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EMAGE:14796

Prdm4 PR domain containing 4 ( MGI:1920093)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14796 EMAGE:14796 EMAGE:14796 EMAGE:14796 EMAGE:14796
"Pseudo-wholemount" of euxassay_005781. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005781_01 euxassay_005781_02 euxassay_005781_03 euxassay_005781_04
EMAGE:14796 EMAGE:14796 EMAGE:14796 EMAGE:14796 EMAGE:14796
euxassay_005781_05 euxassay_005781_06 euxassay_005781_07 euxassay_005781_08 euxassay_005781_09
EMAGE:14796 EMAGE:14796 EMAGE:14796 EMAGE:14796 EMAGE:14796
euxassay_005781_10 euxassay_005781_11 euxassay_005781_12 euxassay_005781_13 euxassay_005781_14
EMAGE:14796 EMAGE:14796 EMAGE:14796 EMAGE:14796 EMAGE:14796
euxassay_005781_15 euxassay_005781_16 euxassay_005781_17 euxassay_005781_18 euxassay_005781_19
EMAGE:14796 EMAGE:14796 EMAGE:14796 EMAGE:14796
euxassay_005781_20 euxassay_005781_21 euxassay_005781_22 euxassay_005781_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14796Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14796_wholemount_strong.wlz
14796_wholemount_moderate.wlz
14796_wholemount_weak.wlz
14796_wholemount_possible.wlz
14796_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14796_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35521
Entity Detected:Prdm4, PR domain containing 4 ( MGI:1920093)
Sequence:sense strand is shown

>T35521
CCATATCCACAATCATCTCCCTAGCCAGGGCCACAGCAGCAGCCATGGGCCAAGCCACAGCAAGGAAAGG
AAGTGGAAGTGTTCAATGTGCCCCCAGGCTTTTATCTCGCCTTCCAAACTCCACGTTCACTTTATGGGCC
ACATGGGTATGAAGCCCCACAAGTGTGATTTTTGTAGCAAGGCTTTTAGTGATCCAAGCAACCTACGGAC
ACACCTCAAAATACATACAGGTCAGAAAAATTATAGGTGTACTTTGTGTGACAAGTCTTTCACGCAGAAG
GCTCACCTGGAGTCTCACATGGTCATCCACACGGGTGAGAAGAATCTCAAGTGTGATTACTGTGACAAGC
TGTTCATGCGGAGACAGGACCTCAAGCAGCACGTGCTCATCCACACGCAAGAACGCCAGATCAAGTGTCC
GAAGTGTGATAAACTGTTCTTGAGAACAAACCACTTGAAGAAGCATCTCAATTCTCACGAAGGAAAACGA
GATTATGTCTGCGAAAAATGTACAAAGGCTTATCTAACCAAGTACCATCTCACTCGCCACCTGAAGACCT
GCAAAGAGCCCAGCTCCAGCTCCTCTGCACAGGAGGAAGAGGATGATGAGTCGGAGGAAGAAGATCTCGC
AGACTCCATGAGGACAGAGGACTGCAGGATGGGCAGTGCTGTTTACTCAACAGACGAGTCTCTCTCCGCA
CATAAATAGGAGGAGAGAGCAACTGCTTTGGACGGAACACACATTGGTAAAACAAAACCAACCCACGGTT
AGAAGTGGACGGAGTGGGGACAGCTAAAGCACTTACAGAGGAATGTCCAGTGGATTCTTTAAGATGGACT
GGCAGATGAGGCATGCATCTGTCCTACTTTTCAGTAATGGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 82771. Forward Primer - name:082771_F_cDNA_Prdm4, sequence:CCATATCCACAATCATCTCCCT; Reverse Primer - name:082771_N_SP6_cDNA_Prdm4, sequence:CCCATTACTGAAAAGTAGGACAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14794 same embryo
 EMAGE:14797 same embryo
 EMAGE:14798 same embryo
 EMAGE:14793 same embryo
 EMAGE:14795 same embryo
 EurExpress:euxassay_005781 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS