EMAGE mouse gene expression spatial database

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EMAGE:14697

Wdr24 WD repeat domain 24 ( MGI:2446285)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005616. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005616_01	euxassay_005616_02	euxassay_005616_03	euxassay_005616_04

euxassay_005616_05	euxassay_005616_06	euxassay_005616_07	euxassay_005616_08	euxassay_005616_09

euxassay_005616_10	euxassay_005616_11	euxassay_005616_12	euxassay_005616_13	euxassay_005616_14

euxassay_005616_15	euxassay_005616_16	euxassay_005616_17	euxassay_005616_18	euxassay_005616_19

euxassay_005616_20	euxassay_005616_21	euxassay_005616_22	euxassay_005616_23	euxassay_005616_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14697_wholemount_strong.wlz

14697_wholemount_moderate.wlz

14697_wholemount_weak.wlz

14697_wholemount_possible.wlz

14697_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14697_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4982
Entity Detected:	Wdr24, WD repeat domain 24 ( MGI:2446285)
Sequence:	sense strand is shown >T4982 CCACGCGTCCGGTGCGACGGGGCAGAGGCTTTTCTAGCACCTGGTTCTGGTCCCTCAGTTGCCGCTGCAC GGTGCAATTAGACACCTGCACTTATTGCTGTAGCCTCTCTTCTGCATTCCAAGGTGTATGAACTCAGTCC ATACCCATCTAGCTTCTCTGCCCTTTCCACTCGGTTGTTTTTCCTCTTGGAAGCCCCGCAATCCTCGGAG GCATGGGGCTGTGAAGTCAGGAGCCACTCCCCGCCCCCAGGATCTCACTGATGGAGAATTAACCACATAG AATCGGGAGACGTCAGATATGGTGCTGACGAACCTCAGGTTAGCTCTGGAGCCTTCTGGAGAACTCCAAT GAATGTGAACCTTCCTAAGATAACGACTGGCTGATGGACCATCTCAGGCCGAAGGTTCGAGAGGAGACTG AACCTGAATTGTTAGGACACCATGGCTGACCAGGTCAGCACACTTCACTGACCTCCTCCTGCCTCTGATA CCTGTGCAGACATGGAAAAGATGTCCCGAGTTAGCACAGCCCTGAGCGGCAGTGCTCTGACGGGCCGAAC CATGCACTGCCATCTGGACGCGCCAGCTAATGCCATCAGCGTGTGCCGCGACGCTGCCCAGGTTGTCGTG GCAGGCCGAAGCATCTTCAAGATTTATGCCATTGAGGAGGAGCAGTTCGTAGAGAAGCTGAATTTACGTG TGGGTAGGAAGCCCTCGCTCAACCTGAGCTGTGCTGATGTGGTCTGGCACCAGATGGATGAGAATCTGCT GGCCACAGCAGCCACCAATGGTGTGGTGGTTACATGGAACCTAGGTCGGCCATCCCGCAACAAGCAGGAT CAGCTGTTCACAGAACACAAGCGCACAGTGAACAAAGTCTGTTTCCATCCCACCGAGGCCCATGTGCTGC TCAGTGGCTCTCAGGATGGCTTCATGAAGTGCTTTGACCTCCGAAGGAAGGACTCTGTCAGCACCTTCTC TGGCCAGTCTGAGAGCGTGCGGGATGTGCAGTTCAGTATCCGTGACTACTTTACCTTTGCTTCCACCTTT GAGAATGGCAACGTACAGCTCTGGGACATCCGCCGACCCGACCGCTGTGAGAGGATGTTCACAGCCCACA ATGGGCCTGTCTTCTGCTGTGATTGGCACCCAGAGGACAGGGGCTGGCTGGCCACGGGTGGACGTGACAA GATGGTAAAGGTCTGGGACATGACCACACATCGTGCCAAAGAGATACACTGTGTGCAGACAATCGCTTCA GTGGCCCGAGTCAAGTGGCGGCCTGAATGCCGCCACCACCTGGCCACGTGTTCCATGATGGTGGACCACA ACATCTATGTATGGGATGTACGCCGCCCCTTCGTGCCAGCTGCTATGTTTGAAGAGCATCGTGATGTCAC AACAGGCATTGCCTGGCGTCATCCGCATGACCCCTCTTTCCTGCTCTCCGGCTCCAAGGACAGCACGTTA TGCCAGCACCTGTTTCGGGATGCCAGTCAGCCTGTTGAGCGTGCCAACCCTGAGGGTCTCTGCTATGGCC TCTTTGGGGACCTGGCCTTTGCTGTTAAGGAAAGCCTGGTGGCTGCTGAATCTGGACGTAAACCCTATGC TGGTGACCGGCGCCACCCCATCTTCTTCAAGCGCAAGTTGGACCCTGCAGAACCTTTTTCTGGCCTCGCT TCCAGTGCACTGAGCGTCTTTGAGACGGAGTCTAGCGGTGGCAGCATGAGCTGGTTCGTGGATACAGCTG AACGTTATGTTCTGGCTGGCCGGCCACTAGCCGAGCTCTGTGATCACAATGCAAAAGTGGCTCGGGAGCT TGGCCGCAATCAGGTGGCACAGACATGGACCATGTTACGGATTATCTACTGTAGCCCTGGCTTGGTATCC TCTGCCAATCTCAACCACAGCGTCGGCAAAGGCAGCTCCTGCGGCCTACCGCTCATGAACAGCTTCAATC TGAAGGATATGGGCCCGGGGCTGGGCAGTGAGACACGGCTGGATCGCAGCAAAGGGGACACACGGAGTGA TGCGGCGCTGCTTGACTCTTCAGCCACACTCGTCACCAATGAGGATAATGAGGAAACAGAAGGGAGTGAT GTGCCTGCTGACTACCTGCTGGGTGACGTAGAAGGAGAGGACGATGAGCTGTACCCACTTGACACAGAAC ATGTGCACTCCGAGGAGCCTGAATATGTGCTGCCACAAGAGGCCTTCCCACTGCGCCATGAAATTGTAGA CACACCATCTGGGCCCGAGCATCTGCAGGACAAGGCGGACTCTCCGCACGTGAGCGGCAATGAGGCAGAC ACAGCTTCATTAGCCCCGGTGGACTCCTCCTCCTCCCTCCTCTCCGTCTCACATGCACTCTATGACAGCC GCCTGCCACCTGACTTCTTCAGTGTATTGGTACGGGACATGTTGCGCTTCTACGCTGAGCAGGGCGACGT GCAGATGGCTGTGTCTGTTCTCATCGTGCTGGGTGAGAGAGTGCGAAAGGACATCGATGAGCAGACCCAG GAGCACTGGTACACATCCTACATAGATCTGCTGCAGCGCTTCTGCCTCTGGAATGTGTCCAATGAGGTGG TGAAGCTGAGCACCAGCAGGGCAGTTAGCTGCCTCAACCAAGCCTCCACCACGCTGCATGTCAACTGTAG TCACTGCAAGCGGCCCATGAGCAGCCGCGGCTGGGTCTGTGACAGGTGCCACCGCTGTGCCAGCATGTGT GCTGTCTGCCATCATGTGGTTAAAGGCCTGTTCGTGTGGTGTCAGGGTTGCAGTCATGGTGGCCATCTTC AGCACATCATGAAATGGCTGGAAGGCAGCTCCCACTGCCCTGCAGGCTGCGGCCACCTCTGTGAATACTC CTGACCTGGCCTTCCCTAAGGCTGATGCAGCGTCCTCAGCTCGATCCCGATGTCCACCGGACTAGGCTCA CAGCCCCTTACCCATCCTGTGTACCAGGATGGAAGGTGGCTACAACTTTTTAACCCAATAAAAATCCAGA GCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5148210 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5148210 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14694 same embryo
	EMAGE:14695 same embryo
	EMAGE:14692 same embryo
	EMAGE:14693 same embryo
	EMAGE:14696 same embryo
	EurExpress:euxassay_005616 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source