EMAGE mouse gene expression spatial database

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EMAGE:14686

Racgap1 Rac GTPase-activating protein 1 ( MGI:1349423)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005572. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005572_01	euxassay_005572_02	euxassay_005572_03	euxassay_005572_04

euxassay_005572_05	euxassay_005572_06	euxassay_005572_07	euxassay_005572_08	euxassay_005572_09

euxassay_005572_10	euxassay_005572_11	euxassay_005572_12	euxassay_005572_13	euxassay_005572_14

euxassay_005572_15	euxassay_005572_16	euxassay_005572_17	euxassay_005572_18	euxassay_005572_19

euxassay_005572_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14686_wholemount_strong.wlz

14686_wholemount_moderate.wlz

14686_wholemount_weak.wlz

14686_wholemount_possible.wlz

14686_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14686_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 10 11 12

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 08 09 10 12 13 14

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 moderate expression: see section 01 02 03 20

midbrain ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15

dorsal grey horn

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6693
Entity Detected:	Racgap1, Rac GTPase-activating protein 1 ( MGI:1349423)
Sequence:	sense strand is shown >T6693 GTCGCTGGTAGACAGCAGCCCTCCTGGGGCGCGGCGGCCGGAGAGTGAGCGCCGCGGGCCGGACGGGGTT GCGTGTGCGCGGGGCCGGGAGCCTCGAAAGATGGATACTACAATGGTGAATTTGTGGACTCTGTTTGAGC AGCTTGTGCGCCGGATGGAGATTATCAATGAAGGAAATGAAAGCATTGAATTCATCCAGGTTGTGAAGGA CTTCGAGGACTTCCGAAAGAAGTATCAAAGAACCAACCAGGAGCTGGAGAAATTCAAAGACCTATTGTTG AAAGCAGAGACGGGGCGGAGCGCCCTGGACGTGAAGCTGAAGCATGCCCGTAATCAAGTGGACGTGGAGA TCAAGCGGAGGCAGCGCGCTGAGGCAGAGTGTGCAAAGCTGGAACAACAGATTCAGCTGATTCGAGACAT ACTCATGTGTGACACATCTGGCAGTATTCAGCTGAGCGAGGAACAAAAATCAGCTCTCGCTTTCCTCAAC CGAGGCCAAGCATCCAGTGGCCACGCCGGCAACAATAGACTGTCAACGATTGATGAATCTGGTTCCATTT TATCAGATATCAGCTTTGACAAGACTGATGAATCACTGGACTGGGATTCTTCTTTGGTGAAGAATTTCAA AATGAAGAAACGAGAGAAGAGGCGCTCCAACAGTAGACAGTTCATCGACGGCCCTCCTGGGCCTGTGAAG AAAACTTGTTCCATTGGCTCTACAGTAGACCAGGCAAATGAATCAATAGTTGCAAAAACTACAGTGACTG TTCCCAGTGATGGGGGACCCATTGAAGCTGTGTCTACTATTGAGACATTGCCGTCCTGGACCAGGAGTCG CGGGAAGTCAGGACCTTTACAACCTGTGAACAGTGACTCCGCTCTGAACAGCAGGCCACTGGAGCCAAGA ACTGACACAGACAATTTGGGCACACCTCAGAATACTGGAGGCATGCGCTTGCACGACTTCGTCTCAAAGA CGGTTATTAAGCCTGAATCTTGTGTTCCGTGTGGAAAGCGGATCAAGTTTGGCAAGCTGTCTCTGAAGTG TCGAGACTGTCGTTTGGTCTCCCATCCAGAATGTCGGGACCGATGTCCCCTTCCCTGCATCCCCCCCCTG GTGGGGACACCGGTTAAGATTGGAGAGGGCATGCTGGCCGACTTCGTGTCGCAGGCTTCTCCCATGATCC CTGCCATTGTCGTCAGCTGTGTCAATGAGATCGAGCAGCGAGGCCTGACTGAGGCAGGCTTGTACAGGAT CTCAGGCTGTGACCGCACAGTGAAAGAACTGAAAGAAAAATTCCTTAAGGTGAAAACTGTGCCCCTCCTC AGCAAAGTGGACGATATCCACGTCATCTGCAGCCTCCTGAAGGACTTCCTGCGCAACCTCAAAGAGCCCC TCCTGACCTTCTGGCTGAGCAAAGCCTTCATGGAGGCAGCAGAGATAACAGATGAAGACAACAGCACAGC CGCCATGTACCAGGCTGTCAGTGAGCTGCCCCAGGCCAACAGGGACACGCTAGCCTTCCTTATGATCCAC CTACAGAGAGTGTCTCAGAGTCCAGACACTAAGATGGATATTGCCAATCTAGCTAAAGTCTTTGGCCCTA CAATAGTTGCCCATACTGTGCCCAATCCAGATCCAGTGACAATGTTCCAGGACATCAAACGTCAGCTCAA GGTGGTGGAGCGCCTACTCTCTCTCCCCTTGGAGTACTGGAATCAGTTCATGATGGTGGACCAAGAGAAC ATAGACAGCCAGCGAGGCAATGGAAACTCAACACCACGCACACCAGACGTTAAAGTGAGCTTACTGGGGC CTGTGACCACTCCTGAATTCCAGCTTGTCAAGACTCCTTTATCAAGTTCCCTGTCACAGAGGTTGTACAA CCTCTCCAAGAGCACACCCAGATTTGGGAACAAGAGCAAGTCTGCCACCAACTTAGGTCAACAGGGCAAA TTTTTCCCTGCTCCGTACCTCAAGTAAAGCTGTGTCTGCCTGTGTTTACTGCACGAGACACCCCTGTCTG CTCTTCAGCCTCCTGTGTAATGACTACTTTTAGCATTTTCCAGACTTTAAATAAAGTTGAACGCGTATGA GAGTTTGAGTGTTGCATAGCTCCCTCCCACCTGGACACTTAGCACCTTTACTAGTTGTCGGGAGCTTTAA AATAGGAGATCTTTACCAGGGCCGAAGGGGAAATGGGTATAAGTTGGGGCTCTTAGGGGGAGGGGAGAGG AGATCCCTTTGGCTTAAAGCCAAATACTGCTCATGAAATGACTTTGCTGTGGTGTCACTTAGACAATGAC AGAAACTGTACCTCTTAGGTAACGTCTTAGTTATCTCAGGACAGGATAAAGAGCTGCGCAGCGGCCCCTT CTCAGAACACAGGCCTCTTCCTCCTTCGGGGACTGACAAGAAGCTTGGAGCCCTCAGCGGGATGGGCCGG GCCACTGATCTGAGGATGCTTCACTTCTGTCTGGGTTAAGCTGAGAAAGTGCCACAGGCTCTGATCTCAC ACAGCGTGCTGTTTCTAGCCAGCCTCTGCCTGGATTTTGCAGTCAAAGTCAGGTTGATCTGAAGCCGAAT TCGTTCTGATGCCTGACCCCCTTGTTTGTCCAGTGGAGCCCATTTAAAAAGTCAGCTAGCAGTTCAGAAG ACAATTTTCATATAGCAAGAAAGTTAGCTGCTTCTGTCTTTGGTGAAGTTTTTCTGTGAAACAGATCTAT ATATGTGAACATTGGCTTTTTTGGTTAGCATTTTCCAGTGTTCAAAATGGCTTCCTTCCCTGGGATGTTT TCTGACCCATACTAACCCTTACCTGTAACATGTATCTGGAATATTATGTGGAAAAAATAAATAGCTTTTT CAAAGTGAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3602242 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3602242 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14683 same embryo
	EMAGE:14681 same embryo
	EMAGE:14685 same embryo
	EMAGE:14682 same embryo
	EMAGE:14684 same embryo
	EurExpress:euxassay_005572 same experiment
	MGI:4827588 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source