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EMAGE:15032

Flt4 FMS-like tyrosine kinase 4 ( MGI:95561)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15032 EMAGE:15032 EMAGE:15032 EMAGE:15032 EMAGE:15032
"Pseudo-wholemount" of euxassay_008113. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008113_01 euxassay_008113_02 euxassay_008113_03 euxassay_008113_04
EMAGE:15032 EMAGE:15032 EMAGE:15032 EMAGE:15032 EMAGE:15032
euxassay_008113_05 euxassay_008113_06 euxassay_008113_07 euxassay_008113_08 euxassay_008113_09
EMAGE:15032 EMAGE:15032 EMAGE:15032 EMAGE:15032 EMAGE:15032
euxassay_008113_10 euxassay_008113_11 euxassay_008113_12 euxassay_008113_13 euxassay_008113_14
EMAGE:15032 EMAGE:15032 EMAGE:15032 EMAGE:15032 EMAGE:15032
euxassay_008113_15 euxassay_008113_16 euxassay_008113_17 euxassay_008113_18 euxassay_008113_19
EMAGE:15032 EMAGE:15032 EMAGE:15032 EMAGE:15032 EMAGE:15032
euxassay_008113_20 euxassay_008113_21 euxassay_008113_22 euxassay_008113_23 euxassay_008113_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15032Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15032_wholemount_strong.wlz
15032_wholemount_moderate.wlz
15032_wholemount_weak.wlz
15032_wholemount_possible.wlz
15032_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15032_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36384
Entity Detected:Flt4, FMS-like tyrosine kinase 4 ( MGI:95561)
Sequence:sense strand is shown

>T36384
CAAGACCTGCTTTCTCTGACCTAGTGGAGATCCTGGGGGACCTGCTTCAGGGCGGAGGCTGGCAGGAGGA
GGAAGAGGAGCGCATGGCCCTGCACAGCTCTCAGAGCTCCGAGGAGGATGGCTTCATGCAGGCATCCACC
ACAGCTCTACATATCACCGAAGCAGACGCTGATGATAGTCCACCCAGCATGCATTGCCACAGCCTGGCAG
CCAGATATTACAACTGTGTGTCCTTTCCTGGGCGCCTGGCCAGAGGCACTAAGACTCCAGGCTCTTCCAG
GATGAAGACATTTGAAGAATTGCCCATGACCCCTACAACCTACAAAGCCTCCATGGATAACCAGACAGAC
AGCGGGATGGTGCTGGCCTCAGAAGAGTTTGAGGAGCTAGAAAGCAGGCATAGACCAGAAGGCAGCTTCA
GCTGTAAAGGTCCTGGCCAGCACATGGATATTCCCAGAGGACACCCTGACCCCCAGGGGAGGCGGCGACG
GCCCACTCAAGGGGCACAAGGAGGCAAGGTGTTTTATAACAACGAGTATGGGGAGGTCTCCCAGCCATGT
ACAGAAGGTGACTGCTGCCCGTCTGCTGGCTCCACCTTCTTCGCAGACAGCAGCTACTAAGGAACATGTG
GCAAGGCCTCCTGCCCGTTGCTGCAACCGGCTCTGACAGCTGGGCTGGAGGCCCAGGTTGGGAATTCAAC
TCCTCTGACTCCAGCCCTGGAGGTGTTTTATGTCCCACCCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97729. Forward Primer - name:097729_F_cDNA_Flt4, sequence:CAAGACCTGCTTTCTCTGACCT; Reverse Primer - name:097729_N_SP6_cDNA_Flt4, sequence:GGGGTGGGACATAAAACACCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15028 same embryo
 EMAGE:15027 same embryo
 EMAGE:15031 same embryo
 EMAGE:15030 same embryo
 EMAGE:15029 same embryo
 EurExpress:euxassay_008113 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS