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EMAGE:15243

Grap2 GRB2-related adaptor protein 2 ( MGI:1333842)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15243 EMAGE:15243 EMAGE:15243 EMAGE:15243 EMAGE:15243
"Pseudo-wholemount" of euxassay_010388. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010388_01 euxassay_010388_02 euxassay_010388_03 euxassay_010388_04
EMAGE:15243 EMAGE:15243 EMAGE:15243 EMAGE:15243 EMAGE:15243
euxassay_010388_05 euxassay_010388_06 euxassay_010388_07 euxassay_010388_08 euxassay_010388_09
EMAGE:15243 EMAGE:15243 EMAGE:15243 EMAGE:15243 EMAGE:15243
euxassay_010388_10 euxassay_010388_11 euxassay_010388_12 euxassay_010388_13 euxassay_010388_14
EMAGE:15243 EMAGE:15243 EMAGE:15243 EMAGE:15243 EMAGE:15243
euxassay_010388_15 euxassay_010388_16 euxassay_010388_17 euxassay_010388_18 euxassay_010388_19
EMAGE:15243 EMAGE:15243 EMAGE:15243
euxassay_010388_20 euxassay_010388_21 euxassay_010388_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15243Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15243_wholemount_strong.wlz
15243_wholemount_moderate.wlz
15243_wholemount_weak.wlz
15243_wholemount_possible.wlz
15243_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15243_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
liver
moderate moderate
spottedmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30515
Entity Detected:Grap2, GRB2-related adaptor protein 2 ( MGI:1333842)
Sequence:sense strand is shown

>T30515
GCCTCAGGTGAGGACGAACTGAGCTTTCGCACTGGAGACATTCTGAAGATACTAAGCAACCAGGAGGAGT
GGCTCAAGGCGGAGCTCGGAAGCCAAGAAGGATATGTGCCTAAGAATTTTATAGATATCGAGTTTCCTGA
GTGGTTCCATGAAGGCCTCTCTCGACATCAAGCAGAAAACTTACTCATGGGCAAGGACATTGGATTCTTC
ATCATCAGGGCCAGCCAGAGTTCCCCAGGAGACTTCTCCATCTCTGTCAGGCACGAGGACGACGTTCAGC
ACTTCAAAGTCATGCGTGACACCAAGGGCAATTACTTCCTGTGGACCGAGAAGTTCCCATCCTTAAATAA
GCTGGTGGACTACTACCGGACAACGTCCATCTCCAAACAGAAGCAGGTCTTCCTTCGGGATGGAACCCAA
GATCAGGGTCACCGGGGCAACAGCCTGGACAGGAGGTCCCAGGGCGGCCCTCACCCGAGTGGAACCGTAG
GAGAAGAAATCCGACCTTCAGTGAACCGGAAGCTGTCAGACCACCTTCCTCTGGGTCCCCAGCAGTTCCA
CCCGCACCAGCAGCCGTCACCCCAGTTCACACCAGGGCCGCAGCCCCCTCAACAGCAGCGTTATCTGCAG
CATTTCCACCAGGACCGTCGTGGGGGCAGCCTGGACATAAACGATGGACACTGCGGCCTTGGAAGTGAGG
TGAACGCCACGCTGATGCACCGGAGACACACCGACCCCGTACAACTCCAGGCAGCAGGGCGGGTGCGTTG
GGCCAGGGCACTGTATGACTTTGAGGCTCTGGAAGAGGACGAGCTGGGATTCCGAAGCGGAGAAGTGGTT
GAAGTCCTGGACAGCTCCAACCCATCTTGGTGGACCGGCCGTCTGCACAACAAACTGGGTCTCTTCCCTG
CCAACTATGTGGCCCCCATGATGCGATGAGTCGTACTGCAAGGCTTAGGGGCTTTTCGTCCAAAGCCGCC
TGCAAGACAGAGAGGGGCG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:1446918), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59305. Forward Primer - name:059305_F_IRAV110_b02_Grap2, sequence:GCCTCAGGTGAGGACGAA; Reverse Primer - name:059305_R_SP6_IRAV110_b02_Grap2, sequence:TCGCCCCTCTCTGTCTTG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15241 same embryo
 EMAGE:15242 same embryo
 EMAGE:15244 same embryo
 EMAGE:15245 same embryo
 EMAGE:15246 same embryo
 EurExpress:euxassay_010388 same experiment
 MGI:4825214 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS