EMAGE mouse gene expression spatial database

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EMAGE:15188

Tnfrsf12a tumor necrosis factor receptor superfamily, member 12a ( MGI:1351484)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010367. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010367_01	euxassay_010367_02	euxassay_010367_03	euxassay_010367_04

euxassay_010367_05	euxassay_010367_06	euxassay_010367_07	euxassay_010367_08	euxassay_010367_09

euxassay_010367_10	euxassay_010367_11	euxassay_010367_12	euxassay_010367_13	euxassay_010367_14

euxassay_010367_15	euxassay_010367_16	euxassay_010367_17	euxassay_010367_18	euxassay_010367_19

euxassay_010367_20	euxassay_010367_21	euxassay_010367_22	euxassay_010367_23	euxassay_010367_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15188_wholemount_strong.wlz

15188_wholemount_moderate.wlz

15188_wholemount_weak.wlz

15188_wholemount_possible.wlz

15188_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15188_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

humerus

strong

regional

strong expression: see section 02 03 04 19 20 21

femur

strong

regional

strong expression: see section 11 19 20

adrenal medulla

moderate

regional

moderate expression: see section 08 09 16 17

aorta

moderate

regional

moderate expression: see section 09 10 11 12 13

heart ventricle

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16

tongue muscle

moderate

regional

moderate expression: see section 11 13 14

lower jaw molar

moderate

regional

moderate expression: see section 07 18

upper jaw molar

moderate

regional

moderate expression: see section 07 18

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30249
Entity Detected:	Tnfrsf12a, tumor necrosis factor receptor superfamily, member 12a ( MGI:1351484)
Sequence:	sense strand is shown >T30249 GTGTTGGGATTCGGCTTGGTGTTGATGCGCGCCGCGGCCGGGGAGCAAGCACCAGGCACCTCCCCATGCT CTAGCGGCAGCTCCTGGAGCGCGGACCTCGACAAGTGCATGGACTGCGCTTCTTGTCCAGCGCGACCACA CAGCGACTTCTGCCTGGGATGCGCCGCAGCACCTCCTGCCCACTTCAGGCTACTGTGGCCCATTCTGGGG GGCGCTCTTAGTCTGGTCCTGGTTTTGGCGCTGGTTTCTAGTTTCCTGGTCTGGAGAAGATGCCGCCGGA GAGAAAAGTTTACTACCCCCATAGAGGAGACTGGTGGAGAGGGCTGCCCAGGTGTGGCACTGATCCAGTG AGGAGCACCCGCGCTGGGGCCCACTCGTCGTCCATTCATTCATTCTGGAGTCAGCCTGGCCTTCCAGACA GAAGCCGAGCCAGACTCTTTCAACCACAAGGGGGTGGGGCGAGGTGGTGATTCACCTCCAAGGACTGGGC TTAGAGTTCAGGGAGCCTTCCAAGGTGTCCAATTGCCCTATCTCTGGGTCTGGGGCAGACAGAGAGCCTC AATCTGGGTCACAAAGCGACCCATACTAAGGAACTGCAGCATTTGCACAAGGGAATCTCTTGTTCCCCAC AAGTCCTGGCAGTCTGGCTGACTTGAGGGGCAGACACAACACTGGGTCCCACCCACTCGGAGGGGCTATA ATGCCACTCCCTGAGTTTTAGCCCTAGGAGTGGGTCAGATTGACAGACCTGTTCTTAACACTAAGGGGGC TGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5251646), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 26171. Forward Primer - name:026171_F_IRAV63-66_I04_Tnfrsf12a, sequence:GTGTTGGGATTCGGCTTG; Reverse Primer - name:026171_R_SP6_IRAV63-66_I04_Tnfrsf12a, sequence:GCCAGCCCCCTTAGTGTT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15189 same embryo
	EMAGE:15187 same embryo
	EMAGE:15186 same embryo
	EMAGE:15185 same embryo
	EMAGE:15184 same embryo
	EurExpress:euxassay_010367 same experiment
	MGI:4828842 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source