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EMAGE:15187

Fip1l1 FIP1 like 1 (S. cerevisiae) ( MGI:1914149)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15187 EMAGE:15187 EMAGE:15187 EMAGE:15187 EMAGE:15187
"Pseudo-wholemount" of euxassay_010358. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010358_01 euxassay_010358_02 euxassay_010358_03 euxassay_010358_04
EMAGE:15187 EMAGE:15187 EMAGE:15187 EMAGE:15187 EMAGE:15187
euxassay_010358_05 euxassay_010358_06 euxassay_010358_07 euxassay_010358_08 euxassay_010358_09
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euxassay_010358_10 euxassay_010358_11 euxassay_010358_12 euxassay_010358_13 euxassay_010358_14
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euxassay_010358_15 euxassay_010358_16 euxassay_010358_17 euxassay_010358_18 euxassay_010358_19
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euxassay_010358_20 euxassay_010358_21 euxassay_010358_22 euxassay_010358_23 euxassay_010358_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15187Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15187_wholemount_strong.wlz
15187_wholemount_moderate.wlz
15187_wholemount_weak.wlz
15187_wholemount_possible.wlz
15187_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15187_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30196
Entity Detected:Fip1l1, FIP1 like 1 (S. cerevisiae) ( MGI:1914149)
Sequence:sense strand is shown

>T30196
CAAGGCAGGGGGAAGAGTTTATGGAAATACAGGAACCAAAGTCAAAGGAGTGGACCTCGATGCACCTGGC
AGCATTAATGGAGTTCCACTCTTGGAAGTAGATCTGGATTCTTTTGAAGATAAACCATGGCGGAAACCTG
GTGCTGATCTTTCTGATTATTTTAACTATGGCTTTAATGAAGATACTTGGAAAGCTTACTGTGAAAAACA
AAAGAGGATACGAATGGGACTGGAAGTTATACCTGTTACTTCAACCACAAACAAGATTACGGCCGAAGAC
TGTACTATGGAAGTTACACCAGGTGCAGAGATCCAAGATGGCAGATTCAATCTTTTTAAGGTACAGCAAG
GGAGAACTGGAAATTCAGAGAAAGAAGCAGCACTTCCATCTACAAAAGCTGAGTTTACTTCTCCGCCATC
ATTGTTCAAGACTGGTCTGCCACCAAGCAGAAACAGCACCTCTTCTCAGTCTCAGACAAGTACTGCCTCC
AGAAAAGCCAGTTCAAGCGTTGGGAAGTGGCAGGATCGATATGGGAGGGCCGAGTCACCTGATCTAAGGA
GATTACCTGGAGCAATTGATGTCATTGGTCAAACAATAACCATCAGCCGAGTGGAAGGAAGACGGCGTGC
AAATGAGAACAGCAATATACAGGTCCTTTCTGACAGATCTGCCACTGAAGTAGACAACAATTTCAGCAAA
CCACCTCCATTTTTCCCTCCAGGGGCTCCTCCTACTCACCTTCCTCCTCCTCCATTTCTCCCACCACCTC
CAACTGTCAGCACCGCCCCACCTCTCATTCCACCACCAGGTTTCCCCCCTCCACCAGGCGCCCCACCTCC
ATCTCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3499130), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 14322. Forward Primer - name:014322_F_IRAV04-07_N23_Fip1l1, sequence:CAAGGCAGGGGGAAGAGT; Reverse Primer - name:014322_R_SP6_IRAV04-07_N23_Fip1l1, sequence:AAGAGATGGAGGTGGGGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15189 same embryo
 EMAGE:15186 same embryo
 EMAGE:15185 same embryo
 EMAGE:15184 same embryo
 EMAGE:15188 same embryo
 EurExpress:euxassay_010358 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS