EMAGE mouse gene expression spatial database

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EMAGE:14826

Stk32c serine/threonine kinase 32C ( MGI:2385336)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007944. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007944_01	euxassay_007944_02	euxassay_007944_03	euxassay_007944_04

euxassay_007944_05	euxassay_007944_06	euxassay_007944_07	euxassay_007944_08	euxassay_007944_09

euxassay_007944_10	euxassay_007944_11	euxassay_007944_12	euxassay_007944_13	euxassay_007944_14

euxassay_007944_15	euxassay_007944_16	euxassay_007944_17	euxassay_007944_18	euxassay_007944_19

euxassay_007944_20	euxassay_007944_21	euxassay_007944_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14826_wholemount_strong.wlz

14826_wholemount_moderate.wlz

14826_wholemount_weak.wlz

14826_wholemount_possible.wlz

14826_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14826_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4132
Entity Detected:	Stk32c, serine/threonine kinase 32C ( MGI:2385336)
Sequence:	sense strand is shown >T4132 GACCCACGCGTCCGAGGAGAACGGCCGGCGTTGCCGGCCCTTAGCGCTCTGCTCTCCCAGGGTCGGGCGC GGGCCATGTTCCGGGCTGTGGCCGCTGCGCATACCCAGGCCTGGCGATGAGGAGCGGGGCGGAGAGAAGG GGCAGCAGCGCCGCGGCGCCCCCCAGTTCGCCGCCTCCCGGTCGTGCGCGCCCCGCGGGCTCCGATGTGT CCCCCGCCTTGCCGCCGCCGGCAGCTAGCCAGCCCCGGGCCCGGGACGCCGGAGATGCCCGCGCGCAGCC GCGCCCCCTGTTTCAGTGGAGCAAGTGGAAGAAGAGGATGTCTATGTCGTCCATATCGTCAGGCAGCGCG CGGAGGCCGGTGTTTGACGACAAGGAGGACGTGAACTTTGACCACTTCCAGATTCTGAGAGCAATTGGGA AAGGCAGCTTTGGCAAGGTGTGCATCGTGCAGAAGCGGGACACAGAGAAGATGTACGCCATGAAGTACAT GAACAAGCAGCAATGCATAGAGCGGGATGAGGTCCGGAATGTCTTCCGGGAGCTGGAGATCCTACAGGAA ATCGAGCATGTCTTCCTGGTGAACCTCTGGTACTCCTTCCAGGATGAGGAGGACATGTTCATGGTGGTGG ATCTGCTTCTGGGGGGAGACCTGCGCTACCACCTACAGCAGAATGTCCAGTTCTCAGAGGACACAGTGAG GCTGTACATCTGTGAGATGGCCCTGGCCCTGGACTACCTGCGTAGCCAACACATCATCCACAGAGATGTC AAGCCTGACAACATCCTCCTGGATGAACAAGGACATGCACACCTTACTGACTTCAACATTGCCACCATCA TAAAGGATGGGGAGAGGGCGACAGCTTTAGCTGGGACCAAACCGTACATGGCTCCAGAGATCTTCCACTC TTTCGTCAATGGCGGGACTGGCTACTCCTTCGAGGTGGACTGGTGGTCGGTGGGGGTGATGGCTTACGAG CTGCTACGAGGATGGAGACCCTACGATATCCACTCGAGCAATGCTGTGGAGTCGCTGGTTCAGCTGTTCA GCACTGTGAGTGTCCAGTACGTGCCCACCTGGTCCAAGGAGATGGTAGCCCTGCTGCGAAAGCTCCTCAC CGTGAACCCTGAGCACCGGTTCTCCAGTCTCCAAGACATGCAGACAGCCCCATCGCTGGCCCACGTGCTG TGGGACGACCTGAGTGAAAAGAAGGTGGAGCCAGGCTTTGTGCCCAATAAAGGCCGCCTGCACTGTGATC CCACCTTTGAGCTGGAGGAGATGATCCTTGAGTCGAGGCCTCTGCACAAGAAGAAAAAGCGGCTGGCCAA GAATAAGTCTCGGGACAGCAGCAGAGACAGTTCCCAGTCGGAGAATGACTACCTGCAAGACTGCCTGGAT GCCATCCAGCAAGACTTCGTGATTTTTAACAGAGAAAAGTTGAAGAGGAGCCAGGAACTCATGAGCGAGC CTCCTCCAGGCCCAGAGACGTCAGATATGACAGATTCCACCGCAGACAGTGAGGCAGAGCCCACAGCCCT GCCCATGTGTGGCTCCATCTGCCCCTCATCTGGGAGCAGCTAGGCCATCTGGACCGTGTCCGAGGGCAGT TCTCTCTCAGGCTGCTTTGAAGACTCGGGACGGCCAGAAGGAGGCAGCAGTGGGGCAAAGGCCTGACCGC CCAGCCTGCCCAGGGAGGCTGTCTGTACGGTGAAGGTGCTGGGAGCCATGGGTGCCCGTCAAGAGTGGTG GTTGGGCAAGGAGGGCCCTTGGTACCTAGACCATGGTTCCTTGCAACATAGAGGGAGTCATGCGGCCACC ATGCTGGCTGGCCCCTTACTGTAGGTTGGCAGATTATAGGTGGCCACACTGGCACAGTGGGGCAGGAGCC CTTGGCTTTCTGGACCCTTTTGGAATTGGCTGTACATAGCCTTGTGGCCAGTCTTCGCTGTGGCCTAGTC AGAATGTTCTTGAGCCTGCTGCTCAGGGCCGTGGGGGCGGGAACAGGCCTTTCTGATCCTGATATGTTAT TGAGGGTAAGGGTTGGGGGAGGGAGTGGGTGGGAGGGCAAAGAACGTTTTCTAGTGACACACGCTGACTG ACTGCTCTGGCAATTTCTGCAACTTGTGTATAGTTTTCACCTCTGAGAATTACAGTCTAAGAACTGCAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4481218 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4481218 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14827 same embryo
	EMAGE:14830 same embryo
	EMAGE:14831 same embryo
	EMAGE:14828 same embryo
	EMAGE:14829 same embryo
	EurExpress:euxassay_007944 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source