EMAGE mouse gene expression spatial database

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EMAGE:14716

Gatm glycine amidinotransferase (L-arginine:glycine amidinotransferase) ( MGI:1914342)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005674. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005674_01	euxassay_005674_02	euxassay_005674_03	euxassay_005674_04

euxassay_005674_05	euxassay_005674_06	euxassay_005674_07	euxassay_005674_08	euxassay_005674_09

euxassay_005674_10	euxassay_005674_11	euxassay_005674_12	euxassay_005674_13	euxassay_005674_14

euxassay_005674_15	euxassay_005674_16	euxassay_005674_17	euxassay_005674_18	euxassay_005674_19

euxassay_005674_20	euxassay_005674_21	euxassay_005674_22	euxassay_005674_23	euxassay_005674_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14716_wholemount_strong.wlz

14716_wholemount_moderate.wlz

14716_wholemount_weak.wlz

14716_wholemount_possible.wlz

14716_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14716_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 21 moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 22 23 24

shoulder

moderate

regional

moderate expression: see section 01 02 03 04 22 23

upper arm

moderate

regional

moderate expression: see section 01 02 03 04 24

hand

moderate

regional

moderate expression: see section 08 09 24 weak expression: see section 22 23

foot

moderate

regional

moderate expression: see section 08 09 21 weak expression: see section 22 23

lower leg

moderate

regional

moderate expression: see section 01 02 03 04 05

upper leg

moderate

regional

moderate expression: see section 01 02 03 04 05 06 21 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 21 moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 22 23 24

frenulum

moderate

regional

moderate expression: see section 12 13 14

tongue muscle

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 12 13 15 16 weak expression: see section 10 11 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6144
Entity Detected:	Gatm, glycine amidinotransferase (L-arginine:glycine amidinotransferase) ( MGI:1914342)
Sequence:	sense strand is shown >T6144 CCCACGCGTCCGCCCACGCGTCCGGCGACTAGGACCTTGTGCACGCGGAGCAGCAGAGACGGGGCGATGC TACGGGTGCGGTGTCTGCGCGGAGGCAGCCGAGGTGCCGAGGCGGTGCACTACATCGGCTCTCGGCTTGG AGGATCCTTAACAGGATGGGTGCAGCGAACTTTCCAGAGCACCCAGGCAGCTACAGCTTCCTCCCGAAAT TCCTGTGCAGCTGAAGACAAGGCCACCCATCCTCTGCCCAAGGACTGCCCTGTCTCCTCTTACAACGAAT GGGACCCTTTAGAGGAAGTGATAGTGGGCAGAGCTGAAAATGCCTGTGTGCCACCATTCACAGTGGAGGT GAAGGCCAATACATATGAAAAGTACTGGCCATTTTACCAAAAAAATGGAGGTCTTTATTTTCCCAAAGAT CATCTGAAGAAGGCTGTTGCTGAGGTCGAAGAGATGTGCAATATTTTATCAATGGAAGGAGTGACCGTGA GGCGGCCTGACCCCATCGACTGGTCACTCAAGTATAAGACTCCTGATTTTGAGTCTACAGGTTTGTACAG CGCAATGCCTCGAGACATCCTGATGGTTGTGGGAAACGAGATTATAGAAGCACCCATGGCATGGCGCTCA CGCTTCTTTGAGTACCGAGCATACAGGTCAATTATCAAAGACTACTTCCATCGTGGTGCCAAGTGGACAA CAGCACCCAAGCCCACAATGGCTGACGAACTGTATGACCAGAATTATCCCATCCATTCCGTGGAAGACAG ACACAAATTGGCCGCTCAGGGAAAGTTCGTGACGACTGAGTTTGAGCCTTGCTTTGATGCTGCTGACTTC ATTCGAGCTGGAAGAGATATTTTTGCACAGAGAAGCCAGGTTACAAACTACCTGGGCATCGAGTGGATGC GTAGGCATCTTGCTCCAGACTACAGAGTACATATCATCTCTTTTAAAGACCCCAATCCAATGCACATCGA TGCCACCTTCAACATCATTGGACCTGGTCTTGTGCTCTCCAACCCCGACCGTCCTTGTCATCAGATTGAT CTTTTCAAGAAAGCAGGATGGACCATAGTTACTCCTCCAACACCAGTCATCCCCGATGATCATCCCCTCT GGATGTCATCCAAATGGCTTTCCATGAACGTCTTAATGCTAGATGAGAAGCGCGTGATGGTAGATGCCAA TGAAGTTCCAATTCAAAAGATGTTTGAGAAGCTGGGTATCAGTACCATTAAGGTTAACATTCGAAATGCC AATTCCCTGGGAGGTGGCTTCCACTGCTGGACCTGCGACGTCCGGCGCCGAGGCACCCTTCAGTCCTACT TTGACTGAGCAGGCGTGGTGTGGCTTGGGGCATAGCCTCAGATATACCTAAGAAGCAGCAAGGCCCTTCT CTCGCTTTAACAAATGCATGAACTGTAGTGCTTTGAACAATCACATCCTTAACAGGGTTTCCAAGCCTGG GTTACTTTTTGTTGACGTAAGGAAGATAACTTTTGATAGGTCTTACTTTCTTCTCCTAAAGTTATTTACG ATTTGGCTTTAAATATAAAAGTTCAGTAAGTGTATACCGAAGCAAAAATTAGTCACTTGAGTAACTTGGC CGCTAATATTTAACCATCTACCTCTGTTTTTAATTTTCTTTCCTAAAGGCAGCTTGAGATGTCGGTCCTA ACCTTGTCTTTTTTTTTTTCCTCTTTGGTAGACTTATGAATGTCCATATTACTTCTTTTTTCCTAAATGG GACAAAGACTTAGGCAAAAAACATGGATCCTAAGTAGAATCAGATATTTATCTCTTTTTCTAAAAAAATA AAATAAATAAATTTGGGTGTTTTTTTTTTTAATTTGCTTTTATCAGATATTTATCTCTTTTTCTAAAAAA ATAAAATAAATAAATTTGGGTGTTTTTTTTTTTTTTTAATTTGCTTTTAAACAACCTCGACTATGCAAAC ACTGAAAAAAAAAAAATGAAGTGACTTTCCATGAATGCCTTTAACATTCTTGTGTCAACATTTGGTACTA AACTCTTACTCTTACCTCATCTCAAGTGGGTTATCCTTCCTTCTGTCCTTCCTTCCGTCCTTCCCTCCTT CCTTCAGAGTAGCGCTTAGTGTAATAGAGTGAAACTTGTAGTTCCCATTCCTAAAGGTTGATGTTCAATA TCTAACAGGTATAATGGCATCTTCGGTAATCTAAGATTTGTTTACATATATGCAAAATTATTTAAGGTAT AGAGTTTTAAAGCATTTTTTTGGAAAAACTAATGCAGGTAAAACATGTGACATGAGATAATTTTAATGTT GGATATTATATATGTATTTTTATTCTAATAAACTTTTGTGTTCCAGATTGGAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3495871 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3495871 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14721 same embryo
	EMAGE:14718 same embryo
	EMAGE:14720 same embryo
	EMAGE:14719 same embryo
	EMAGE:14717 same embryo
	EurExpress:euxassay_005674 same experiment
	MGI:4825007 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source