EMAGE mouse gene expression spatial database

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EMAGE:14831

Thrb thyroid hormone receptor beta ( MGI:98743)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005806. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005806_01	euxassay_005806_02	euxassay_005806_03	euxassay_005806_04

euxassay_005806_05	euxassay_005806_06	euxassay_005806_07	euxassay_005806_08	euxassay_005806_09

euxassay_005806_10	euxassay_005806_11	euxassay_005806_12	euxassay_005806_13	euxassay_005806_14

euxassay_005806_15	euxassay_005806_16	euxassay_005806_17	euxassay_005806_18	euxassay_005806_19

euxassay_005806_20	euxassay_005806_21	euxassay_005806_22	euxassay_005806_23	euxassay_005806_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14831_wholemount_strong.wlz

14831_wholemount_moderate.wlz

14831_wholemount_weak.wlz

14831_wholemount_possible.wlz

14831_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14831_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adenohypophysis

weak

regional

weak expression: see section 10 11 12 13 14 15

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 08 16 17 18 weak expression: see section 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35554
Entity Detected:	Thrb, thyroid hormone receptor beta ( MGI:98743)
Sequence:	sense strand is shown >T35554 GGCAGTGAATCAGCCTTATACCTGTAGTTACCCTGGAAACCTGTTTAAAAGCAAGGACTCTGACTTGGAC ATGGCCCTCAGTCAGTCCAGCCAGCCTGCACATCTCCCTGAAGAAAAGCCTTTTCCTCAAGTGCAGTCGC CACCGCACTCACAAAAAAAAGGGTATATCCCCAGCTATTTAGACAAAGATGAGCTCTGTGTAGTGTGTGG GGACAAAGCCACAGGGTACCACTATCGCTGCATCACCTGTGAAGGCTGCAAGGGCTTCTTTAGAAGAACC ATTCAGAAAAGTCTCCATCCATCTTATTCCTGTAAATATGAAGGAAAGTGCATCATAGACAAAGTCACCC GCAACCAGTGCCAGGAATGTCGCTTTAAGAAATGCATCTATGTTGGCATGGCAACAGACTTGGTGCTGGA TGACAGCAAGAGGCTAGCCAAGCGGAAGCTTATAGAGGAGAACCGTGAGAAGAGGCGGCGGGAAGAGCTA CAGAAATCAATTGGGCACAAGCCAGAACCCACGGATGAGGAGTGGGAGCTCATCAAGACAGTCACTGAGG CCCACGTGGCCACCAATGCCCAGGGCAGCCACTGGAAGCAGAAGCGGAAATTCCTGCCAGAAGACATTGG ACAAGCACCCATTGTGAATGCCCCAGAAGGTGGCAAGGTTGACCTGGAAGCCTTCAGTCATTTTACAAAA ATCATCACACCAGCAATTACCAGAGTGGTGGATTTTGCCAAAAAGTTGCCCATGTTTTGTGAGCTGCCAT GTGAAGACCAGATCATCCTCCTCAAAGGCTGCTGCATGGAGATCATGTCCCTTCGAGCTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 98719. Forward Primer - name:098719_F_cDNA_Thrb, sequence:GGCAGTGAATCAGCCTTATACC; Reverse Primer - name:098719_N_SP6_cDNA_Thrb, sequence:CAGCTCGAAGGGACATGATCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14827 same embryo
	EMAGE:14830 same embryo
	EMAGE:14828 same embryo
	EMAGE:14829 same embryo
	EMAGE:14826 same embryo
	EurExpress:euxassay_005806 same experiment
	MGI:4828704 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source