EMAGE mouse gene expression spatial database

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EMAGE:14860

Taf4a TAF4A RNA polymerase II, TATA box binding protein (TBP)-associated factor ( MGI:2152346)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007946. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007946_01	euxassay_007946_02	euxassay_007946_03	euxassay_007946_04

euxassay_007946_05	euxassay_007946_06	euxassay_007946_07	euxassay_007946_08	euxassay_007946_09

euxassay_007946_10	euxassay_007946_11	euxassay_007946_12	euxassay_007946_13	euxassay_007946_14

euxassay_007946_15	euxassay_007946_16	euxassay_007946_17	euxassay_007946_18	euxassay_007946_19

euxassay_007946_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14860_wholemount_strong.wlz

14860_wholemount_moderate.wlz

14860_wholemount_weak.wlz

14860_wholemount_possible.wlz

14860_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14860_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35542
Entity Detected:	Taf4a, TAF4A RNA polymerase II, TATA box binding protein (TBP)-associated factor ( MGI:2152346)
Sequence:	sense strand is shown >T35542 GATAGGTAAAAAGCACGGCATCACGGAACTGCATCCAGATGTAGTGAGTTACGTGTCTCATGCCACACAG CAAAGGCTGCAGAATCTTGTAGAAAAAATATCTGAAACGGCTCAGCAGAAAAACTTCTCTTATAAGGACG ACGACAGATACGAGCAAGCGAGTGATGTCCGGGCTCAGCTCAAATTCTTTGAACAACTGGATCAGATTGA AAAGCAGAGGAAAGATGAGCAGGAGAGGGAGATCTTAATGCGGGCAGCGAAGTCTCGATCCAGACAAGAA GACCCAGAGCAATTAAGGTTGAAGCAGAAAGCAAAAGAGATGCAGCAGCAGGAGCTGGCACAGATGCGAC AACGAGATGCCAACCTCACTGCACTGGCAGCGATCGGGCCCAGGAAAAAGAGGAAAGTGGACTGCACTGG GCCAGGATCAGGAGCAGAGGGCTCAGGCCCGGGCGCAGCAGTCCCAGGCGGCTCCGGCGTGGGAACCCCC AGACAGTTCACACGGCAAAGAATCACGCGGGTCAACCTCAGGGACCTCATATTTTGTTTAGAAAACGAGC GTGAGACAAGCCATTCACTGTTGCTCTACAAAGCATTCCTTAAGTAACAGGATGAGCCGGCGTTTCTGAT GGGATGCTTGCGGACGTTTTTATATATTTGCAGATTACGCCTTTTTGTAACAAGCAAATGGGATATTGTT TAAAAACAGCCACCTCTTTACAATGGAGCAGTTTTATACTCCTGTTTATAAATCAGCTCTTCAGTGCGGC AGAAACCGTGTCTGCAGCAGAGAGAACACAAAGGCCTGTGGGTGCTCTCACAGGACAGCTGACCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 77017. Forward Primer - name:077017_F_cDNA_Taf4a, sequence:GATAGGTAAAAAGCACGGCATC; Reverse Primer - name:077017_N_SP6_cDNA_Taf4a, sequence:GGGTCAGCTGTCCTGTGAGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14863 same embryo
	EMAGE:14864 same embryo
	EMAGE:14862 same embryo
	EMAGE:14861 same embryo
	EurExpress:euxassay_007946 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source