGGATTACCT ACACGGTGATAATTCCGACATCATCCCTACAGACACCATCAAGAACACTGTGCACGTCCTGGCGAAGCTC AGAGGGATCAGAAACATCGAGACCTTTGCAATGAACATCTGTGAGCACTTCCTCTCTTCTTTTAACCATG TCACTCGAGCCCACGTCTACGTGGAAGAGGTCCCCTGGAAACGATTTGAAAAGAATGGGATCAAACACGT CCATGCATTCATCCACACCCCGACGGGAACGCACTTCTGTGAGGTGGAGCAGATGAGAAACGGACCTCCC GTCATTCACTCTGGAATCAAAGACCTCAAGGTCTTGAAAACAACCCAGTCTGGGTTTGAAGGGTTCCTCA AGGACCAGTTCACTACCCTCCCTGAGGTGAAGGACCGATGCTTTGCCACTCAAGTGTACTGCAAGTGGCG CTACCAGAGACGGGACGTGGACTTCGAGGCTATCTGGGGCGCTGTCCGGGACATTGTCCTGCAGAAATTT GCTGGGCCCTATGACAAAGGTGAATACTCACCTTCCGTGCAGAAGACCCTCTATGATATACAAGTGCTGT CCCTGAGCCAGCTTCCTGAGATAGAAGACATGGAAATCAGCCTTCCGAACATTCACTACTTTAACATCGA CATGTCCAAAATGGGACTGATCAACAAGGAAGAGGTTTTGCTGCCTCTCGACAATCCCTACGGCAAAATA ACAGGGACAGTCAAGAGGAAGCTGCCTTCCAGGCTGTGACGTCATGGCTCCAGCCGGTTGCGATGCTGGG GAATACTGTATGCTTGCCTGCAAAAATTTCAACTCAGGATATTGATTGCTTTGTTCTGTATGAAGTAATT GCTTTGTTGTTATTGTTGTTCCTAGTTCAACAGGGAACTTGTAGTGGTATGTATGTGGAGTCCCATGTTA GCAGGCCCAACCTGCTCCACGGCCATGGGCAGTTTCCTTAAAAATTACTGGGCCATGTTAGGAGAATCAC AGTGATAGTTCTCCTTGCTGTTCTTGTGTTCTCTGCCTTTGAATCCGTCTTTAGAATCCAATTGTCATAA GGACATAATCGTAAAGTACTGGCTGCCGCCACATTGATTAATCTGAACACAGAAGCAAACACTGTCATCA TCCTAGGGATATGTCTGGGTCTAACTGAAAACAGAATAAAACCATGTGAAAAAAAAAGTCACCATAATGT TTTTTAAAAACTCTGGCACTCTGACTAGCACTCACATGGAGGAAGGCGGGTGGGCATGAAACTCCAATCC TAGCTCAGGAGTTATTGGTGTTTGATTGAGGCTGGAAGAGGGAAAGTCAGCTTCATTTAGGGGTGTGACA CCTGGTATGTCAATCATACTGAAGGACACCAGGTTGCATGCCTAGAAGTGGTCCGATAAACCCAAACTGG CCTCAGTGGGAAAGAGAAAGAACATGAAGTTGGGTGAGTGAGAAAGACTTGGGATAAACTGGGATGGGAA GTGAATATTATGAAACTTATTGTATAAAATTCTCAAATAATTAATAAAAACATTATTTTAAATGTTTAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5136328 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5136328 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14679 same embryo
	EMAGE:14677 same embryo
	EMAGE:14680 same embryo
	EMAGE:14675 same embryo
	EMAGE:14678 same embryo
	EurExpress:euxassay_005615 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source