EMAGE mouse gene expression spatial database

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EMAGE:14788

Pcna proliferating cell nuclear antigen ( MGI:97503)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005732. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005732_01	euxassay_005732_02	euxassay_005732_03	euxassay_005732_04

euxassay_005732_05	euxassay_005732_06	euxassay_005732_07	euxassay_005732_08	euxassay_005732_09

euxassay_005732_10	euxassay_005732_11	euxassay_005732_12	euxassay_005732_13	euxassay_005732_14

euxassay_005732_15	euxassay_005732_16	euxassay_005732_17	euxassay_005732_18	euxassay_005732_19

euxassay_005732_20	euxassay_005732_21	euxassay_005732_22	euxassay_005732_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14788_wholemount_strong.wlz

14788_wholemount_moderate.wlz

14788_wholemount_weak.wlz

14788_wholemount_possible.wlz

14788_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14788_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 13

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 19 20 21 22 weak expression: see section 17 18

telencephalon mantle layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 19 20 21 22 23 weak expression: see section 17 18

telencephalon ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 19 20 21 22 weak expression: see section 17 18

hindbrain

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 12

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 12

pons ventricular layer

strong

regional

strong expression: see section 12

midbrain

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain ventricular layer

strong

regional

strong expression: see section 12 13

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3871
Entity Detected:	Pcna, proliferating cell nuclear antigen ( MGI:97503)
Sequence:	sense strand is shown >T3871 AATTCGACCGAGGAAGCCGCGGCATTAGACGGTTGCGGGCGCAGAGGGTTGGTAGTTGTCGCTGTAGGCC TTCGCTGCCGCTTCTGCATCGTGAATCGGGGGACCTTGGCAGCAAGACCTCGTTTCTCTTAGAGTAGCTC TCATCTAGTCGCCACAACTCCGCCACCATGTTTGAGGCACGCCTGATCCAGGGCTCCATCCTGAAGAAGG TGCTGGAGGCTCTCAAAGACCTCATCAATGAGGCCTGCTGGGACGTCAGCTCGGGCGGCGTGAACCTGCA GAGCATGGACTCGTCTCACGTCTCCTTGGTACAGCTTACTCTGCGCTCCGAAGGCTTCGACACATACCGC TGCGACCGCAACCTAGCCATGGGCGTGAACCTCACCAGCATGTCCAAAATTCTAAAATGTGCTGGTAATG AAGACATCATTACATTAAGGGCTGAAGATAATGCAGACACCTTAGCACTAGTATTCGAAGCACCAAATCA AGAGAAAGTTTCAGACTATGAAATGAAGTT
Notes:	The probe template was PCR amplified from IMAGE:1382395 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1382395 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14790 same embryo
	EMAGE:14789 same embryo
	EMAGE:14792 same embryo
	EMAGE:14791 same embryo
	EurExpress:euxassay_005732 same experiment
	MGI:4827097 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source