EMAGE mouse gene expression spatial database

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EMAGE:14695

Mast3 microtubule associated serine/threonine kinase 3 ( MGI:2683541)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005610. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005610_01	euxassay_005610_02	euxassay_005610_03	euxassay_005610_04

euxassay_005610_05	euxassay_005610_06	euxassay_005610_07	euxassay_005610_08	euxassay_005610_09

euxassay_005610_10	euxassay_005610_11	euxassay_005610_12	euxassay_005610_13	euxassay_005610_14

euxassay_005610_15	euxassay_005610_16	euxassay_005610_17	euxassay_005610_18	euxassay_005610_19

euxassay_005610_20	euxassay_005610_21	euxassay_005610_22	euxassay_005610_23	euxassay_005610_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14695_wholemount_strong.wlz

14695_wholemount_moderate.wlz

14695_wholemount_weak.wlz

14695_wholemount_possible.wlz

14695_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14695_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4976
Entity Detected:	Mast3, microtubule associated serine/threonine kinase 3 ( MGI:2683541)
Sequence:	sense strand is shown >T4976 CGGACGCGTGGGTCGGCCGGCTGAGCGCTGACCTCCGGCTAAGGTCCTGGACACCTGCCTCCTCCTGTCA GCCATCTTCCTGTCAGACTGACCGGGGTCCTAGCCCGTCTCTCCTAAGCACTATCAGTTTGGATGTGATG CCTAAATTTGCCTTCTCCTCTGAAGATGAGGGAGCAAGTTCAGGACCTGCTGATCCCCAAAAGCCTGTCT TCATTCTGGGGGAGCCTGACCCCCCACCCCCGACCACCCCAGTGACACCCAAGCCCTGCAACCTTTCTGC TGACACAGCTGTCCTCAGCCATGCCCGCCTTCGAAGTAACAGTACAGGAGCCCGGCACTCCACTCCTCGG CCCCTGGATGCTGGCAGAGGCCGCCGCTTAGGAGGCTCAAGAGATCCTGGCCCTGAGAAGCCCCGGGCCT CCCCTGGTGGCAGTGGGGGTCGAGTGCCCAAGTCAGCCTCTGTGTCCGCCCTGTCCCTCATCATCACGGC AGACGACGGCAGCGGGGGCCCTCTCATGAGCCCCTTGTCGCCCCGCTCGCTGTCCTCCAATCCCTCCTCC CGAGACTCCTCACCCAGCCGGGACCCATCACCAGTGTGTGGTAGCCTGCGGCCTCCCATCGTCATCCACA GCTCAGGCAAAAAGTATGGCTTCAGCCTGCGCGCCATTCGTGTCTACATGGGTGATAGTGACGTGTACAC GGTGCACCATGTGGTCTGGAGCGTGGAGGAGGGGAGCCCTGCACAGGAGGCAGGACTGCGTGCTGGGGAC CTCATCACACACATCAATGGGGAGTCGGTCCTGGGCCTGGTACACATGGACGTGGTGGAGCTGCTGCTGA AGAGTGGCAACAAGATCTCCCTGCGCACCACGGCCCTGGAGAACACCTCCATCAAGGTGGGACCCGCGCG GAAGAACGTGGCCAAGGGCCGCATGGCCCGTAGGAGTAAGCGCAGCCGCCGGCGTGAGACCCAGGACCGG CGGAAGTCTCTGTTTAAGAGGATCTCAAAGCCATCTTCTGTGCTCCACACCAGCCGTAGCTTTTCCTCAG GCCTCCAGCACTCTCTGTCCTCCAGCGAGAGCCTCCCCGGATCACCCACTCACAGCCTGTCTCCCAGCCC CACCACGCCCTGCCGCAGCCCTGCCCCCGACGCCCCCACAGGTGGGCGGGCTCTCAGCCTGGCTTTATGA AACTCTGCCTGTGGATATGAATGTATACGCACGCCTGTGATAACAATCAACACAGCATCCCCACCCAATG TGTCCCCAAGCTCCAGCAGCCCCGCTTCTCCAGCCACTGGTCACACCCGTCCCAGCTCCCTGCATGGCCT GGCAGCCAAGCTCGGACCACCTCGTCACAAGAGCGGTCGCCGCAAGTCCACAAGCAGTATCCCTCCATCT CCGCTGGCCTGCCCGCCCGTGCCCACGCCCCCACCACGCTCACCATCCCCCCTTCCGGGGCACATCCCCA TACCCGCCCGGTCTCCTAGGCTGCGTAGGGGCCAGTCAGCCGACAAGCTGGGCCTAGGCACCAGTGAGAG GCTGGATGGGGATGGAGGCCGGCGCGCCCGGGGGGCCGAGGCTGAGCTGGTCGTCATGAGGAGGCTGCAT CTATCAGAGCGTCGGGATTCCTTCAAGAAGCAGGAGGCAGTGCAGGAGGTGAGCTTCGATGAGGAGCCTG GGCCGCCTCGCGGGGTGCCGAAGATCGCTGTGCAGGGTGCAGAGGCCACACCTGGAACCCCAGGACATGC CAGGAAAGACTGACCACCTCTGGCTGCAGTTCCTTATGCAGCTATCATCTTCACCAAGTCACACCTAGAT GGGGTGCAGCTATCAGCCTGGGACTCAGCCGAGGGTGCCCCCTTCCCAGGAAGGGCTGGGATGGGGGTGC CAGTGACTTGTAAATAGCAAGCCCTCGCGACTAATTTATTACTTTTTATAAGCAATAGCCAGGCTGGCCC GCCTGGTGGACTCGGCTGTCTTGTCCTTGGCCCCTGGAGCACAGCCTACAGACCCTGTTCTCTGGGTCAA GGCAAAGCACTTTGGGCAACTTCTGTGTTTTCTAGTTTGTCCTTTCTGTGTGTGTGTGTGTGTGTGTGTG TATGTGTATGTCCATCCCATTCCCAACCCAAGACCCACTTGAAGGCACACAAAAGCTGGTCCCTACTGAG TTTTGACAGAATTTGGAAGCTGCACCCTAAAGCAGGGGGATCACCAGTTCTGTGGCAGGCTATACTCTGA AGACTACCTTCTGTGTCTGAATAGCTCTCCTCAGACTGTCCTTACTCCACTCTGCCTGTGATCCTGCATG CTTCAGTTTAGGGGAAGTATGGGAACAAAGGACTTGGGGGATGAGGAATGCTCTGGTTTGAGGCAGAATA GCCCATATACCTTGCAGGAAGTAACTGACAGACCTAAGGGCCATCAAGGTGGGGAAGGTGTCCACTGCAG GGTGGGACAAAGCACTTGTCCCAAGTGAGGACCATGCCTGACCACCAACCCCTGCACCACAAAGTCTATG CCCATATGCACGAGGCCCAGCATGGTGAGTGCCAGAGGACCGAGTGTGGACCCCAGCTATGCTGTGTAAT TCTAGAGGAGTGTCTTTCCCATTCTGTGCCTCTTTACAGGACTAACCCCAGTGACTTGCACACTTCATGT GCACTGAGCACACCTGAATTCCCCCCAGCTTTGTGTGATCCAAGAAGCTGAGGTTCCTAAGGCCAGAGGC ATGTCCCACAATGGCCCAGTAAGAGTGGCAGCCCAGGACAGGCTCTCCTAGCCCTACTGTGAGCATGGCC ATGGGGGAGCCCAGACTCCCATGCTGTGTGATCTAGCAGCCATGTAGTAGGTTGCCAGTGGTGCAGCTCT ACACACTCCACGAGGGGAATTCGCAGGCACTCAAACCCTCTGCACCTCCCTGACTCGCAGCCAGTACCTT CTGCTGGAAGGGAGACCAAGGCTGTGCATGAGTAGCCCTCTACCCATCTTGGAGCTGGCATCAAATAAAG TCCCATGAAGACATCTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5137303 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5137303 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14694 same embryo
	EMAGE:14692 same embryo
	EMAGE:14697 same embryo
	EMAGE:14693 same embryo
	EMAGE:14696 same embryo
	EurExpress:euxassay_005610 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source