Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:15205

Hap1 huntingtin-associated protein 1 ( MGI:1261831)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15205 EMAGE:15205 EMAGE:15205 EMAGE:15205 EMAGE:15205
"Pseudo-wholemount" of euxassay_010402. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010402_01 euxassay_010402_02 euxassay_010402_03 euxassay_010402_04
EMAGE:15205 EMAGE:15205 EMAGE:15205 EMAGE:15205 EMAGE:15205
euxassay_010402_05 euxassay_010402_06 euxassay_010402_07 euxassay_010402_08 euxassay_010402_09
EMAGE:15205 EMAGE:15205 EMAGE:15205 EMAGE:15205 EMAGE:15205
euxassay_010402_10 euxassay_010402_11 euxassay_010402_12 euxassay_010402_13 euxassay_010402_14
EMAGE:15205 EMAGE:15205 EMAGE:15205 EMAGE:15205 EMAGE:15205
euxassay_010402_15 euxassay_010402_16 euxassay_010402_17 euxassay_010402_18 euxassay_010402_19
EMAGE:15205 EMAGE:15205 EMAGE:15205 EMAGE:15205 EMAGE:15205
euxassay_010402_20 euxassay_010402_21 euxassay_010402_22 euxassay_010402_23 euxassay_010402_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15205Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15205_wholemount_strong.wlz
15205_wholemount_moderate.wlz
15205_wholemount_weak.wlz
15205_wholemount_possible.wlz
15205_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15205_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
adrenal gland
moderate moderate
regionalmoderate expression: see section 08 09 10 16 17
thyroid gland
weak weak
regionalweak expression: see section 10 14
forebrain
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
pituitary gland
weak weak
regionalweak expression: see section 10 11 12 13 14 15
hindbrain
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
midbrain
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19
vagus x ganglion
weak weak
regionalweak expression: see section 06 07 17
trigeminal v nerve
moderate moderate
regionalmoderate expression: see section 09 16
spinal cord
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 09 14 15
cervical ganglion
moderate moderate
regionalmoderate expression: see section 08 16
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 11 12 13 14
naris
weak weak
regionalweak expression: see section 13
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 08 09 10 11 12 14 15 16 17 18 19
vomeronasal organ
weak weak
regionalweak expression: see section 12
stomach
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09 10
midgut
weak weak
regionalweak expression: see section 09 10 11 12 13 14 15
lower jaw incisor
weak weak
regionalweak expression: see section 10 11 14 15 16
lower jaw molar
weak weak
regionalweak expression: see section 19
upper jaw incisor
weak weak
regionalweak expression: see section 11 15 16
upper jaw molar
weak weak
regionalweak expression: see section 06 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30236
Entity Detected:Hap1, huntingtin-associated protein 1 ( MGI:1261831)
Sequence:sense strand is shown

>T30236
CGTATTTCATGGAGAGACGTGACACTCACTGCAGGGAGGGGCGGAAGAAGGAGCAGAGGGCGATGCCACC
CCCACCGGCTCAAGATCTCAAGCCACCTGAAGATTTTGAGGCTCCAGAGGAGTTGGTTCCTGAGGAGGAG
CTGGGGGCCATCGAAGAGGTGGGGACAGCTGAGGATGGGCAGGCAGAAGAGAACGAGCAGGCGTCTGAGG
AGACCGAGGCCTGGGAGGAGGTGGAACCGGAGGTGGACGAGACCACAAGGATGAATGTGGTGGTCTCCGC
CCTGGAGGCCAGTGGCCTGGGCCCTTCCCACCTGGACATGAAGTATGTCCTCCAGCAACTGTCCAACTGG
CAGGACGCCCACTCTAAGCGGCAGCAGAAGCAGAAGGTGGTCCCAAAAGACTCCCCAACCCCGCAGCAGC
AAACAAACATGGGGGGCGGGATCCTGGAGCAGCAGCCCAGAGTGCCGACCCAGGACTCTCAGAGGCTGGA
GGAGGACAGGGCCACTCACTCTCCCAGTGCCAGGGAGGAAGAGGGGCCTTCTGGGGCCACCTAGGCCTGG
AACGAAGGCCTCTGCCAGCAAGGGCCACAGTTGGACCATTCCCCCGGCTCCTTTCGTCTTCATGGCCCTG
CGGGTGGGTACATGTGATTGTGCACCATTTATTAGAAGAGGAACCCGAAGTTCAGAGATCTGAGATGACT
TGGCCAAGGTCATACTAGGAGCAGATCTGAGCCCGTGTCTATGAGTTCTGAACCCCTGGCCTGGTCTCAC
TGCTGTCCGCACCTCCTGCTCCCATCTTTGCGTTCCACCTGAAGTTTTGGCAGCCTCCTTCAGTCGTTCT
GATGCCCCCACCCCCGTTTTGGGGTGCACCAATGGGTGGAACATCCCTGGCTGCGGCACAGTAAGATGGC
GCAGGTTGTAAGCTGGGTCAGCACTTCCGTGGCTGACCACGGGGGACCTGAGTGAGGGCCAGTGGTGGTT
TTCTGTCGTCTCCTCTGTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4481735), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 8149. Forward Primer - name:ABA_MGC_035_02_B06_F_TTTTTGCCCACTTGCTCC_ABA_FR_035, sequence:CGTATTTCATGGAGAGACGTGA; Reverse Primer - name:_R_SP6_ABA_MGC_011_02_A07_CGTATTTCATGGAGAGACGTGACA, sequence:TGACAGAGGAGACGACAGAAAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15207 same embryo
 EMAGE:15210 same embryo
 EMAGE:15208 same embryo
 EMAGE:15206 same embryo
 EMAGE:15209 same embryo
 EurExpress:euxassay_010402 same experiment
 MGI:4825320 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS