EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14760

Tspo translocator protein ( MGI:88222)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005714. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005714_01	euxassay_005714_02	euxassay_005714_03	euxassay_005714_04

euxassay_005714_05	euxassay_005714_06	euxassay_005714_07	euxassay_005714_08	euxassay_005714_09

euxassay_005714_10	euxassay_005714_11	euxassay_005714_12	euxassay_005714_13	euxassay_005714_14

euxassay_005714_15	euxassay_005714_16	euxassay_005714_17	euxassay_005714_18	euxassay_005714_19

euxassay_005714_20	euxassay_005714_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14760_wholemount_strong.wlz

14760_wholemount_moderate.wlz

14760_wholemount_weak.wlz

14760_wholemount_possible.wlz

14760_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14760_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver

moderate

regional

moderate expression: see section 01 02 04 05 06 07 10 11 14 17 18 21 weak expression: see section 03 08 09 12 13 15 16 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6101
Entity Detected:	Tspo, translocator protein ( MGI:88222)
Sequence:	sense strand is shown >T6101 GGAACAACCAGCGACTGCGTGAGCGGGGCTGTGGATCTTTCCAGAACATCAGTTGCAATCACCATGCCTG AATCCTGGGTGCCTGCCGTGGGCCTCACTCTGGTGCCCAGCCTGGGGGGCTTCATGGGAGCCTACTTTGT ACGTGGCGAGGGCCTCCGGTGGTATGCTGGCTTGCAGAAACCCTCTTGGCATCCGCCTCGCTGGACACTG GCTCCCATCTGGGGCACACTGTATTCAGCCATGGGGTATGGCTCCTACATAGTCTGGAAAGAGCTGGGAG GTTTCACAGAGGACGCTATGGTTCCCTTGGGTCTCTACACTGGTCAGCTGGCTCTGAACTGGGCGTGGCC CCCCATCTTCTTTGGTGCCCGGCAGATGGGCTGGGCCTTGGCCGATCTTCTGCTTGTCAGTGGGGTGGCG ACTGCCACAACCCTGGCTTGGCACCGAGTGAGCCCGCCGGCTGCCCGCTTGCTGTACCCTTACCTGGCCT GGCTGGCTTTTGCCACCGTGCTCAACTACTATGTATGGCGTGATAACTCTGGCCGGCGAGGGGGCTCCCG GCTCGCAGAGTGAAGGCACCCAGCCATCAGGAATGCAGCCCTGCCAGCCAGGCACCATGGGTGGCAGCCA TCATGCTTTCATGACCATTGGGCCTGCTGGTCTACCTGGTCTTAGCCCAGGAAGCCACCAGGTAGGTTAG GGTGGTCAGTGCCGAGTCTCCTGCAGACACAGTTATACCTGCCTTTCTGCACTGCTCCAGGCATGCCCTT AGAGCATGGTGTTTTAAAGCTAAATAAAGTCTCTAACTTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3493196 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3493196 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14763 same embryo
	EMAGE:14762 same embryo
	EMAGE:14759 same embryo
	EMAGE:14761 same embryo
	EMAGE:14764 same embryo
	EurExpress:euxassay_005714 same experiment
	MGI:4828972 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source