EMAGE mouse gene expression spatial database

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EMAGE:14683

Acat1 acetyl-Coenzyme A acetyltransferase 1 ( MGI:87870)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005559. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005559_01	euxassay_005559_02	euxassay_005559_03	euxassay_005559_04

euxassay_005559_05	euxassay_005559_06	euxassay_005559_07	euxassay_005559_08	euxassay_005559_09

euxassay_005559_10	euxassay_005559_11	euxassay_005559_12	euxassay_005559_13	euxassay_005559_14

euxassay_005559_15	euxassay_005559_16	euxassay_005559_17	euxassay_005559_18	euxassay_005559_19

euxassay_005559_20	euxassay_005559_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14683_wholemount_strong.wlz

14683_wholemount_moderate.wlz

14683_wholemount_weak.wlz

14683_wholemount_possible.wlz

14683_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14683_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5396
Entity Detected:	Acat1, acetyl-Coenzyme A acetyltransferase 1 ( MGI:87870)
Sequence:	sense strand is shown >T5396 GGAGGCCGCGGCGCGTCTCTCTGCCCTTGACAGCCTTTCGCGTCTCCATGGCTGCCCTGGTGGCTCTGCA CGGCGTCGTCCGCAGGCCTCTGCTTCGCGGGCTGCTGCAGGAAGTAAGATGCCTGGAACGAAGTTATGCA TCCAAACCCACTTTGAATGAAGTGGTTATAGTAAGTGCTATAAGAACTCCCATTGGATCCTTCCTGGGCA GCCTTGCCTCTCAGCCGGCCACTAAACTTGGTACTGCTGCAATTCAGGGAGCCATTGAGAAGGCAGGGAT TCCAAAAGAAGAAGTGAAGGAAGTCTACATGGGCAATGTCATCCAAGGGGGTGAAGGACAGGCCCCTACC AGGCAAGCAACACTGGGCGCAGGTTTACCTATTTCCACTCCATGCACCACAGTAAACAAGGTTTGTGCTT CAGGAATGAAAGCCATCATGATGGCCTCTCAAAGTCTTATGTGTGGACATCAGGATGTGATGGTGGCAGG CGGGATGGAGAGCATGTCCAATGTCCCATACGTAATGAGCAGAGGAGCAACACCATATGGTGGGGTAAAA CTTGAAGACCTGATTGTAAAAGACGGGCTAACTGATGTCTACAATAAAATTCATATGGGTAACTGTGCTG AGAATACTGCAAAGAAGATGAATATCTCACGGCAGGAACAGGATACGTACGCTCTCAGCTCTTACACCAG AAGTAAAGAAGCGTGGGACGCAGGGAAGTTTGCCAGTGAGATTACTCCCATCACCATCTCAGTGAAAGGT AAACCAGATGTGGTGGTGAAAGAAGATGAAGAATACAAGCGTGTTGACTTTAGTAAAGTGCCAAAGCTCA AGACCGTGTTCCAGAAAGAAAATGGCACAATAACAGCTGCCAATGCCAGCACACTGAACGATGGAGCAGC TGCTCTGGTTCTCATGACTGCAGAGGCAGCCCAGAGGCTCAATGTTAAGCCATTGGCACGAATTGCAGCA TTTGCTGATGCTGCCGTAGACCCCATTGATTTTCCACTTGCGCCTGCATATGCCGTACCTAAGGTTCTTA AATATGCAGGACTGAAAAAAGAAGACATTGCCATGTGGGAAGTAAATGAAGCATTCAGTGTGGTTGTGCT AGCCAACATTAAAATGCTGGAGATTGACCCCCAAAAAGTAAATATCCACGGAGGAGCTGTTTCTCTGGGC CATCCAATTGGGATGTCTGGAGCCCGGATTGTTGTTCATATGGCTCATGCCCTGAAGCCAGGAGAGTTCG GTCTGGCTAGTATTTGCAACGGAGGAGGAGGTGCTTCCGCCCTGCTGATTGAGAAGCTGTAGACAACCTG TTTTAGGAGACAGTTCCATGTGACCGGCTGAAGTAAATGTGACTCCCTTGGGCCAGGTTATATTCAGCAT AAGCTATTTCATTTTTTATTATTTTCTACTAAAATTTTTAAAAAATCATATCCAAAAAACACACTTAAAT TACAAATAAAAATTTCTTCTTTATTTTATTTTATTTTTTTGTAAACTTAAACAGTTGGATACTCTATTAT TTGAAATACCAATATAGGTATTTGAGGCATGGCTCAGCAGTTAAGAGAATTTGTTGCTAGTTGGGTGGTA CATGGCTTTAATCCCAGCACTTGGAGATAGAGGCAAGTGGATCTTTCTGAGTTTAAGGCTAGCCTGGTCC ACAGAGCTACTTCTATGACAGCCAAGAATACACATAGAAGTAGAAACTCTGGAAACAAACAAACGAACAA AAGAGCATCTGTTGCTCACACAGAAAACATTTGGTTCCCAGGACCCACACTGGGTGACACAGCAGGGAGG ACTTGGACACTGCACTGCACGCAGAAACCAGAGAAATGAGGTCCTGTGCTTTTCTACCTCACTGCTGCCG GTACAGCAGAAGCCAGCCTTGGGGATAACCTGTAACCGGGTTCTCTCCTGATGAAGGCAGGGTAAAGTTC CAGCATTTTTACATCTAAATACACCAAAGAAATGAGAATATCACAATAGCAGCAACAAAGTTCCTTGACT CTCCCAAGCCCCAAAATAATCTAACATCCATGGACTTCTATTCTGCCATTAGAATGAACACTGGCTGTTT ATGTTTTGTTTTGTTTTCGAACTTTAATCCTTGGACAGTCAAAATTGGTTTCAAATAAAGTTATGTGAAT GAAAATTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5365469 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5365469 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14681 same embryo
	EMAGE:14686 same embryo
	EMAGE:14685 same embryo
	EMAGE:14682 same embryo
	EMAGE:14684 same embryo
	EurExpress:euxassay_005559 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source