EMAGE mouse gene expression spatial database

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EMAGE:14679

Rbck1 RanBP-type and C3HC4-type zinc finger containing 1 ( MGI:1344372)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005604. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005604_01	euxassay_005604_02	euxassay_005604_03	euxassay_005604_04

euxassay_005604_05	euxassay_005604_06	euxassay_005604_07	euxassay_005604_08	euxassay_005604_09

euxassay_005604_10	euxassay_005604_11	euxassay_005604_12	euxassay_005604_13	euxassay_005604_14

euxassay_005604_15	euxassay_005604_16	euxassay_005604_17	euxassay_005604_18	euxassay_005604_19

euxassay_005604_20	euxassay_005604_21	euxassay_005604_22	euxassay_005604_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14679_wholemount_strong.wlz

14679_wholemount_moderate.wlz

14679_wholemount_weak.wlz

14679_wholemount_possible.wlz

14679_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14679_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2724
Entity Detected:	Rbck1, RanBP-type and C3HC4-type zinc finger containing 1 ( MGI:1344372)
Sequence:	sense strand is shown >T2724 TGGCCTCGAGGCCAGATTCGGCACGAGGCAGAGGAGATGGCCCTGAGCCTTGCCCGGGCAGTGGCTGGCG GAGATGAACAGGCTGCTATCAAGTATGCCACCTGGCTGGCAGAGCAGAGGGTGCCCCTCAGGGTGCAAGT AAAACCCGAGGTCTCCCCAACACAGGACATCAGGCTCTGTGTGAGTGTGGAGGATGCGTACATGCACACC GTCACCATTTGGCTCACAGTACGCCCGGATATGACAGTGGCCTCCCTTAAGGACATGGTATTCCTGGACT ATGGCTTCCCACCTAGCCTGCAGCAGTGGGTGGTTGGACAGAGGCTGGCACGAGACCAGGAGACCTTGCA TTCACACGGCATTCGGCGGAATGGAGACGGTGCCTATCTCTACCTGCTGTCAGCCCGCAACACGTCACTC AACCCACAAGAGCTGCAGCGGCAGCGGCAACTGCGAATGTTGGAAGATTTGGGCTTCAAGGACCTCACCC TTCAGTCACGGGGGCCCTTGGAACCTGTCCTTCCGAAGCCCAGGACCAACCAGGAGCCGGGACAGCCAGA TGCAGCACC
Notes:	The probe template was PCR amplified from IMAGE:1513065 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1513065 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14677 same embryo
	EMAGE:14680 same embryo
	EMAGE:14675 same embryo
	EMAGE:14678 same embryo
	EMAGE:14676 same embryo
	EurExpress:euxassay_005604 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source