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EMAGE:14720

Ppp3r1 protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I) ( MGI:107172)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14720 EMAGE:14720 EMAGE:14720 EMAGE:14720 EMAGE:14720
"Pseudo-wholemount" of euxassay_005677. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005677_01 euxassay_005677_02 euxassay_005677_03 euxassay_005677_04
EMAGE:14720 EMAGE:14720 EMAGE:14720 EMAGE:14720 EMAGE:14720
euxassay_005677_05 euxassay_005677_06 euxassay_005677_07 euxassay_005677_08 euxassay_005677_09
EMAGE:14720 EMAGE:14720 EMAGE:14720 EMAGE:14720 EMAGE:14720
euxassay_005677_10 euxassay_005677_11 euxassay_005677_12 euxassay_005677_13 euxassay_005677_14
EMAGE:14720 EMAGE:14720 EMAGE:14720 EMAGE:14720 EMAGE:14720
euxassay_005677_15 euxassay_005677_16 euxassay_005677_17 euxassay_005677_18 euxassay_005677_19
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euxassay_005677_20 euxassay_005677_21 euxassay_005677_22 euxassay_005677_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14720Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14720_wholemount_strong.wlz
14720_wholemount_moderate.wlz
14720_wholemount_weak.wlz
14720_wholemount_possible.wlz
14720_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14720_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6147
Entity Detected:Ppp3r1, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I) ( MGI:107172)
Sequence:sense strand is shown

>T6147
GGCGACGGCTAGATTTGTTGTGCTGTGTACATTCCCATGGGGTTGATGGGGTTTTGCCCTAGAGCATCGG
ATTGAAAATTAAAGTTTTGCTGCTTTCTTCCTTTGCTTTTTAGTTGATGCTGATGAAATTAAAAGGCTAG
GAAAGAGATTCAAGAAGCTTGATTTGGACAATTCTGGTTCTTTGAGCGTGGAAGAGTTCATGTCTCTGCC
TGAGTTACAGCAGAACCCTTTAGTACAGCGGGTAATAGATATATTCGACACAGACGGCAACGGAGAAGTG
GACTTCAAAGAATTCATTGAAGGAGTGTCTCAGTTCAGTGTCAAAGGCGATAAGGAACAGAAGTTGAGGT
TTGCTTTTCGTATCTATGACATGGATAAAGATGGCTATATTTCCAATGGAGAACTCTTCCAGGTGTTGAA
GATGATGGTGGGCAACAATCTGAAAGATACACAATTACAGCAGATTGTAGACAAAACCATAATAAATGCA
GATAAGGATGGAGATGGAAGAATATCCTTTGAGGAATTCTGTGCTGTAAGTGAATAGGCTGATTCATCTA
TTAACTCTTACCACTCATGAAGGACAGTTTACTAGACAGTTTTATAAGCATATTTTGCTTTGAAATTTTG
TCTAATTGCCCCACATTCAAGTTTTGGAATATGTTAGCTGGGCGTGGTAGTGCATGCCTTTAATCTCAGC
ACTCGGGAGGCAGAGGCAGGTGAATTTCTGTGTTCGAGGACAGCTATACAGAGAAACCCTGTCTCAAAAA
ACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3495820 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3495820 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14721 same embryo
 EMAGE:14718 same embryo
 EMAGE:14716 same embryo
 EMAGE:14719 same embryo
 EMAGE:14717 same embryo
 EurExpress:euxassay_005677 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS