EMAGE mouse gene expression spatial database

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EMAGE:14733

Pepd peptidase D ( MGI:97542)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005690. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005690_01	euxassay_005690_02	euxassay_005690_03	euxassay_005690_04

euxassay_005690_05	euxassay_005690_06	euxassay_005690_07	euxassay_005690_08	euxassay_005690_09

euxassay_005690_10	euxassay_005690_11	euxassay_005690_12	euxassay_005690_13	euxassay_005690_14

euxassay_005690_15	euxassay_005690_16	euxassay_005690_17	euxassay_005690_18	euxassay_005690_19

euxassay_005690_20	euxassay_005690_21	euxassay_005690_22	euxassay_005690_23	euxassay_005690_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14733_wholemount_strong.wlz

14733_wholemount_moderate.wlz

14733_wholemount_weak.wlz

14733_wholemount_possible.wlz

14733_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14733_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 08

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 14 15 16 17

tongue muscle

moderate

regional

moderate expression: see section 13 14 15 16

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

metanephros

moderate

regional

moderate expression: see section 06 07 08 09 15 16 17 18

lung

moderate

regional

moderate expression: see section 04 05 06 07 09 10 13 14 17 18 19 20 weak expression: see section 08 11 12 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2729
Entity Detected:	Pepd, peptidase D ( MGI:97542)
Sequence:	sense strand is shown >T2729 CTTGATCCCACAGTGTGATGATGCAGTCTCAGGGCCCTGAGCTTCAAAGGCAGCCTGGGCTATCTCACCC ACCCTGGCTTACCATAGCCTCCTTAAACCACAGGGGTCTGGTGGCCTGATATGCATCGGCTGGCTGACCG TATCCACCTGGAGGAACTGGCACGCATTGGCCTCCTGAGTGGCAGTGTGGATGCCATGCTCCAGGTCCAC CTGGGAGCAGTATTCATGCCACATGGACTTGGCCACTTTCTGGGCCTAGATGTGCACGACGTGGGAGGCT ACCCTGAGGGTGTAGAGCGCATCGATGAACCTGGCCTGCGAAGCCTGCGTACGGCAAGGCACCTGGAGCC GGGCATGGTGCTGACTGTGGAGCCAGGCATCTACTTCATTGACCACCTCTTGGACCAGGCCCTGGCAGAC CCGGCCCAAGCCTGCTTCTTTAACCAAGAGGTCCTGCAGCGCTTCCGCAACTTCGGTGGGGTGCGGATCG AGGAAGATGTTGTCGTGACTGACAGCGGCATGGAGCTGCTGACATG
Notes:	The probe template was PCR amplified from IMAGE:1513277 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1513277 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14735 same embryo
	EMAGE:14732 same embryo
	EMAGE:14734 same embryo
	EurExpress:euxassay_005690 same experiment
	MGI:4827155 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source