EMAGE mouse gene expression spatial database

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EMAGE:15209

Six1 sine oculis-related homeobox 1 homolog (Drosophila) ( MGI:102780)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010410. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010410_01	euxassay_010410_02	euxassay_010410_03	euxassay_010410_04

euxassay_010410_05	euxassay_010410_06	euxassay_010410_07	euxassay_010410_08	euxassay_010410_09

euxassay_010410_10	euxassay_010410_11	euxassay_010410_12	euxassay_010410_13	euxassay_010410_14

euxassay_010410_15	euxassay_010410_16	euxassay_010410_17	euxassay_010410_18	euxassay_010410_19

euxassay_010410_20	euxassay_010410_21	euxassay_010410_22	euxassay_010410_23	euxassay_010410_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

EMAGE:15209

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15209_wholemount_strong.wlz

15209_wholemount_moderate.wlz

15209_wholemount_weak.wlz

15209_wholemount_possible.wlz

15209_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15209_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 13 14 15 16 17 18 19 20

foot

moderate

regional

moderate expression: see section 05 06

hindlimb digit 1

moderate

regional

moderate expression: see section 20 21 22

hindlimb digit 2

moderate

regional

moderate expression: see section 20 21 22

hindlimb digit 3

moderate

regional

moderate expression: see section 20 21 22

hindlimb digit 4

moderate

regional

moderate expression: see section 20 21 22

hindlimb digit 5

moderate

regional

moderate expression: see section 20 21 22

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 14

pituitary gland

strong

regional

strong expression: see section 10 11 12 13 14 15

tongue

weak

regional

weak expression: see section 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30237
Entity Detected:	Six1, sine oculis-related homeobox 1 homolog (Drosophila) ( MGI:102780)
Sequence:	sense strand is shown >T30237 CTCGGTCCTTCTGCTCCAGAGCAATATGGGCCACGCCAGGAGCTCAAACTATTCTCTTCCAGGCCTCACA GCCTCCCAGCCCAGCCACGGTCTGCAAGCCCATCAGCACCAGCTCCAGGACTCTCTGCTGGGCCCACTCA CCTCCAGTTTGGTGGACTTGGGTTCCTAAGTGGGGAGATATTGGGGCCTTGAAGGGATGGCCGGAGCAGC AACCAACCGCAGCAATCTAGGGGACACTTGTACATAGAAGCCAGGGACAATTCTGCAGCTTGTTTCTGGG GTTCTTTGCGCAGAAAGGAACCACAGGCTTTCACAATTGTCTTTTTAAAAATTACAACCAACAGCGGTCT CAGTTGAGCATCCTCTCCCTCCTCTCCAACTCTTAATACCCTTGCATTTCCCCTCCCACTAAAAGGTCAG GAAATAACAAAAACAGCCAGGCTTGGTTCTGGGCAACCCAGGCATCCTTTACAGTAGTCCCTTATTCCAC CTCTTCTTCCTCCTCCTCCTCGTCTTCTTTTAATGAACAGAAATTACAAATCAGCTGGATTGTAATTATT TTTTTAATTTATTTATGGAGAAACGGTTTAGGGAAGAGGAAAAGGAAATGAAGAGAGATCAAAATAATGA AAACAAGAAACCTTCAGTGTGAGGGGAACTGGCTGCCTTCTTAAGACAAATAGGAAAACTATAATCACAG CTGTTTTTTGATGGGAAGATTAAGTTTACATTTTTCATATTTAGTGTTAAATAATTTTATGTAGATTAAA ATAAAAGAGCAGTATTAGCAAGGGAAACAAGTGCCTAAATGTCTTAATGCTCTCTATGGGAGGCGGTTAG GGTAGCAGCG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4188451), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 25581. Forward Primer - name:025581_F_IRAV59-62_O15_Six1, sequence:CTCGGTCCTTCTGCTCCA; Reverse Primer - name:025581_R_SP6_IRAV59-62_O15_Six1, sequence:TCGCTGCTACCCTAACCG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15207 same embryo
	EMAGE:15205 same embryo
	EMAGE:15210 same embryo
	EMAGE:15208 same embryo
	EMAGE:15206 same embryo
	EurExpress:euxassay_010410 same experiment
	MGI:4828079 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source