EMAGE mouse gene expression spatial database

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EMAGE:14692

Npr2 natriuretic peptide receptor 2 ( MGI:97372)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005611. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005611_01	euxassay_005611_02	euxassay_005611_03	euxassay_005611_04

euxassay_005611_05	euxassay_005611_06	euxassay_005611_07	euxassay_005611_08	euxassay_005611_09

euxassay_005611_10	euxassay_005611_11	euxassay_005611_12	euxassay_005611_13	euxassay_005611_14

euxassay_005611_15	euxassay_005611_16	euxassay_005611_17	euxassay_005611_18	euxassay_005611_19

euxassay_005611_20	euxassay_005611_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14692_wholemount_strong.wlz

14692_wholemount_moderate.wlz

14692_wholemount_weak.wlz

14692_wholemount_possible.wlz

14692_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14692_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand

strong

regional

strong expression: see section 01 02 03 20 21

foot

strong

regional

strong expression: see section 01 02 03 19 20

fibula

strong

regional

strong expression: see section 01 21

tibia

strong

regional

strong expression: see section 01 21

femur

strong

regional

strong expression: see section 01 02 03 20 21

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 13 14 15 16

axial skeleton

strong

regional

strong expression: see section 10 11

cranium

strong

regional

strong expression: see section 04 05 06 17 18

basioccipital bone

strong

regional

strong expression: see section 04 05 06 16 17

basisphenoid bone

strong

regional

strong expression: see section 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4975
Entity Detected:	Npr2, natriuretic peptide receptor 2 ( MGI:97372)
Sequence:	sense strand is shown >T4975 CTTCTGCCACCCTATCCTTAGTCCCTGGACCTGGCTGTGGGCTGGTAGCCCACTCCTTCCCGACCCAGGT TTCCCCCCATCCTCTTCTTGCGTGGTCCCCAGCACTTTCTGTATCCGGGCCGACTAAGCTTGCCCCCACT TCTCTTCCTGGCCCTCTTCCCCAGGCTCCAGGCTGGGTGGCGCTTGTGCCTGCCCAGTAGGCCAGAGCGG CCCCTGGTTCGGGGGACCGTGGGTCAGCTGCTCTATCCCCATGGCACTGCCATCCCTGCTGCTGGTGGTG GCAGCCCTGGCAGGTGGGGTGCGTCCTCCGGGGGCACGGAACCTGACGCTGGCGGTGGTGCTGCCAGAAC ACAACCTGAGCTATGCCTGGGCCTGGCCACGGGTGGGTCCTGCTGTGGCACTGGCTGTGGAGGCACTGGG CCGGGCACTGCCCGTGGACCTGCGGTTTGTCAGCTCCGAACTAGACGGCGCCTGCTCTGAGTACCTGGCA CCACTGCGCGCTGTGGACCTCAAGCTGTACCATGACCCCGACCTTCTGTTGGGCCCCGGTTGTGTGTACC CCGCTGCCTCTGTGGCTCGCTTTGCCTCACACTGGCGCCTTCCCCTCCTGACTGCGGGGGCAGTGGCCTC TGGCTTTGCAGCTAAGAATGAGCATTATCGTACCCTGGTTCGCACTGGCCCCTCTGCGCCCAAGCTGGGT GAGTTTGTAGTGACATTGCACGGGCACTTCAATTGGACAGCTCGGGCTGCTTTGCTGTATCTGGATGCTC GCACAGATGACCGGCCCCACTACTTCACCATCGAGGGCGTCTTTGAGGCCCTGCAGGGCAGCAACCTCAG TGTGCAGCACCAGGTGTATGCCCGAGAGCCAGGTGGCCCTGAGCAAGCCACCCACTTCATCAGAGCCAAC GGGCGCATTGTGTATATCTGCGGCCCCCTGGAAATGCTGCATGAGATCCTGCTTCAGGCTCAGAGGGAGA ACCTGACCAATGGGGACTATGTCTTCTTTTACCTTGATGTCTTTGGGGAGAGTCTCCGAGCAGGCCCCAC CCGTGCAACAGGCCGGCCATGGCAGGACAATCGAACCCAGGAACAGGCCCAGGCCCTCAGAGAGGCCTTT CAGACTGTATTGGTGATCACATACCGAGAACCCCCAAATCCTGAGTATCAGGAGTTTCAGAATCGCCTGC TGATCAGAGCCCGGGAAGACTTTGGTGTGGAGCTGGCCCCATCCCTGATGAACCTTATTGCTGGCTGCTT CTATGATGGGATCCTGCTCTATGCCCAAGTCCTGAATGAGACTATACAGGAAGGGGGTACCAGGGAAGAT GGACTTCGAATTGTGGAGAAGATGCAGGGACGAAGATACCATGGTGTAACTGGACTGGTTGTCATGGACA AGAACAATGACCGCGAGACTGATTTCGTCCTGTGGGCCATGGGAGACTTGGATTCTGGGGACTTTCAGCC CGCAGCCCATTACTCTGGAGCAGAGAAGCAGATTTGGTGGACAGGCCGGCCAATTCCCTGGGTGAAGGGG GCCCCACCTTTGGACAATCCCCCCTGTGCCTTTGACTTGGACGACCCATCCTGTGATAAAACTCCACTTT CCACTCTGGCAATCGTGGCCCTGGGCACGGGAGTCACCTTCATCATGTTTGGTGTTTCCAGTTTCCTAAT TTTCCGGAAGCTGATGCTGGAGAAGGAGCTGGCTAGCATGCTATGGCGCATTCGCTGGGAAGAACTGCAG TTTGGCAACTCGGATCGCTATCACAAGGGTGCAGGCAGTCGCCTGACGCTGTCGCTGCGGGGATCCAGTT ACGGCTCGCTCATGACAGCCCATGGGAAATACCAGATCTTTGCCAACACCGGTCACTTCAAGGGAAATGT TGTTGCCATCAAACACGTGAATAAGAAGCGCATCGAGCTGACCCGGCAAGTTCTGTTTGAACTCAAACAC ATGAGAGATGTCCAGTTCAACCATCTTACTCGCTTCATCGGAGCCTGCATAGACCCTCCCAACATCTGCA TTGTCACCGAGTATTGTCCTCGTGGGAGCTTACAAGATATTCTAGAAAATGACAGCATCAATTTGGACTG GATGTTTCGCTACTCGCTCATCAATGACCTTGTGAAGGGTATGGCCTTTCTCCACAACAGCATTATTTCA TCTCATGGAAGCCTCAAGTCCTCCAACTGTGTGGTGGATAGTCGATTTGTGCTCAAAATAACAGATTACG GTCTGGCTAGTTTCCGATCAACTGCTGAACCGGACGACAGCCATGCCCTCTATGCCAAGAAGCTGTGGAC TGCCCCAGAACTGCTTAGCGGGAACCCCTTGCCAACCACAGGCATGCAGAAAGCAGATGTCTACAGCTTT GCCATCATTCTACAGGAAATAGCACTTCGAAGTGGTCCTTTCTACTTGGAAGGCCTGGACCTCAGTCCCA AGGAGATTGTCCAGAAGGTGCGGAATGGTCAGAGGCCGTATTTCCGGCCAAGCATTGACCGGACACAACT GAATGAAGAGTTAGTTTTGCTGATGGAGAGATGCTGGGCCCAGGACCCAACAGAACGTCCAGACTTTGGG CAAATCAAGGGCTTCATTCGCCGGTTTAACAAGGAAGGTGGTACCAGCATATTGGACAACCTCTTGCTGC GCATGGAACAGTATGCTAATAACCTGGAAAAGCTGGTGGAGGAACGCACACAGGCCTATCTAGAGGAGAA ACGCAAAGCAGAGGCCCTGCTGTACCAAATTCTACCCCATTCTGTAGCAGAGCAGTTAAAGCGGGGAGAG ACGGTTCAGGCGGAGGCCTTCGACAGCGTCACCATCTACTTCAGTGACATCGTGGGCTTCACAGCGCTGT CAGCTGAGAGCACCCCCATGCAGGTGGTGACACTTCTTAATGACCTTTATACCTGCTTTGATGCCATTAT CGACAACTTTGATGTCTACAAGGTGGAAACCATTGGGGATGCCTACATGGTGGTATCTGGCCTTCCAGGC CGAAACGGTCAGCGCCATGCACCAGAAATTGCTCGAATGGCCCTAGCATTGCTAGATGCTGTCTCTTCCT TCCGCATCCGCCATCGACCCCATGACCAGCTGAGGTTACGCATAGGTGTCCATACTGGGCCAGTCTGTGC TGGGGTGGTTGGCCTGAAGATGCCCCGGTACTGTCTTTTTGGAGACACAGTAAACACTGCTTCTCGAATG GAGTCGAATGGCCAAGCTCTAAAGATCCATGTCTCCTCGACCACCAAGGACGCCCTGGATGAGCTGGGAT GCTTCCAGCTCGAGCTCCGTGGGGATGTGGAGATGAAGGGAAAAGGAAAAATGCGAACTTACTGGCTCTT GGGAGAGCAAAAGGGACCTCCCGGACTCCTGTAAAGCCCAGGCCGGCCATCTTCTGCTGCTGGTACTTGG ATGGGCAATGGTCACTGTGTTTCCACACAGCAAAAACAGACGTAGCCATATCAGATGGAAAACATGCATA GACACAAACCCCCTGCCTTATGTGGAAATCATAGCCCCCTGCCGCTCAGCCTTGTACATAGACCTATCCC TCCCTGCCCTGGTCCTCTTCCTGCCTCCTGTAAATATCTGTATCTAGACCAGAATATTTTGTCCAAGTAT AAAACAACAACAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5137261 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5137261 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14694 same embryo
	EMAGE:14695 same embryo
	EMAGE:14697 same embryo
	EMAGE:14693 same embryo
	EMAGE:14696 same embryo
	EurExpress:euxassay_005611 same experiment
	MGI:4826771 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source