Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14711

Klhl12 kelch-like 12 (Drosophila) ( MGI:2385619)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14711 EMAGE:14711 EMAGE:14711 EMAGE:14711 EMAGE:14711
"Pseudo-wholemount" of euxassay_005662. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005662_01 euxassay_005662_02 euxassay_005662_03 euxassay_005662_04
EMAGE:14711 EMAGE:14711 EMAGE:14711 EMAGE:14711 EMAGE:14711
euxassay_005662_05 euxassay_005662_06 euxassay_005662_07 euxassay_005662_08 euxassay_005662_09
EMAGE:14711 EMAGE:14711 EMAGE:14711 EMAGE:14711 EMAGE:14711
euxassay_005662_10 euxassay_005662_11 euxassay_005662_12 euxassay_005662_13 euxassay_005662_14
EMAGE:14711 EMAGE:14711 EMAGE:14711 EMAGE:14711 EMAGE:14711
euxassay_005662_15 euxassay_005662_16 euxassay_005662_17 euxassay_005662_18 euxassay_005662_19
EMAGE:14711 EMAGE:14711
euxassay_005662_20 euxassay_005662_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14711Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14711_wholemount_strong.wlz
14711_wholemount_moderate.wlz
14711_wholemount_weak.wlz
14711_wholemount_possible.wlz
14711_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14711_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T942
Entity Detected:Klhl12, kelch-like 12 (Drosophila) ( MGI:2385619)
Sequence:sense strand is shown

>T942
TCCTCAGNCTGTTGGCCTACTGGACTCCGAGACTGGAGTTTACCACGGAGACTGCTCCGCAGCGGCTGGT
CCGGGCGGCAGCGGCGGCGCTCCGGCTGCTCAGCCGCCTGGGAGCCCGGCGCCTAGCACCTCGGCTCGGA
CGGCCCAGGAGCCCTGTGCTTGACGGGCGGCATTATGGCCCCTAAAGACATAATGACAAACACTCACGCT
AAGTCCATCCTCAACTCCATGAACTCCCTCAGGAAGAGCAACACCCTCTGTGATGTGACCTTGAGAGAGG
AGCAGAAAGACTTCCCTGCGCATCGGATTGTGCTGGCCGCCTGCAGTGATTATTTCTGTGCCATGTTCAC
TAGTGAGCTTTCAGAGAAGGGGAAGCCGTATGTTGACATTCAAGGTTTAACTGCTGCTACCATGGAGATC
CTGCTGGACTTCGTGTACACAGAAACAGTACATGTGACAGTGGAGAATGTTCAAGAACTGCTCCCTGCAG
CCTGTCTTCTTCAGCTGAAAGGTGTGAAACAAGCCTGCTGTGAGTTCTTAGA
Notes:The probe template was PCR amplified from IMAGE:1969968 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1969968 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14712 same assay
 EMAGE:14715 same embryo
 EMAGE:14713 same embryo
 EurExpress:euxassay_005662 same experiment
 EMAGE:14714 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS