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EMAGE:14821

Rhobtb1 Rho-related BTB domain containing 1 ( MGI:1916538)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14821 EMAGE:14821 EMAGE:14821 EMAGE:14821 EMAGE:14821
"Pseudo-wholemount" of euxassay_005799. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005799_01 euxassay_005799_02 euxassay_005799_03 euxassay_005799_04
EMAGE:14821 EMAGE:14821 EMAGE:14821 EMAGE:14821 EMAGE:14821
euxassay_005799_05 euxassay_005799_06 euxassay_005799_07 euxassay_005799_08 euxassay_005799_09
EMAGE:14821 EMAGE:14821 EMAGE:14821 EMAGE:14821 EMAGE:14821
euxassay_005799_10 euxassay_005799_11 euxassay_005799_12 euxassay_005799_13 euxassay_005799_14
EMAGE:14821 EMAGE:14821 EMAGE:14821 EMAGE:14821 EMAGE:14821
euxassay_005799_15 euxassay_005799_16 euxassay_005799_17 euxassay_005799_18 euxassay_005799_19
EMAGE:14821 EMAGE:14821 EMAGE:14821 EMAGE:14821
euxassay_005799_20 euxassay_005799_21 euxassay_005799_22 euxassay_005799_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14821Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14821_wholemount_strong.wlz
14821_wholemount_moderate.wlz
14821_wholemount_weak.wlz
14821_wholemount_possible.wlz
14821_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14821_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35529
Entity Detected:Rhobtb1, Rho-related BTB domain containing 1 ( MGI:1916538)
Sequence:sense strand is shown

>T35529
TTAGCAAGGCCCATAAAGAGAGGTGACATTTTGCCCCCAGAAAAAGGCCGCGAGGTGGCGAAGGAACTTG
GCATCCCGTACTACGAGACCAGCGTGTTTGACCAGTTTGGCATCAAGGACGTGTTCGACAATGCCATCCG
AGCAGCCCTCATCTCCCGCCGGCACCTGCAGTTCTGGAAATCCCACTTGAAAAAAGTCCAGAAGCCTCTG
CTTCAGGCGCCCTTCCTACCTCCGAAAGCTCCTCCCCCGGTCATCAAGGTCCCCGAGTGTCCGTCTGCGG
GGACCAGCGATGCTGCCTGTTTACTGGACAACCCCCTGTGCGCCGACGTCCTCTTCGTTCTCCATGACGA
GGAGCACATCTTTGCGCACCGCATTTACCTCGCTACCTCTTCTTCCAAATTCTACGACCTTTTTTTAATG
GAGTGTGAAGAATCCCCTTGTTGGGGTGGAGGAGCTGGTGAGGAGGTGCCGTGCAGGGATTTTCAGGGGC
GGACGCAGAGTCTTGGATCAGCCGAGGAAGGGAAGGAGGGCCCCCAGAGGACCCCTCAGGCTGACCCAGG
AGCGTCTTCAGGCCAGGACCTGCCTGAGAGTTTGGCTTTGCAGATGGAAGCCTCGGGTTCCGAGGGACAC
GCCCTGTCCGGCTGGAGCAAGGGGTTTGTCAGCATGCACAGGGAGGTGCGGGTCAACCCCATTTCCAAGC
GGGTGGGCCCAGTGACCGTGGTCAGGCTGGACCCATCGATGCAGTCAGGCCCTTTCCGGACCCTGCTGAG
ATTCCTCTACTCTGGGCAGCTGGACGAAAAGGAAAAAGATCTGCTGGGGCTGGCTCAGATGGCCGAGGTC
CTGGAGATGTTTGATCTGAGGATGATGGTAGAGAATATCATGAACAAGGAGGCTTTTATGAACCAGGAGA
TCACGAAAGCCTTCCACGTCCGGAAAGCCAATCGGATCAAAGAATGTCTCAGCAAGGGCAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 82676. Forward Primer - name:082676_F_cDNA_Rhobtb1, sequence:TTAGCAAGGCCCATAAAGAGAG; Reverse Primer - name:082676_N_SP6_cDNA_Rhobtb1, sequence:GTGCCCTTGCTGAGACATTCTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14825 same embryo
 EMAGE:14820 same embryo
 EMAGE:14823 same embryo
 EMAGE:14822 same embryo
 EMAGE:14824 same embryo
 EurExpress:euxassay_005799 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS