EMAGE mouse gene expression spatial database

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EMAGE:14685

Egflam EGF-like, fibronectin type III and laminin G domains ( MGI:2146149)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005579. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005579_01	euxassay_005579_02	euxassay_005579_03	euxassay_005579_04

euxassay_005579_05	euxassay_005579_06	euxassay_005579_07	euxassay_005579_08	euxassay_005579_09

euxassay_005579_10	euxassay_005579_11	euxassay_005579_12	euxassay_005579_13	euxassay_005579_14

euxassay_005579_15	euxassay_005579_16	euxassay_005579_17	euxassay_005579_18	euxassay_005579_19

euxassay_005579_20	euxassay_005579_21	euxassay_005579_22	euxassay_005579_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14685_wholemount_strong.wlz

14685_wholemount_moderate.wlz

14685_wholemount_weak.wlz

14685_wholemount_possible.wlz

14685_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14685_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5544
Entity Detected:	Egflam, EGF-like, fibronectin type III and laminin G domains ( MGI:2146149)
Sequence:	sense strand is shown >T5544 GGGACTTGGGGAAACTTTGCAGACGCCGTTGGTGAGATGGATTTAATCTCAACCTTCTCGCTCCATTTCT TGCTCCTGGCCTGCAGCCTCCCACCCGGCGCTGTGTCACTCCGAACGGCTCTGCGAAAATCAGGCAAGGT GGGACCTCCTCTTGACATCAAGCTGGGAGCCTTGAACTGTACAGCTTTCAGCATCCAGTGGAAAACACCA AAGCGTTCTGGTAGCTCCATCATTGGCTACACAGTGTTTTACTCTGAGGTTGGTTCAGATAAGTCCCTGC GGGAGCGGTCACACAACGTCCCGGTTGGCCAGGATACCCTAATCACTGAGGAAGTGATTGGAGATTTGAA GCCAGGCACTGAATATCAAGTAAGCGTAGCAGCCTACAGTCAGACTGGCAAGGGGAGGCTTAGCTTCCCT CGACATGTGACCACTTTGTCTCAAGATTCCTGCCTGCCCCCTGCAGCACCCCAACAGCCACATGTCCTTG TGGTTTCCGATTCTGAAGTGGCCCTTTCTTGGAGACCTGGAGAGAATGAAGGAAGTGCCCCAATTCAGTC CTACTCTGTGGAATTCATTAGACCAGATTTTGACAAGAGCTGGACCATAATCCAAGAGCGCCTGCAGATG GACTCCATGGTTATCAAAGGCCTTGATCCAGATACCAACTACCAGTTTGCTGTGAAAGCCATGAATGCTC ACGGCTTCAGTCCGCGCAGCTGGCCCAGCAACACCGTCCGAACCCTGGGTCCCGGGGAGGCTGGAAGTGG TCATTATGGACCTGGGTATATCACCAATCCCGGTGTGAGTGAAGATGATGATGGATCTGAAGACGAACTG GACCTGGATGTATCCTTTGAGGAGGTTAAACCACTTCCTGCTACCAAAGTTGGGAATAAGAAGTTCTCAG TGGAGTCCAAGAAGACTTCTGTCTCCAACTCAGTGATGGGCTCCAGACTCGCCCAACCTACCTCAGCCTC TCTCCACGAAACTACAGTGGCCATCCCACCCACACCAGCCCAACGGAAGGGGAAGAACAGTGTGGCCATG ATGTCAAGGCTCTTTGACATGTCCTGTGATGAAACTCTCTGTTCAGCTGACAGCTTCTGTGTCAATGACT ACGCCTGGGGGGGCTCACGATGCCATTGCAACCTGGGCAAAGGAGGCGAGGCCTGCTCAGAAGATATCTT TATTCAGTATCCTCAGTTCTTTGGCCACTCCTACGTAACCTTTGAGCCTCTGAAGAACTCTTATCAGGCA TTTCAGGTTACTCTGGAGTTCAGGGCAGAGGCGGAGGATGGCTTGCTGCTCTACTGCGGAGAGAGTGAGC ACGGAAGAGGAGACTTCATGTCCCTGGCTCTCATCCGGCGGTCCCTCCACTTCAGGTTTAATTGTGGAAC TGGGATTGCCATCATCATAAGTGAGACCAAAATCAAACTTGGGGCCTGGCATACGGTGACACTGTACAGA GATGGCCTGAATGGGATGCTGCAGTTGAATAATGGCACCCCAGTGACAGGCCAGTCCCAGGGTCAGTACA GTAAAATTACCTTCCGGACACCTCTGTACCTCGGGGGCGCTCCCAGTGCTTACTGGCTGGTCAGAGCAAC AGGGACAAACCGTGGCTTCCAAGGCTGTGTGCAGTCACTTTCTGTTAATGGGAAGAAGATTGACATGAGG CCCTGGCCATTAGGAAAAGCCCTCAATGGAGCTGATGTTGGGGAGTGCAGCAGTGGGATCTGTGACGAAG CCTCCTGCATCCACGGGGGCACCTGCGCAGCCATCAAGGCCGACTCCTACATCTGCCTCTGTCCCCTTGG GTTCAGAGGTCGACACTGTGAAGATGCTTTTGCCCTGACCATCCCTCAGTTCAGAGAGTCGCTGAGGTCC TATGCTGCCACACCCTGGCCCCTGGAGCCTCAGCATTATCTTTCTTTCACCGAGTTCGAGATCACCTTTC GGCCAGACTCGGGGGATGGTGTCCTTTTGTACAGCTACGACACGGGCAGCAAGGACTTCCTCTCCATCAA TATGGCTGCGGGCCACGTGGAATTCCGCTTCGACTGTGGTTCTGGGACGGGAGTTCTCAGGAGTGAGGCT CCCCTCACCCTAGGCCAATGGCATGACCTACGTGTGTCTCGCACAGCGAAGAATGGGATCTTACAGGTGG ACAAGCAGAAGGTGGTAGAGGGAATGGCAGAGGGAGGCTTCACCCAGATTAAGTGTAATACGGACATTTT TATTGGTGGAGTCCCAAATTACGATGATGTCAAGAAGAACTCGGGGATCCTCCATCCATTTAGTGGGAGC ATTCAGAAGATCATCCTCAACGACCGGACCATCCATGTGAAGCATGACTTTACATCGGGCGTGAATGTGG AGAACGCAGCCCACCCCTGTGTAGGGGCCCCCTGTGCCCATGGGGGCAGCTGTCGGCCCAGGAAGGAGGG TTATGAGTGCGACTGTCCCTTGGGCTTCGAAGGCCTGAACTGCCAGAAAGAGTGTGGGAACCACTGCCTC AATACTATTATAGAAGCCATCGAGATCCCACAGTTCATCGGCCGCAGCTACCTGACGTATGACAACCCAA ATATCCTCAAGAGGGTGTCTGGATCGAGGTCAAATGCGTTCATGAGGTTTAAGACAACCGCCAAGGATGG CCTGTTGCTGTGGAGGGGGGACAGCCCCATGAGACCTAACAGTGACTTCATTTCCCTGGGTCTTCGGGAT GGAGCCCTGATATTCAGCTACAACCTGGGCAGTGGCGTGGCTTCCATCATGGTGAACGGTTCCTTCAGCG ATGGCCGGTGGCACCGAGTCAAGGCTGTCAGGGATGGCCAGTCCGGGAAGATTACAGTGGATGACTACGG AGCCAGAACAGGCAAGTCACCGGGCTTGATGCGGCAGCTTAACATCAACGGTGCTCTGTACGTGGGTGGA ATGAAGGAAATCGCTCTGCACACGAACAGACAATACCTGCGAGGCCTTGTGGGCTGCATCTCCCATTTCA CACTGTCCACCGATTACCACATTTCCCTCGTGGAAGACGCCGTGGATGGGAAGAACATCAACACTTGTGG GGCTAAGTAACACCAGCTGACCTTGCCCAAGGGACATAGCCTCTATCCTGCAATCACAGAGGCCCGGAGA CCCTGCCTGATGCTGCACTCAGAGGTCTTGAAATCAGGTGTGTTTACTTTCTCTGAGGAGGACAAAGTAC ACCCTGGTGAGAGCCAAGACTGAAGGCCCTGGGTGGCCTTCTCTGCTAACGCCCTGTGAACCGAACCCAA TGGACTACTGTGGTTAGGAAGCATTGGACTTTGTTGTTCAACGTATAGAGTCTGAGAGAGATGATACGTC AGTGCAAGCTCTGGGAACTCTCTGCTGTAGCTCAATGGCTCTGTTGTGTTAACAAGAGTGAGAGCCAGAC TGGATGAGAAAGAGTGAAAGAGCAAGAGAGAGAGAGAGACAAGACAGAGAGACAGAGAGACAGAGAGACC ACCCCTCCAAATAGTATTAAAATATTTCTGCTCTTCCCTGACTTATGACACTAGCAGGAAGACTGGGCAA CCCTGAACTTCATACTTCCAATCTCGGTCTATGGATTGTGTAGATTAACCCCTTCCATGCCCTGCTGGCT GACTCAATGTGCTCTGACTAGTCCATGGAAATAAATGTTGGGGGTAAATTAAACATAGAATAATAATCAG ACTGTGGCATCCTTCCCCCAAGCTTTCCTCTAATGCAAATATTCTCTCTTTTTTTAATGATGCAGATCGA AGAAAGCGGGCCCCCTGATTTTGTAGCTGTACTTTTTGTATGTGACTATTTTTTATAGCTGCAGTCTGTC CACACCCATGGTGTGCTTTTTGAAAGTTAGTGGTTGATCCTAATTACGAAACACCTTCCTAATAGCTTTC TCACAATGGAGATGTGATCAGGCCATTTGTAGTGTCAGGGGAGCGGTAGCAAGTGACTAGGGTGGGCCAT TGGGAGAAGGGACCATTCTCTTTTAGTATATGCTGACGGTTCAAGTGGATGAATATGATGCTGTCCAAAA GTGGAGAGATTAAAAGGAATGTATCACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6467602 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6467602 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14683 same embryo
	EMAGE:14681 same embryo
	EMAGE:14686 same embryo
	EMAGE:14682 same embryo
	EMAGE:14684 same embryo
	EurExpress:euxassay_005579 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source