EMAGE mouse gene expression spatial database

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EMAGE:14830

Pkn3 protein kinase N3 ( MGI:2388285)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005796. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005796_01	euxassay_005796_02	euxassay_005796_03	euxassay_005796_04

euxassay_005796_05	euxassay_005796_06	euxassay_005796_07	euxassay_005796_08	euxassay_005796_09

euxassay_005796_10	euxassay_005796_11	euxassay_005796_12	euxassay_005796_13	euxassay_005796_14

euxassay_005796_15	euxassay_005796_16	euxassay_005796_17	euxassay_005796_18	euxassay_005796_19

euxassay_005796_20	euxassay_005796_21	euxassay_005796_22	euxassay_005796_23	euxassay_005796_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14830_wholemount_strong.wlz

14830_wholemount_moderate.wlz

14830_wholemount_weak.wlz

14830_wholemount_possible.wlz

14830_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14830_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

heart ventricle

strong

spotted

strong expression: see section 12 13 14 15 16 moderate expression: see section 07 08 09 weak expression: see section 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4377
Entity Detected:	Pkn3, protein kinase N3 ( MGI:2388285)
Sequence:	sense strand is shown >T4377 GGCTTAGGAGATGGAACACCGAAAGCCTGGGACTGGCCAGAGAGCCCCCAAGGATGAGAAAGAGATGGTC CGCCGAGCCATCCAGAAGGAGCTGAAGATCAAGGAGGGCATGGAGAACATGCGGCGCGTGGCCACAGACC GACGCCACCTGGGTCATGTGCAGCAACTGCTTAGGGCCTCCAACCGCCGCCTGGAGCAGCTGCATGGCGA GCTTCGGGAGCTGCATGCCCAAGTTCTGCTGCCAGCCTCAGCCGAGCCAGTGACCTCGGAACCCCAGCCG CGGGCGGAGCAGTCCAGGGCTCGGCTCTCGGAGGCTTTACATAGGCAGCTGCAGGTAGAGCTAAAGGTAA AGCAGGGGGCTGAGAATATGATTCACACGTGTGCCAGCGGCACCCCCAAGGAGAGGAAGCTCCTGGCGGC TGCCCAGCAGATGTTAAAGGACAGTCAACTGAAGGTGGCCCTGCTCCGGATGAAGATAAGCAGCCTGGAG TCCAGTGGCTCTCCAGAGCCAGGCCCCGACTTGCTGGCAGAGGAGCTGCAGCACCGACTTCGAGTAGAGG CTGCTGTGGCTGCAGGTGCCAAGAATGTGGTGAAGCTGCTTGGTGGCCAGAGGATGCAGGACCGAAAGGC ACTGGCCGAGGCCCAGGCCCAGCTCCAGGAATCCTCCCAGAAGCTGGACCTCCTACGGCTGGCCCTGGAA TTGCTGCTGGAGAGGTTGCCTCCCACCCACTCTCTACGTAGCAGGGTGACCCAAGAACTTTGGATGGCAA TGCTTGGGAACCCCCAACCTTTGGGGACACTTGTGAAACCTATTGCCTTGACAGGGACACTGCAGGTTCG CCTCCTGGGCTGCAAAGATCTGCTGGTAGCTGTGCCTGGACGGTCTCCCATGGCTGTCCTGGCAGGTAGC CCTTCTGAAAGCTGGCTCCGAACAAGGTCCCGGCAGCAGCGTGGTGGAGGCGAACTGGCCAGTGAGGTAC TGGCTGTGTTGAAGGTTGACAATCGTGTTGTGGGCCAGACAGGATGGGGACTGGTGGCTGAGAAATCCTG GGACCAGAGTTTTATCATCTCCTTGGACCGAGCTCGCGAGCTGGAGATTGGGGTTCACTGGCGGGACTGG CGACAGTTGTGTGGTGTGGCGTTTCTGAAGCTTGAGGACTTCCTGGACAATGCCTGTCACCAGCTTTCTC TCAGTCTGGTGCCTCAGGGGCGGCTCTTTGCCCAGGTCACCTTCTGCGAGCCTGTCATTGAAAGGAGGCC TCGGCTGCAGAGGCAGAGATGCATTTTCTCTAAGCGGAGAGGCCGGGATTTCATGAGAGCTTCCCAGATG AACCTCAGCATGGCAGCCTGGGGGCGTCTGGTCATGAGCTTGCTGCCCCCCTGCAGCTCACCAAACACAG CCAGTCCCCCTAAAGGGCGCCCTTCAACAGCGGTCTGTGGGACCCCCAGTGCTGCTTCCCCGAGTAACTT CCTGCCCATGAAGACGCTCTCAAAAGAAGACACGAAGCCTCCTCCCAAGCCCCCGCGCCTCTATCTCCAA GAACCAGCCCCAGGGACTCCTTGTACCAAGCGCCCCCACATGGACCCTAGACCTGCAGTAGTGCCCGCCC TGGCAGCCTTATCCACAAGGAAACCCCCCAGACTTCAAGACTTCCGATGTTTGGCTGTGCTGGGTCGGGG ACACTTTGGGAAGGTCCTCCTGGTCCAGTACAAAGGAACAGGGAAATACTACGCTATCAAGGCGTTGAAG AAGCAGGAAGTGCTAGGCCGGGATGAGATTGACAGCCTATACTGTGAGAAGCGGATCCTGGAGACTGTGG GGCGTACAGGGCACCCCTTCCTGCTCTCTCTCCTTGCCTGTCTGCAGACCTCCAGCCATGCCTGCTTTGT TACTGAGTTTCTGCCCGGAGGAGACCTCATGGCGCAGATTCATGAGGATGTCTTTCCTGAGCCCCAGGCC TGCTTCTACCTGGCCTGTGTGGTTCTGGGGCTGCAGTTCCTACACGAGAAGAGGATCATTTACAGGGATC TGAAATTGGATAATCTGCTACTGGATGCCCAGGGTTTCCTCAAGATTGCAGACTTTGGACTATGCAAGGA AGGAATTGGCTTTGGTGACCGGACCAGCACGTTCTGTGGCACCCCGGAGTTCCTGGCCCCCGAGGTTTTG ACTCAGGAGGCTTACACGCGTGCTGTGGACTGGTGGGGGCTGGGTGTGCTGCTCTATGAGATGTTAGTGG GTGAGTGTCCATTCCCGGGGGACACAGAAGAGGAGGTATTTGAATGCATCGTCAGTGCTGATGTCCCATG TCCCCACTTTCTGTCAGTGCAAGGGCTCGAACTCATTCAGAAGCTCCTCCAGAAGTCCCCAGAGAAGCGT CTAGGGGCAGGAGAACGGGACGCCGAGGAGATCAAGGTTCAACCATTCTTCAGGACCACCAACTGGCAGG CCTTGCTGGCCCGCACCGTCCAGCCTCCCTTCGTGCCTACTCTCTGTGGCCCTGCAGACCTTCGTTACTT CGAGGGAGAGTTCACCAGCCTGCCTCCAACCCTGACCCCACCAGTCTCCCAGAGCTCCCTCACTGCTCGC CAACAGGCTGCCTTCCGGGACTTTGACTTCGTGTCTGAGCAGTTCCTGGAGTCCTGAGGGTCTCCTGGCA CTTCAGCCAGACTGCAGGAAGCCTCCACGTAACCATGTGCCTGGGCCGAAGTATTTAATGAGTGTGTGGG AGTCCAGGCAGTGGCCGTCCGTGGGGCTGCCGTTGCAGGCTTTTCCTTCGTGTCCCTGCTGTGTCTATAA CTCTGTCCTTCTATTTCTCGGTAGACCCCAGACCAGGAAAGAGTCCACGGCAGCATGGGTGGCTTTTCTT TTTAATACTTGACTTTATATAGAGCATTAAATTAATAAAACTGTACACTCGAGGTAGCCAGAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4912536 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4912536 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14827 same embryo
	EMAGE:14831 same embryo
	EMAGE:14828 same embryo
	EMAGE:14829 same embryo
	EMAGE:14826 same embryo
	EurExpress:euxassay_005796 same experiment
	MGI:4827236 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source