EMAGE mouse gene expression spatial database

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EMAGE:14694

Cbx2 chromobox homolog 2 (Drosophila Pc class) ( MGI:88289)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005608. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005608_01	euxassay_005608_02	euxassay_005608_03	euxassay_005608_04

euxassay_005608_05	euxassay_005608_06	euxassay_005608_07	euxassay_005608_08	euxassay_005608_09

euxassay_005608_10	euxassay_005608_11	euxassay_005608_12	euxassay_005608_13	euxassay_005608_14

euxassay_005608_15	euxassay_005608_16	euxassay_005608_17	euxassay_005608_18	euxassay_005608_19

euxassay_005608_20	euxassay_005608_21	euxassay_005608_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14694_wholemount_strong.wlz

14694_wholemount_moderate.wlz

14694_wholemount_weak.wlz

14694_wholemount_possible.wlz

14694_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14694_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4979
Entity Detected:	Cbx2, chromobox homolog 2 (Drosophila Pc class) ( MGI:88289)
Sequence:	sense strand is shown >T4979 GGCGGGGCGCGGCGGGGGCGGCGCTTTGTGTGCAGCAGTGAGCCGGGGTCTGCGGGGCGCGGGCCGCGGC GGGCGGCGGCATGGAGGAGCTGAGCAGCGTGGGCGAGCAGGTCTTCGCCGCCGAGTGCATCCTGAGCAAG CGGCTCCGCAAGGGCAAGCTGGAGTACCTGGTCAAGTGGCGCGGCTGGTCCTCCAAACACAACAGCTGGG AGCCAGAAGAGAACATTTTGGACCCGAGGCTGCTCCTAGCCTTCCAGAAGAAGGAACATGAGAAGGAGGT TCAGAACCGGAAGAGAGGCAAGAGACCCAGGGGCAGGCCGAGGAAACACACAGTCACATCCTCCTGCAGC CGGCGCTCCAAGCTCAAGGAACCAGATGCGCCATCCAAATCCAAATCCAGCAGTTCGTCCTCTTCCTCCA CATCTTCCTCCTCTTCCTCGGACGAAGAGGAAGACGACAGCGACCTAGACTCCAAGAGAGGCCCCCGGGG CCGTGAAACCCATCCAGTGCCTCAGAAAAAAGCCCAGATCCTGGTAGCCAAGCCAGAGCTGAAGGATCCC ATTAGAAAGAAACGGGGACGCAAGCCTCTACCCCCAGAACAGAAGGCAGCTCGGAGACCCGTCAGCCTGG CCAAGGTGCTAAAGACCACCAGGAAGGATCTGGGGACCTCAGCCGCCAAGCTGCCCCCTCCACTCAGCGC TCCGGTGGCAGGCCTGGCTGCCCTGAAGGCCCACACCAAAGAGGCCTGTGGTGGCCCCAGCACTATGGCG ACCCCAGAGAACCTGGCCAGTCTGATGAAAGGCATGGCCGGGAGCCCCAGCAGAGGCGGCATCTGGCAGA GCTCCATCGTACACTACATGAACCGCATGAGCCAGAGTCAGGTTCAGGCTGCCAGCCGACTGGCACTCAA GGCCCAGGCCACCAACAAGTGCGGTCTCGGGCTAGACCTGAAAGTGAGGACGCAGAAGGGGGGTGAGCTA GGGGGGAGCCCCGCAGGAGGCAAGGTCCCGAAGGCCCCCGGTGGCGGAGCTGCAGAGCAGCAGAGAGGGA ACCATTCGGGGAGCCCAGGTGCTCAGCTGGCACCCACTCAGGAGTTGAGCCTTCAGGTCCTTGACTTGCA AAGCGTCAAGAACGGTGTGCCTGGTGTGGGCCTGCTTGCTCGCCATGCCCCAGCCAAGGCTATTCCTGCT ACCAACCCAGCCACAGGGAAAGGTCCTGGGAGCGGCCCCACAGGAGCAAACATGACCAACGCTCCCACAG ACAACAACAAAGGGGAAAAGCTGACTTGCAAGGCAACGGCTCTGCCTGCCCCTTCCGTCAAGCGGGACAC CGTTAAAAGCGTCGCTGCCTCCGGCGGGCAGGAGGGCCACACAGCCCCGGGAGAAGGCCGAAAGCCACCT GCGCTGTCTGAGCTGAGCACGGGAGAGGAGAATAGTAGCTCTGACTCGGACCCTGACTCGACCTCGCTTC CCAGTGCTGCGCAGAACCTATCTGTAGCTATCCAGACCAGCCAGGACTGGAAACCTACCCGCAGTCTCAT CGAACACGTCTTTGTCACGGATGTCACAGCCAACCTCATCACTGTCACCGTGAAGGAGTCGCCCACCAGC GTGGGCTTCTTCAACTTGAGGCATTATTGAGACCATTGCCCGAGCCAGACCTGCCTTCCAGTCTCTGGTT TTGCTTTGATGCCTGACCTGGGGGCCAACTCTGCCCTGTCCTGAGACTCAGGGCTGGGAAATGGCTTCCT GCTGATTCTACCTCTCTGGGCCTCCACTTCAGTCTGTCCTGTCATCTCAGGAAAGCCGGGTGGCCACTGA CTTAAAAGCTACCTGCTTGGACATAGCATCCTTTCTACACATCCCTCCTTAACCTAGTGAGACCCTCCCC TCCCTGGCCAACTACTGTGAGGTGGATTGGGGAGGGGGGGGCACTGATACAGCTCCCTCCCCAGGCCGTT GAGGAGAGGGGTGGGGCTGGTAGGGCCTTCTCCCACATCCAGCCACGGCAGGGTCTTTCTGGTGAGGGAG ACACTCTATGCTTTGCAAGTAAGGCTATAACTTCAGTTTTAAAAGGCTAATTAGTTGTGTGGTTGGGCTC AGGGACCCTGCGGAAATCTTCAGTCTCTATCCGTGGTGTGGTGTCCCCTCTAGTTGCAATTGTAGTCCCA AAGCCCAGTCAGGGTTTGGAGTCCTGGCACTAAGGTGTACCTGGGTGCTTCTGGACTTGGGAATAGAGAG AGCCATGTCCTTATTGCCTCCTTCAAGCAGGCTCAAGCCCCAGTGAGAAGTGACCCTGTTCCTCTCTGGG TCTGGACCCATACCCCTGCTTTGGCCCACTAAATTGCACCCTAGTTCTGAGGTCTGACACTCCTGGGCTT GGGGAAGGAGCAGGCTAGACACCCAGAGCAAGCTGATGTAGGCACTTGGAGTCCTGGGCAAGCAATCTTC CTTCATGTTGGACTTTTATTACTAGGACCCCATAAGTCACCATGGTGGCACCAGTGTGGGAATGCAGGTG TTTTGTGGGGGCCTGTCACCCTGCTGCCTGTCTAGACATCTCTAAACTACTGTGGAAGCATTTTCTATAG TCAGTCTTGGTGCAGAGCTCCGCCTGGTGAGTGGGCCACTCTTGGGTCCCATATTCCCTGACAATCCTCT CCCACATTTGGGAGCTTGTGTTTCCTCCCTCGCTGAGAAGCAATAGCCCTATGCCATTTGTGTGGTGGGG TCGTTTAGGAGCCCACCTGGCAGGGGGCCCCTAATTTCTTTCCCATCCTAGGAGATAAGTCTAGTGCTGA GCCCCTTTCTAAATGCCTTCTCCCCTGTCCCCCATCCCGGGTAACTGTCTTGAAACTACCTTGTCAGATT GCTGGCCGATGACAGGATTTTCTGTCCTGGGATATCACGGTGGGCCGTGACAATCTGGTCAGTGAGTCTG TGATGCCAAAAAGTAGGGGTGCTATGAAACCCTGGTTCTGCTTCTGCCATTGACTGATCAGCCAGCAGGT GTGGGCTCTTCACTCCAGTTGCCTTGAAGGGAAATGTTTCCCCTTCTAAAACCAGTCAGACAGTGGGCTG AGGTGTCTGGGACCTGCCACACTTTCAAAGTGCACCCCACCCCCACCCCAGTAGCTCACCCTGCACACCA AATAAGATGGTCTTTGTGTAACCAAACCAACCTCAATGCTGTGCCTTTTGGGGCAGACCCATATCCACAG CTCCTGAGAGATGCCTGTGATGTGCCAGGGGACCATGTTACTGGCAGCAGGAGCTACGTACTGGGAAGAA TTCAGGGCCAGGAAGTGGGTAACAAAGGGGAAAGGGTTGCCTACTGCATTATCAATGTCCCTCCCTGCCC TCAGCCAGGGTCTGCAACCTTTGTACCAGACACTGCCTTTACAAACACCTCCCCGTCTCTTTGGGGTCCC ATGAGCTGTTGGAGAAGTGACCCTGTTTGGGGATCTGGGTTGACTGGCCTTTGTCCCCTGCTGCCAGTCT CCAAATCCCAATTTGGTGCCTTTTATTTTACTGGTTACGTCAGTCCCAAAGTTGAAATCTACAAATCCTT TCCTCTCAGCCACTTTGCCTTCTTGTGTGTTTTTCCTGGTGCTGTGACCCCAGTGGTCTATGTCACAAAG CACATGTGTGTAGTGCGCCAGCGCGAGCCCCACTGGAGCAGCGCCATTCGCTCTGAGTCAAGTTCTCGTG TTTACCGATAAATAAAGTAGTTGGTTTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5137505 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5137505 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14695 same embryo
	EMAGE:14692 same embryo
	EMAGE:14697 same embryo
	EMAGE:14693 same embryo
	EMAGE:14696 same embryo
	EurExpress:euxassay_005608 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source