EMAGE mouse gene expression spatial database

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EMAGE:14829

Etv3 ets variant gene 3 ( MGI:1350926)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007938. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007938_01	euxassay_007938_02	euxassay_007938_03	euxassay_007938_04

euxassay_007938_05	euxassay_007938_06	euxassay_007938_07	euxassay_007938_08	euxassay_007938_09

euxassay_007938_10	euxassay_007938_11	euxassay_007938_12	euxassay_007938_13	euxassay_007938_14

euxassay_007938_15	euxassay_007938_16	euxassay_007938_17	euxassay_007938_18	euxassay_007938_19

euxassay_007938_20	euxassay_007938_21	euxassay_007938_22	euxassay_007938_23	euxassay_007938_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14829_wholemount_strong.wlz

14829_wholemount_moderate.wlz

14829_wholemount_weak.wlz

14829_wholemount_possible.wlz

14829_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14829_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4395
Entity Detected:	Etv3, ets variant gene 3 ( MGI:1350926)
Sequence:	sense strand is shown >T4395 GACGGACGGCGCCGGAGAGGAGGGCGAAAATGAAAGCAGGCTGTAGCATCGTGGAAAAGCCAGAAGGAGG TGGAGGGTATCAGTTTCCGGATTGGGCCTACAAAGCCGAGTCGTCGCCGGGCTCCCGGCAGATCCAGCTG TGGCACTTCATCCTGGAACTGCTGCAGAAGGAAGAGTTCCGCCATGTCATCGCCTGGCAGCAGGGAGAGT ACGGGGAGTTTGTCATCAAGGATCCAGATGAAGTGGCTCGCCTCTGGGGCCGCAGGAAGTGCAAACCACA GATGAACTATGACAAGCTGAGCCGGGCCCTCAGATACTATTACAACAAGAGGATCCTTCATAAAACAAAA GGGAAAAGGTTTACTTACAAGTTTAACTTCAACAAGCTCGTGATGCCCAACTACCCGTTCATCAACATTC GCTCGAGTGGTGTGGTTCCTCAGAGTGCGCCACCAGTGCCAACAGCCTCCTCCCGGTTCCATTTCCCACC TCTAGACAGCCATTCTCCTACTGGCGATGTGCAACCGGGTCGCTTCTCTGCCAGCTCACTGAGTGCTTCT GGCCCTGAGTCAGGGGTTACCACTGACAGGAAGGTCGAGCCTTCGGACCTGGAAGATGGCTCGGCCTCTG ACTGGCACCGGGGCATGGACTTCATGCCCTCCCGAAATGCTCTGGGCGGAGGAGCAGTCGGCCACCAGAA ACGCAAGCCTGACATACTGCTTCCTCTCTTCACCCGGCCAGCCATGTACCCTGACCCGCACAGTCCCTTC GCTATCTCTCCGGTCCCTGGCCGCGGAGGGGTCCTTAATGTCCCCATCTCACCAGCCCTGTCCCTGACTC CCACCATGTTCTCCTACAGTCCCTCACCAGGCCTGAGCCCCTTCACCAGCAGCAGTTGCTTCTCCTTCAA CCCAGAGGAAATGAAACACTACCTTCATTCTCAAGCCTGTTCGGTGTTCAACTACCATCTGAGTCCCCGG ACTTTCCCCCGTTACCCAGGGCTCATGGTCCCACCGCTGCAGTGCCAAATGCATCCTGAGGAGCCTTCCC AGTTCTCTATCAAGCTGCAGCCCCCGCCGGCTGGACGGAAGAACCGCGAGAGGGTAGAGAGCCGGGAGGA GGCTGTTCGCGGCTCTGTGCCCGCCAGCGCTCCTGTTCCCTCTCGGATTAAGGTAGAGCCGGCCACAGAG AAGGATCCTGACAGCCTCCGGCAGTCAACCCAGGGAAAGGAGGAACAGACCCAAGAAGTGGACAGCATTC GGAGCAGGACCATAGAAGAGGGGAAAGGCACCGGGTTTGCCCACCCCTCACCCACCTGGCCCTCTGTGTC CATTAGCACTCCCAGTGACGAACCCCTAGAGGGGACCGAAGACAGTGAGGACAGGTCTGCCAGGGAGCCC GGTGTACCCGAGAAGAAAGAAGACGCCCTGATGCCCCCTAAGCTTCGGCTGAAGAGGCGGTGGAATGACG ACCCTGAAGCCAGGGAGCTCAACAAAACGGGCAAGTTCCTCTGGAATGGGGCAGGACCCCAGGGCCTGGC CACAACGGCCACTGCTGCCGCTGATGCTTAAAACCACAGTGGAAGGGAAGCTGTTCATATTACAATCAAA ATACATATTTATGTAGCAAGATTCTGGGGCGGGAGGTGGGGTTCAGCTGGTTTGATAATTCTGGGGCGTG GACTTGTACTTTTCTGTTGGGGATGGGACGAGGATCTTGTGTTGTATAAATAAGTGGGGTATGGACACTT TTTTTTCTTGGCGCGTAGGACATAGGGAGGCTATGAACTTTGGGGAAGGAGCCTGTTTCCTGTCCCCTCC TGCTGCCTGACAAGCTCCATAGGCCCACACACTGTAGTCCCATCACAGGTCAGGCCCTATAGTCTTTCCT ATTGTGTTTATACATACAGAAAGCCCATAGTGGGATAGTTTGCTTTGAGAGGGAGAGAGAAGAGGAAGAG GCTGGAAGGTTAGGCGTGAGAAACATACTAACCATGTACCTGAGTATGTGTTTCAGGAGAGGAAAAAGTT CATCGCAGTCTTACCGAAGTGGGGGGCATTTAGATGGGGTTCTCTACTCCCCTCTCCCCCTTCTCCCTCA CTGGAGTGTGTGTGTGTGTGATGTGTGTGTCTATGTGTGTGTGCGCACACACACAGAGACACACATATGC TCTCTCCCATGCATGCACTCTCTCTTTCTCTCCCTGGGGGGAGGGTGACACACGCACATTTGGCTCCCCT CCCCCCTTTTTAGAAAATGTTTTCAAGACACATGAGTGTACTGCCAGTTATACTGGGCAGGGAGGGCACC GGAGACCCCCCACCCCCGGCCCCTCAGCTTCAGAGTTGAGGTGGGAAGGCAGCCTGGCTGCGAGGTCCTC GAACTCTCACCTTCTTCACCTTCAAAAAGCCATGAGGAGTTGTAAACTCTTGATCAGGGAAGAAAGAAGA GGGCAGGAGGAAGTAACCCAATGCCTTTAAAAACAAAACAAATGACAGAAATAGTCTTCATTTTCTTTTT CTTTTTCCATTGTTGGTTTGGTTTCCCAAACCAAGCGTGACTGAGCACGTTTTATGAGGAGCAGGATAGC GCCGTTGAAGATGTTTTTCTGTTGTGTCTGGTTGTCTTACTGTTTTCTTAAGTCGAGCCTTAACTACGAA CATGCTTTAAGATGATACGGGTCTGTCAACATAAACCTGTGAAGGGCATGGACGCTGTTCAGATAACCCA GGAATGTTGAGGCACAGTCGAATTCCTAGCTGATAGTTATTCCCTCTGATGTTGCCGGTCCGGGTGGCAA AAGGGCAGAATTGTGGGAATGAAAGGCAGATGATAACCTGCAAACACAATGTACTGCTTGTCGAAACTGA AGGAAGAGGTAGGAAGGTGGTGACCAGATGCCAAAAGGTCTGGTGTAAAGGAGAGAGCCGGTGTTGCCTG CGGTGGTGTTGCCCGCCTTGTGGTCCCCCGTTGCCCAGATTGTTCTGATCGGTCGACTGTTCCCAAAATG CAGTTCACCAACAGGGGTCGTTTGTCCTTGGGAAATCCCCTTACAGCAACTTCAAAGGAATGGGAAGATA CTGATCTTAGGTGAAACAAAACACTAGCACTAGGGTAGAGAGAAAGCGGCACCAGCCAACGGCCTCTGTG CACGTGTGTGCTCTAGTAGCTCAGAGCAGCAAGAAGAAATGGTTTCCTATCTGCTGAGGCAAGGGATGGC ACTTGGGCATCAGAGTGATGACTCACTCCGTCCGCCACCTGAGCCTGGCCAAGAACTCCATACTAAGTGA CATTTGAGTGTTGTCTTCTGATGGAGTGTTTGGGTTTGGTTTAGTTTGGTTTTATTTTCTTTAACTGAAA TGCATGCACAAAAAGAGCTCAGGAGACATTAGACCTGGTGAAAGGTGTGAGCTGACGTGGATCACTTGAG GCTGTCATTGCTGTTTCTTGATGAGTGTCCTATAACTTGGTCCAGATTCCACGCATGTGTGAAAGGCCAG GAAGGGGGCCGAGTGGTCTCTGGTGACCCAGGCTCTGGTCAGGCCACGAAGGACTTCAACCCTCCTGTTG CCTTTGGTGGTTGTGTTTTGATCAGTTTCCTTCTCTGCACCTTGACGGACCTCTGAATCAGAAAGCAGGC CCAGCAGGTAGAGGAAGGCTCTCTGGTTGGTGTTGCCCATCAAACCAGGGAGGCCAGCTGGCCACCGGTT GTCTGCTAGTGGGGACGACCAGCGAGCATCAGTATGAACTCAGGAATAGAAGCGTGGGGCCTTTAAAATG AGGGCGGGAAATCCATGATGCATACCTGAAACCCCTAGAACCCAAGTGCCGGAATTCATTCTAGATGCTG CTTCCATTTGAATAAAGCCACTGCTTTTACACTTGAAAGTGAACTCCATGAAAAATGCTTTTGGACTTTT ATGAAACTATTTGCCGTTTGTTTCCTTTGATTGCCATTCCACCAGTAATCTCTTGGTTGATTGGCACTGC ACACAGTTGGAGTCTGGATCTGGGGAAGGGTCTGTCTGCAGAGCAGAACTTGGGCTCGCTCAGGAAGTGG ACCAGGAAATGTAGTGATCACTGATACTTCCTGGGGCAGAGGAGTGAGAGTGCTCGTCTCTCCCCAGCCC CAGCCTGGCAGTTCCTGCCTCCAGCCTGGTGTCACCTTCTTTCTACGAGCACGCCATTGACCATCAGCCA GGCGAAAGGGGGCTAAAAGCCGGGCCTACTTCCTCTCCCTGGGTTTTTTCTTCCCTCTAAATTTTGTTGT GCCAAATGTGTAAAACTTTGTAGGTGTAAATCTATTGCAATCTCACTAATCAAGAGCTTGTTTATTTAAA CAGTTTGCTTTTTTAGCTGTAAAGAATAGCAGACTCTCGGGGCATCAGCTCTGCTGTGCTCGCGGGTCTT CCTAGACATCAGGCTGCACTTCTAACCCCAGCTCGGGAATGCAGGGAACCCTAGTGACCCATAGGCCAAG AAACTTCCAGAAGCATTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4918332 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4918332 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14827 same embryo
	EMAGE:14830 same embryo
	EMAGE:14831 same embryo
	EMAGE:14828 same embryo
	EMAGE:14826 same embryo
	EurExpress:euxassay_007938 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source