EMAGE mouse gene expression spatial database

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EMAGE:14696

Slc25a32 solute carrier family 25, member 32 ( MGI:1917156)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005621. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005621_01	euxassay_005621_02	euxassay_005621_03	euxassay_005621_04

euxassay_005621_05	euxassay_005621_06	euxassay_005621_07	euxassay_005621_08	euxassay_005621_09

euxassay_005621_10	euxassay_005621_11	euxassay_005621_12	euxassay_005621_13	euxassay_005621_14

euxassay_005621_15	euxassay_005621_16	euxassay_005621_17	euxassay_005621_18	euxassay_005621_19

euxassay_005621_20	euxassay_005621_21	euxassay_005621_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14696_wholemount_strong.wlz

14696_wholemount_moderate.wlz

14696_wholemount_weak.wlz

14696_wholemount_possible.wlz

14696_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14696_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4989
Entity Detected:	Slc25a32, solute carrier family 25, member 32 ( MGI:1917156)
Sequence:	sense strand is shown >T4989 CGGACGCGTGGGCGGGCTCCGGTCCCGGAAGTGGGCTCGCGTCCTGCGGTTCCAGGCCCCGGGCCGCGAC AGTCGCCACGGTGGCGGCCACTACATCACGCCCCACGTCGGCGGCGCTGGACGCCGTAGTGTCGACGAAC CCGGAGCCCATGACAGGCCAGGGCCAGTCGGCTGCCGGGTCGGCGGCGTGGAGCGCGGTGTTCCGCCACG TCCGGTACGAGAACCTGGTGGCTGGCGTGAGTGGCGGGGTCTTGTCCAACCTGGCGCTGCACCCGCTCGA CCTCGTGAAGATCCGCTTCGCTGTGAGTGATGGACTGGAAGTAAGACCAAAATATAAAGGAATTTTGCAT TGCTTGGCTACCATTTGGAAAGTTGATGGACTACGAGGACTTTATCAAGGAGTAACCCCGAATGTGTGGG GTGCCGGTTTATCCTGGGGACTCTACTTTTTCTTTTACAATGCCATCAAATCGTATAAGACAGAGGGAAG AGCTGAACAGTTAGAGCCATTAGAGTACCTCGTCTCAGCTGCTGAAGCTGGAGCCATGACTCTGTGCATT ACAAACCCATTATGGGTGACGAAAACTCGCCTTATGTTACAATATGGTGGTGTTGCTAGCCCTTCACAGA GACAGTATAAAGGAATGTTTGATGCACTTGTGAAAATATATAAATATGAAGGCGTGCGTGGATTATACAA GGGATTTGTCCCTGGGCTGTTTGGAACATCACATGGTGCCCTTCAGTTTATGGCATATGAGTTGCTAAAG TTGAAGTACAACAAACACATCAATAGATTACCGGAAGCCCAGCTGAGTACAGCAGAATACATCTCTGTCG CAGCGCTATCCAAAATATTTGCCGTAGCAGCAACATACCCGTATCAGGTTGTGAGAGCCCGCCTTCAGGA TCAGCATGTGTCTTATGGTGGTGTAACAGATGTGATCACAAAGACGTGGAGGAAAGAAGGCATCGGTGGA TTTTACAAAGGAATTGCCCCCAATCTGATTAGAGTGACTCCAGCCTGCTGCATCACCTTTGTGGTTTATG AAAATGTCTCTCACCTTTTATATGACCTTAGAGAAAAGAAAGTGAGCTAAAAGAAGATCCCAGTCTATGT TCTTGAGGCAACAGCAAAGTCCTTTGTGTTTGAAGCGCAAAGCTCAGAAGAGTTCTGCAGAGCTACATAG TTGGTAACCCCATGTCTGTGTTACAAGTGAAAGAACTGCTAAGTCATCTTGATGTCTTCTGCTCTTTGCA TCTCCGTGTGTGTGGAGCTTGGCAGCCTCTACCTGGAGTGGGTACCATCTGCCTGACGTTGCAACTGATA GCCACGGCAAAAATGTACAGGATCTTCCATTTTGTTATTCAGGGAGAAGCTCATTTGCAATATTTGTAAA TAATTTGATGAATGTTCTTTTTTTCTGAACTCTCTTGCCTGGATTGGCTTTAAAACTGACCATGTGCAAT AGCAAGGAACTGGCTTCTTACAAGAATGACTATATTTCAAGGGTAAATTAAACTTGGGGAACTTATAATA AGGTATTATCTAACTCTAGCCCATTTTGAATGGGGATATTTTTGTTAGAAAGAAGTCTGTTATAGACGTG CTTTAGTTTTAATCCAACTGGAACATACTAAGTACAGAAATTGGAGAAGTCATGGATTTCTCTGAGAAAT ATCTAGCACCTTTTGCCATTCCATTGCTTAATTATTTACAGTGAGATTCTGGACGCAGACCTTGTATTTT GGCCATAAACACTTAGTGTGTGGTTAGGGTGTATAAAACCTGAACTTGCAGGATCCGGTGAAGCCTTTAT TTAGCATGTGTGGCTACAAATCACTCATCAGAAATGCTGGCTCATGTTTCACAGAGATGGAATGTTTGAT GTAAGCTTGTTGAAAAACTGAATAGAAACAAGCATTCTAGTTTCCCTTCATTTTTAGATTTATGGTTCCA GATTCCAAATAATTATTTGTCTGCATTGGTTGCTCAAATTCAGGTTGAAATACAAATTACATTTGTCTGT GGCCATTACTGGTTTGATTTCTAAGTCATTGTAGATCTCCAGGTAAAAGGCCTGCTCCCGAAGGATGCTA GACTAATACTAACTCTTCTGATGAGCAACTGTCAGTACTGTTGCACTAGCCTCTATTTTTTGTAAATAAA TCATTATAATCAAAGCTGTATTTAAAGGGTTCTTTGAAAATAAGCCTATTTTTTTATATTTTGCAAATGG AATTGTTTTTAAAATAAAAAATGAAAAGATAATTTATATTTACAAAAATGGTTTTGCTATAATATCCTTT ACCTTTATTTTTTAAGTATGTTTGTTTATTCAGCAAAATTGATACATTTTAAAATCACGATACACTGTAA CAAAAAGCAAGTTTTTCAGCATTTACAAATTCCTGGCATATAACTCTTTGAAATTTGGGGACAATGAATC AATATTTCCAGGAATTCTTAGAAATTTCAAAATTACTATTTTAAACACAGTCATGACTATATCTACACTT TATTTGGGTATCTCAAATTTTTGTTACAGAATAAAATACACTGAATTTAGTGTAAGTTAGTTACTTATAT AGTAAGTAAATACAAAGAACTACAGCCTATAGGAATATTTAAACAATGAGAAATATAATTTGCAATTCAG ATCTCCCAGGTAAATAACTTCAGAATGTTTTCTAAATTATGCATTTCGTAGTTGCATGGGGTTCACCTTG ATACTTATGTCTGTATGGGAAGGCAAGCTATGGTTGATATTAAAGTTTTAGAAGTAGAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5149984 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5149984 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14694 same embryo
	EMAGE:14695 same embryo
	EMAGE:14692 same embryo
	EMAGE:14697 same embryo
	EMAGE:14693 same embryo
	EurExpress:euxassay_005621 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source