EMAGE mouse gene expression spatial database

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EMAGE:14763

Ppid peptidylprolyl isomerase D (cyclophilin D) ( MGI:1914988)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005718. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005718_01	euxassay_005718_02	euxassay_005718_03	euxassay_005718_04

euxassay_005718_05	euxassay_005718_06	euxassay_005718_07	euxassay_005718_08	euxassay_005718_09

euxassay_005718_10	euxassay_005718_11	euxassay_005718_12	euxassay_005718_13	euxassay_005718_14

euxassay_005718_15	euxassay_005718_16	euxassay_005718_17	euxassay_005718_18	euxassay_005718_19

euxassay_005718_20	euxassay_005718_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14763_wholemount_strong.wlz

14763_wholemount_moderate.wlz

14763_wholemount_weak.wlz

14763_wholemount_possible.wlz

14763_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14763_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7181
Entity Detected:	Ppid, peptidylprolyl isomerase D (cyclophilin D) ( MGI:1914988)
Sequence:	sense strand is shown >T7181 GTTATAACGTAGAGGGGCAGTAGAGTTTGCTCCGGTCTCGCGCACGAGGTGCTCGTGTCCCGCTGCCCAG TGCCCGCAGTGAAGATGTCCCACGCATCCCCGGCAGCCAAGCCCTCCAACTCCAAGAACCCGCGAGTCTT CTTTGACGTGGACATCGGCGGGGAGCGAGTTGGCCGAATTGTTTTAGAATTGTTTGCAGATATTGTTCCC AAAACTGCAGAAAATTTTCGTGCGTTGTGTACAGGAGAAAAAGGAACTGGGTCCACGACCGGGAAGCCTC TCCATTTTAAAGGATGCCCTTTCCACCGAATTATTAAGAAATTTATGATTCAGGGTGGAGACTTCTCAAA TCAGAATGGGACAGGTGGCGAAAGTATTTATGGTGAAAAATTTGAAGATGAAAATTTTCATTATAAGCAT GATCGGGAGGGTTTGCTGAGCATGGCAAATGCAGGCCCCAATACGAATGGCTCTCAGTTCTTTATCACAA CAGTTCCGACTCCTCATCTGGACGGGAAACATGTGGTATTTGGTCAGGTCATAAAAGGACTAGGTGTGGC AAGGACGCTTGAAAATGTAGAGGTGAATGGTGAAAAACCTGCCAAACTCTGTGTTATTGCAGAATGTGGA GAATTGAAAGAAGGGGATGACTGGGGAATCTTCCCCAAAGATGGCTCTGGTGACAGTCATCCAGACTTCC CTGAGGATGCAGATATAGATTTAAAAGATGTAGATAAAATTTTATTAATATCTGAAGACTTAAAAAACAT TGGAAATACTTTTTTCAAGTCTCAGAACTGGGAGATGGCTATTAAAAAATATGCGAAGGTTTTAAGGTAT GTGGATAGTTCAAAGGCTGTTATTGAGAAAGCAGATAGATCCAGACTGCAACCTATAGCCTTAAGTTGTG TGCTGAATATTGGTGCTTGTAAGCTGAAGATGTCAAATTGGCAGGGGGCAATTGACAGTTGCTTAGAGGC TCTTGAAATGGACCCTTCAAATACTAAAGCACTATACCGAAAAGCGCAAGGATGGCAAGGATTGAAAGAA TATGATCAAGCATTGGCTGATCTTAAGAAGGCACAGGAGATAGCCCCAGGAGATAAAGCTATCCAGGCAG AATTGCTTAAAGTCAAACAAATGATAAAGGCACAGAAAGATAAAGAGAAGGCCGTGTATGCAAAAATGTT TGCTTAATAATGATTAAACTCTCTTTAACTATTATACATTGATTATCTAAAGGACAAGGTATATTACTGT CTACATGCTCCCTGTTGTGTTTCCCTTGATCTCTGTCATTGTTTACATAGCAGTGCTGACACAGGTTCCT TCTTAATAAAGAGTTAAAAAATACAGTGAATCTCATTTTAAAATGTTTGTCTGCATTTTCACCAAGAGTG TATCTGGCCAATGTTTAAACTCTAACTCTCACTAAATTTTCGGCTTTGTGGATGGTCTAGCCCAGCAGTT CTTAACCTGATGGTCCACTGAAAAACATAGATATTTTTACAGTATGGTTCAGAGCAGTAGCAAAATTACA GTTGTGAAGTAGCAACAAAAATAAATTTATGTTTGGGGGTCACCACATGAAGAACTGTACTAAAAGGTTG CAGCACTAGTAAGGTTGGGAACCACTGATGTAACTGTCACGTTTATGTGTATTAACCAGCTATGTGTATA AACCATCGGGCATGGCTGTGTTTCAATAAATCGGTTTTTAAGTGTTCAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4035785 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4035785 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14760 same embryo
	EMAGE:14762 same embryo
	EMAGE:14759 same embryo
	EMAGE:14761 same embryo
	EMAGE:14764 same embryo
	EurExpress:euxassay_005718 same experiment
	MGI:4827357 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source